Associations between polymorphisms in the myostatin, α&lt;sup&gt;A&lt;/sup&gt;-globin and lactate dehydrogenase B genes and racing performance in homing pigeons

Dybus, Andrzej; Proskura, Witold Stanisław; Pawlina, E.; Nowak, Błażej

doi:10.17221/149/2017-vetmed

Cited by 9 publications

(15 citation statements)

References 18 publications

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“…The LF-A1 factor occurring in TT individuals may effect on the expression of the pigeon α A -globin gene, however, there is no evidence at the current stage of research. Recently, a very low frequency of allele T (0.077) and only CT genotype detected (no TT individuals) in the group of homing pigeons was reported [Dybus et al 2018].…”

Section: Resultsmentioning

confidence: 99%

“…There are many tools for selection, but the most common types of markers in genomes are SNPs [Goldstein andCavalleri 2005, Jacob et al 2018], nowadays, genetic markers have been used to predict racing perform-ance in pigeons [Proskura et al 2014, Proskura et al 2015, Proskura et al 2017, Ramadan et al 2018, Dybus et al 2018. Recently, in whole-genome study, the CASK gene was marked as an important genetic factor for fast flight, long endurance, and accurate navigation was revealed [Gazda et al 2018].…”

Section: Introductionmentioning

confidence: 99%

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Association of alpha-a globin gene polymorphism with its expression level in racing pigeons

Jędrzejczak-Silicka¹,

Dudaniec²,

Dybus³

2019

Acta Sci. Pol. Zootech.

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The objective of the study was to investigate possible effect of the polymorphism in pigeon α A-globin (AGLOB) gene on its expression level. The g.5768C>T substitution located in 3 flanking region of the gene was analyzed. PCR-RFLP (AvaII) method was used to identify the SNP. The gene expression analyses were performed using qPCR. The investigation indicated a difference but not significant in the α A-globin gene expression levels between pigeons carrying different g.5768C>T genotypes. The AGLOB CC genotype was associated with 2.46-fold higher expression level of pigeon α A-globin gene compared to AGLOB TT genotype. The lower expression of α A-globin gene in AGLOB TT pigeons may be caused by the presence of cis-acting regulatory elements in the analysed gene region.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Association of alpha-a globin gene polymorphism with its expression level in racing pigeons

Jędrzejczak-Silicka¹,

Dudaniec²,

Dybus³

2019

Acta Sci. Pol. Zootech.

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“…With the growing popularity of pigeon racing as a lucrative sport, molecular approaches to the identification of genetic markers, such as SNPs, important to racing and homing phenotypes have been explored in the past [ 1 , 2 , 3 , 5 , 6 , 7 , 8 , 12 , 13 , 17 , 26 , 42 ]. The results of this study demonstrated the application of KASP as a method to detect SNPs previously identified to be associated with the racing traits of pigeons.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, higher overall capacity in terms of endurance and recovery, or speed, velocity, and stress resistance are preferred traits [ 2 , 3 , 6 , 8 , 9 , 10 , 11 ]. Besides health management programs, including the appropriate choice of feeds, medical supplements, and physical conditioning regimens to improve pigeons’ performance in competitions, the identification of genetic markers for valuable traits associated with racing performance has also been reported and explored towards expediting success in selective breeding [ 1 , 6 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. The determination of single nucleotide polymorphisms (SNPs) that seem to cause phenotypic differences is one of the most commonly used approaches to identify potentially essential genetic markers [ 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of KASP Assays for the Genotyping of Racing Performance-Associated Single Nucleotide Polymorphisms in Pigeons

Chang

Silva

Huang

et al. 2021

Genes

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Pigeon racing’s recent upturn in popularity can be attributed in part to the huge prize money involved in these competitions. As such, methods to select pigeons with desirable genetic characteristics for racing or for selective breeding have also been gaining more interest. Polymerase chain reaction—restriction fragment length polymorphism (PCR-RFLP) for genotyping-specific genes is one of the most commonly used molecular techniques, which can be costly, laborious and time consuming. The present study reports the development of an alternative genotyping method that employs Kompetitive Allele Specific Polymerase Chain Reaction (KASP) technology with specifically designed primers to detect previously reported racing performance-associated polymorphisms within the LDHA, MTYCB, and DRD4 genes. To validate, KASP assays and PCR-RFLP assays results from 107 samples genotyped for each of the genes were compared and the results showed perfect (100%) agreement of both methods. The developed KASP assays present an alternative rapid, reliable, and cost-effective method to identify polymorphisms in pigeons.

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“…There are many factors affecting these traits [21], so searching for the appropriate genetic markers is crucial for this purpose. The most common types of markers in genomes are SNPs [22,23], and some of them have been used to predict racing performance in pigeons [20,21,[24][25][26].…”

Section: Short Communicationsmentioning

confidence: 99%

Association of Sequence Variants in the CKM (Creatine Kinase, M-Type) Gene with Racing Performance of Homing Pigeons

Dybus

Proskura

et al. 2020

Russ J Genet

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The aim of the study was to analyze the associations between SNPs in the creatine kinase gene (CKM) and racing performance of homing pigeons. A 468-base pairs fragment of the CKM gene (a part of exon 11 and 3'UTR) was amplified. Five PCR products were sequenced. Two substitutions in the amplified region were observed (g.588268 T>C and g.588285 T>C). PCR-RFLP method was used to genotype 123 racing pigeons (CKM/Hpy188I and CKM/BsrDI) for the further association analysis. The frequencies of genotypes analyzed were: CKM/Hpy188I CC -0.008, CKM/Hpy188I CT -0.057, CKM/Hpy188I TT -0.935 and CKM/BsrDI CC -0.008, CKM/BsrDI CT -0.016, CKM/BsrDI TT -0.976. The effect of one SNP studied on racing performance of pigeons was statistically significant. The largest difference in ace points (APs) was found for CKM/BsrDI (g.588285 T>C). Individuals with the CC and CT genotypes had lower racing performance than TT ones. The differences between CC and TT pigeons were statistically significant (p = 0.0391). Thus, racing pigeons with the most common genotypes (TT) had better racing performance than individuals with minor allele in genotype. However, the observation of an individual with CC genotype in the CKM/Hpy188I with the highest APs in two seasons studied complicates the final conclusion. Based on the obtained results, it may be concluded that two SNPs in the CKM detected in this study were not the major determinant of racing performance in sport pigeons.

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Associations between polymorphisms in the myostatin, α<sup>A</sup>-globin and lactate dehydrogenase B genes and racing performance in homing pigeons

Cited by 9 publications

References 18 publications

Association of alpha-a globin gene polymorphism with its expression level in racing pigeons

Association of alpha-a globin gene polymorphism with its expression level in racing pigeons

Development and Validation of KASP Assays for the Genotyping of Racing Performance-Associated Single Nucleotide Polymorphisms in Pigeons

Association of Sequence Variants in the CKM (Creatine Kinase, M-Type) Gene with Racing Performance of Homing Pigeons

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