Associations between antinuclear antibody staining patterns and clinical features of systemic lupus erythematosus: analysis of a regional Swedish register

Frodlund, Martina; Dahlström, Örjan; Kastbom, Alf; Skogh, Thomas; Sjöwall, Christopher

doi:10.1136/bmjopen-2013-003608

Cited by 60 publications

(77 citation statements)

References 54 publications

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“…Frodlund ve ark. 'ları immünfloresan yöntemiyle ANA pozitifliğinin devam ettiği SLE tanılı hastalar arasında yaptıkları çalışmada ANA paternlerini %62 oranında homojen ± diğer patern, %10'unda sadece granüler patern olarak rapor etmişlerdir (21). Bizim çalışmamızda immünfloresan yöntemiyle ANA paterni değerlendirilen SLE tanılı hastaların %35,8'i homojen patern, %22,2'si homojen patern + diğer paternler, %24,7'si granüler patern, %8,7'si granüler + diğer paternler, %7,4'ü sadece nükleolar patern ve %1,2'si sadece sentromer patern olarak bulunmuştur.…”

Section: Discussionunclassified

Comparison of three ELISA methods with CLIF test for detection of Anti-dsDNA antibody

Cetin¹,

Toyran²,

Aytaç³

et al. 2015

Turk Hij Den Biyol Derg

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Objective: Systemic lupus erythematosus (SLE) is an autoimmune disease caused by antibodies which produced against antigens commonly on cell nuclei. According to the criteria of American College of Rheumatology (ACR), the immunological parameters which are used for diagnosis of SLE, are anti-nuclear antibodies (ANA) and anti-dsDNA autoantibodies. In this study it is aimed to investigate the specifity of three different ELISA tests by comparing with CLIF test, as a reference method, for the determination of anti-dsDNA antibodies.Methods: Sera of 81 patients who were diagnosed as SLE and sent to the Microbiology Department of our hospital between 1 May -31 July 2013, were included in the study. In these sera, ANA positivity was firstly investigated by immunofluorescence antibody test (IFA). Positive sera were stored at -80 O C for prospective analysis and processed with four different anti-dsDNA assays at the same day. These assays were three antidsDNA ELISA kits including; CHORUS dsDNA-G (DIESSE Diagnostica Senese, Italy), anti-dsDNA-Ncx ELISA IgG (EUROIMMUN, Germany, California), QUANTA Lite dsDNA SC ELISA (INOVA Diagnostics) and CLIF test (Crithidia luciliae anti-dsDNA, EUROIMMUN, Germany). Makale Dili "Türkçe"/Article Language "Turkish" Results

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Section: Discussionunclassified

Comparison of three ELISA methods with CLIF test for detection of Anti-dsDNA antibody

Cetin¹,

Toyran²,

Aytaç³

et al. 2015

Turk Hij Den Biyol Derg

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“…Keadaan ini berbeda dengan Frodlund dkk. 8 yang mengemukakan tidak ada perbedaan keterlibatan gangguan hematologi pada berbagai pola ANA. Pola homogen diduga berkaitan dengan anti-dsDNA, anti-histon, dan juga antikompleks DNA/histon.…”

Section: Pembahasanunclassified

Departemen Ilmu Kesehatan Masyarakat Fakultas Kedokteran Universitas Padjadjaran

Ghrahani¹,

Setiabudiawan²,

Sapartini³

2015

mkb

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AbstrakLupus eritematosus sistemik (LES) adalah penyakit autoimun kronik yang melibatkan berbagai sistem organ ditandai dengan produksi berbagai autoantibodi. Penyakit ini memiliki manifestasi klinis yang sangat bervariasi. Antibodi antinuklear diketahui memiliki pola-pola tertentu yang diduga berkorelasi dengan keterlibatan sistem organ tertentu pada LES. Penelitian ini dilakukan untuk melihat hubungan pola antibodi antinuklear (ANA) dengan keterlibatan berbagai sistem organ pada anak yang menderita LES. Studi potong lintang dilakukan terhadap 93 anak dengan diagnosis LES yang datang ke Departemen Ilmu Kesehatan Anak Fakultas Kedokteran Universitas Padjajaran/Rumah Sakit Dr. Hasan Sadikin Bandung, pada periode September 2006-April 2015. Analisis data dilakukan dengan uji chi-kuadrat dan uji-t. Subjek terdiri atas 85 (91%) perempuan dan 8 (9%) lakilaki, dengan rasio perempuan:laki-laki adalah 10,6:1. Usia rata-rata adalah 10,5±3 tahun dan rentang usia 2-17 tahun. Pola ANA terbanyak adalah speckled (58%) dan homogen (19%). Subjek dengan pola ANA homogen lebih berisiko mengalami keterlibatan hematologi yaitu anemia (OR 4,8; IK 95%: 1,1-19) dan leukopenia (OR 3,9; IK 95%: 2,0-7,5) dibanding subjek dengan pola ANA bukan homogen. Tidak didapatkan hubungan pola ANA dengan keterlibatan sistem organ lain. Titer antidsDNA pada subjek dengan pola ANA homogen lebih tinggi dibanding subjek dengan pola ANA bukan homogen (p=0,02). Simpulan, subjek dengan pola ANA homogen memiliki risiko lebih besar mengalami keterlibatan hematologi dibanding dengan pola ANA yang lain. Antinuclear Antibody Pattern as a Risk Factor in Hematological System Involvement in Pediatric Systemic Lupus Erythematosus AbstractSystemic lupus erythematosus (SLE) is a chronic autoimmune disease that can involve any organ system with the evidence of autoantibody production. The disease has a wide range of clinical manifestation. Antinuclear antibody is known to have particular staining patterns and suspected have a correlation with multiorgan involvement. The objective of this study was to define antinuclear antibody (ANA) staining pattern correlation from multiorgan involvement in 93 children with SLE. This was a cross-sectional study conducted at the Department of Child Health, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital Bandung during the period of September 2006 to April 2015. Data were analyzed using chi-square test and t-test. This study involved 93 children with SLE, consisted of 85 (91%) females and 8 (9%) males, with a ratio of 10.6:1. Mean age was 10.5±3 years with age range of 2 to 17 years. The most frequent ANA staining patterns were speckled (58%) and homogenous (19%). Subjects with homogenous pattern have a higher hematology involvement risk, which are anemia (OR: 4.8, CI 95%, 1.1-19) and leukocytopenia (OR 3.9, 95% CI 2.0-7.5). Subjects with homogenous ANA pattern had a higher titer of anti-dsDNA than those with other patterns (p=0.02). In conclusion, subjects with homogenous pattern have a higher hematolo...

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“…The ACR-82 criteria are shown in Table I and exemplify typical manifestations of SLE. Among the most common manifestations of SLE (>30% of patients) are arthritis/arthralgia, malar rash, fever and photosensitivity whereas epilepsy, psychosis, and oral ulcers constitute relatively rare manifestations [4,8]. In order to fulfil the ACR-82 criteria, at least four of the listed conditions must be met (Table I) [6].…”

Section: Clinical Characteristics Classification Criteria and Diagnosismentioning

confidence: 99%

“…A prolonged extracellular exposure of nuclear components can cause structural alterations which may result in immunogenic epitopes that are able to activate an immune response [13]. ANAs are examples of autoantibodies that are characteristic of SLE and include the anti-dsDNA, antibodies to U1 small nuclear ribonucleoproteins (snRNPs), anti-Smith (anti-Sm), anti-Ro/SSA and antiLa/SSB autoantibodies [8]. Interestingly, there are also studies showing an overrepresentation in SLE of autoantibodies against proteins that are involved in the elimination of cell debris, like C1q [23], serum amyloid P (SAP) [24], and CRP [25,26] (further described in the CRP section).…”

Section: Autoantibodiesmentioning

confidence: 99%

Biomarkers and mediators in systemic lupus erythematosus : IFNα versus the CRP response, and evaluation of suPAR and anti-dsDNA antibody assays

Enocsson¹

2014

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Systemic lupus erythematosus (SLE) is a heterogeneous autoimmune disease which may affect multiple organ systems. Interferon alpha (IFNα) and autoantibodies that form immune complexes with nuclear antigens (ANA) are hallmarks believed to drive the disease into a vicious circle of inflammation, tissue damage, autoantigen exposure and autoantibody production.In SLE, the disease course is characterized by episodes of exacerbations alternating with remissions. In order to best treat the patient it is important to closely monitor symptoms and signs of disease activity. Because of the disease heterogeneity, no single biomarker has yet been found to reflect SLE disease activity in general, although anti-double stranded DNA (anti-dsDNA) antibodies sometimes indicate activity, primarily with renal involvement, and constitutes an item of the SLE disease activity score SLEDAI-2K. However, the method of anti-dsDNA measurement is not standardized and therefore varies between different laboratories. In many other inflammatory conditions, such as rheumatoid arthritis and during bacterial infections, the C-reactive protein (CRP) level is a good indicator of ongoing inflammation, but in SLE and during viral infections, CRP commonly fails to reflect the degree of inflammation. Both viral infections and SLE are characterized by IFNα, and we thus aimed to elucidate whether IFNα can inhibit CRP production. Further, four assays for anti-dsDNA antibody measurements were evaluated with regard to SLE disease specificity and activity, and a new potential biomarker of inflammation, the soluble urokinase plasminogen activator receptor (suPAR), was assessed in relation to disease activity and organ damage.An in vitro inhibitory effect of IFNα on CRP transcription and production was found in hepatocytes, and this was consolidated by in vivo studies of CRP and IFNα in sera from well-characterized SLE patients (KLURING; Kliniskt lupusregister i nordöstra Götaland). Here, CRP and disease activity were associated among patients without IFNα and without a CRP lowering gene variant (SNP rs1205). The poor disease activity compliance of CRP could therefore be explained, at least in part, by polymorphisms in the CRP gene and increased levels of IFNα. Critical differences between the methods measuring anti-dsDNA were found regarding disease specificity and ability to reflect disease activity and the results suggests the Crithidia luciliae immunofluorescence test (CLIFT) for diagnostic purposes and a bead-based multiplex assay (FIDIS) for monitoring of disease activity.

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Associations between antinuclear antibody staining patterns and clinical features of systemic lupus erythematosus: analysis of a regional Swedish register

Cited by 60 publications

References 54 publications

Comparison of three ELISA methods with CLIF test for detection of Anti-dsDNA antibody

Comparison of three ELISA methods with CLIF test for detection of Anti-dsDNA antibody

Departemen Ilmu Kesehatan Masyarakat Fakultas Kedokteran Universitas Padjadjaran

Biomarkers and mediators in systemic lupus erythematosus : IFNα versus the CRP response, and evaluation of suPAR and anti-dsDNA antibody assays

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