Associations between Listeria monocytogenes genomic characteristics and adhesion to polystyrene at 8 °C

Mahoney, David Burke James; Falardeau, Justin; Hingston, Patricia; Chmielowska, Cora; Carroll, Laura M.; Jang, Sung Sik; Wang, Siyun

doi:10.1016/j.fm.2021.103915

Cited by 14 publications

(7 citation statements)

References 36 publications

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“…Although not presenting a Premature Stop Codon Mutation in the inlA gene nor the SSI-1, both associated with an increased production of biofilm, as reported in previous studies (Keeney et al, 2018;Upham et al, 2019;Mahoney et al, 2022), all the strains carried several genetic markers for biofilm production. In particular luxS, for a S-ribosylhomocysteinase, recO encoding the DNA repair protein RecO and lmo2504 for a cell wall-binding protein were found in all the genomes (Pasquali et al, 2018;Gorski et al, 2022).…”

Section: Discussionsupporting

confidence: 73%

Atypical Serogroup IVb-v1 of Listeria monocytogenes Assigned to New ST2801, Widely Spread and Persistent in the Environment of a Pork-Meat Producing Plant of Central Italy

et al. 2022

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In this study, we characterized 84 Listeria monocytogenes (Lm) strains having an atypical IVb-v1 profile and isolated in a meat producing plant of Central Italy. They were assigned to the new MLST type ST2801 (CC218). The new ST was widespread in the food-producing environment where it was able to persist for over a year even after cleaning and sanitation. Cluster analysis identified three main clusters genetically close to each other (0–22 allelic differences and 0–28 SNPs) from two different cgMLST types, suggesting a common source. The coexistence of closely related clusters over time could be the result of a different evolution path starting from a common ancestor first introduced in the plant and/or the consequence of the repetitive reintroduction of closely related clones probably by raw materials. All the strains presented several determinants for heavy metals resistance, stress response, biofilm production, and multidrug efflux pumps with no significant differences among the clusters. A total of 53 strains carried pLI100 and the j1776 plasmids, while in one strain, the pLM33 was found in addition to pLI100. Only the strains carrying plasmids presented cadA and cadC for cadmium resistance and the mco gene encoding a multicopper oxidase and gerN for an additional Na+/H+-K+ antiporter. All the strains presented a virulence profile including a full-length inlA gene and the additional LIPI-3. The isolation of a new ST with a large pattern of stress-adaptation genes and able to persist is an important contribution to deepening the current knowledge on the uncommon IVb-v1 and in general on the genomic diversity of Lm.

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Section: Discussionsupporting

confidence: 73%

Atypical Serogroup IVb-v1 of Listeria monocytogenes Assigned to New ST2801, Widely Spread and Persistent in the Environment of a Pork-Meat Producing Plant of Central Italy

et al. 2022

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“…Another genetic determinant associated with CCs commonly found in food processing plants is premature stop codon (PMSC) truncation mutations in inlA encoding the virulence factor internalin A ( 41 , 42 ). Although the ecological significance of inlA PMSC mutations is not fully understood, some studies indicate that they mediate increased adhesion and biofilm formation ( 43 – 45 ) and increased tolerance to desiccation ( 27 ).…”

Section: Introductionmentioning

confidence: 99%

Pervasive Listeria monocytogenes Is Common in the Norwegian Food System and Is Associated with Increased Prevalence of Stress Survival and Resistance Determinants

Fagerlund

Wagner

Møretrø

et al. 2022

Appl Environ Microbiol

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“…Previous studies have also observed a high prevalence of inlA PMSCs and SSI-1 among LM showing persistence characteristics in food processing plants (Alvarez-Molina et al, 2021; Kaszoni-Rückerl et al, 2020; Palaiodimou et al, 2021), which may suggest that these genetic markers confer certain phenotypic characteristics that contribute to LM persistence in built environments. For example, presence of inlA PMSCs has been reported to be associated with enhanced adherence of LM to polystyrene (Mahoney et al, 2022) and stainless steel surfaces (Piercey et al, 2016) under low temperature storage conditions, and SSI-1 has been reported to enhance LM tolerance to stress conditions such as low pH and high salt concentrations (Ryan et al, 2010). However, these studies were carried out in vitro , so there are still knowledge gaps associated with the specific role that inlA PMSCs and SSI-1 play in potentially contributing to LM persistence in food processing environments.…”

Section: Discussionmentioning

confidence: 99%

Intensive environmental sampling and whole genome sequence-based characterization ofListeriain small and medium sized dairy plants reveal opportunities for simplified and size-appropriate environmental monitoring strategies

Bolten,

Lott,

Ralyea

et al. 2024

Preprint

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Small and medium sized dairy processing plants (SMDPs) may face unique challenges with respect to controlling Listeria in their processing environments, e.g., due to limited resources. The aim of this study was to implement and evaluate environmental monitoring programs (EMPs) for Listeria control in eight SMDPs in a ~1-year longitudinal study; this included a comparison of pre-operation (i.e., after cleaning and sanitation and prior to production) and mid-operation (i.e., at least 4 h into production) sampling strategies. Among 2,072 environmental sponge samples collected across all plants, 272 (13%) were positive for Listeria. Listeria prevalence among pre- and mid-operation samples (15 and 17%, respectively), was not significantly different. Whole genome sequencing (WGS) performed on select isolates to characterize Listeria persistence patterns revealed repeated isolation of closely related Listeria isolates (i.e., ≤20 high quality single nucleotide polymorphism [hqSNP] differences) in 5/8 plants over >6 months, suggesting Listeria persistence and/or re-introduction was relatively common among the SMDPs evaluated here. WGS furthermore showed that for 41 sites where samples collected pre- and mid-operation were positive for Listeria, Listeria isolates obtained were highly related (i.e., ≤10 hqSNP differences), suggesting that pre-operation sampling alone may be sufficient and more effective for detecting sites of Listeria persistence. Importantly, our data also showed that only 1/8 plants showed a significant decrease in Listeria prevalence over 1 year, indicating continued challenges with Listeria control in at least some SMDPs. We conclude that options for simplified Listeria EMP programs (e.g., with a focus on pre-operation sampling, which allows for more rapid identification of likely persistence sites) may be valuable for improved Listeria control in SMDPs.

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Associations between Listeria monocytogenes genomic characteristics and adhesion to polystyrene at 8 °C

Cited by 14 publications

References 36 publications

Atypical Serogroup IVb-v1 of Listeria monocytogenes Assigned to New ST2801, Widely Spread and Persistent in the Environment of a Pork-Meat Producing Plant of Central Italy

Atypical Serogroup IVb-v1 of Listeria monocytogenes Assigned to New ST2801, Widely Spread and Persistent in the Environment of a Pork-Meat Producing Plant of Central Italy

Pervasive Listeria monocytogenes Is Common in the Norwegian Food System and Is Associated with Increased Prevalence of Stress Survival and Resistance Determinants

Intensive environmental sampling and whole genome sequence-based characterization ofListeriain small and medium sized dairy plants reveal opportunities for simplified and size-appropriate environmental monitoring strategies

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