Spending a lot of time sitting has been linked to more depressive symptoms and spending a lot of time engaged in screen-based sitting has been linked to greater likelihood of having mental disorders and poorer psychological distress. The purpose of this study was to examine whether overall sitting time and time spent sitting in different contexts was associated with depression, anxiety, or stress symptoms. Sitting time (time spent sitting on typical work-and non-work days while engaged in leisure activities, working, using a computer, watching television, and in transport) and symptom severity of depression, anxiety and stress were self-reported in a cross-sectional online survey in 2012 by Australian adults (N=1,104, 55% female, M age=58 years). Associations were examined using negative binomial regression analyses accounting for the covariates of physical activity, sex, age, income, education, and presence of chronic conditions. Overall sitting time was significantly associated with more severe depression (b=0.01, 95% CI= 0.00 -0.02) and anxiety (b=0.03, 95% CI= 0.02 -0.04) but not stress (b=0.01, 95% CI= -0.00 -0.02) symptoms. Time spent sitting while at a computer was associated with more severe depression (b=0.04, 95% CI= 0.01 -0.07) and anxiety (b=0.03, 95% CI= 0.00 -0.06) symptoms, and time spent sitting while in transport was associated with more severe anxiety (b=0.09, 95% CI= 0.05 -0.13) and stress (b=0.05, 95% CI= 0.02 -0.08) symptoms. Limiting overall sitting time and time spent sitting while at a computer or in transport could be a potential strategy to improve mental health. Evidence is emerging that more sitting time is associated with depressive symptoms or likelihood of having mental disorders (Arredondo et al., 2013; Balboa-Castillo, León-Muñoz, Graciani, Rodríguez-Artalejo, & Guallar-Castillón, 2011;Hamer, Stamatakis, & Mishra, 2010;Peeters, Burton, & Brown, 2012;Sanchez-Villegas et al., 2008;Teychenne, Ball, & Salmon, 2010a, 2010bvan Uffelen et al., 2013), but no studies have tested how sitting time is related to anxiety or stress symptoms. Anxiety and stress are important aspects of mental health to investigate, given that more than 75% of people report experiencing some stress or anxiety symptoms (Crawford & Henry, 2003), and between 5-10% of the population in developed countries have severe depression, anxiety, and stress-related disorders (Demyttenare et al., 2004). Even mild symptoms of anxiety and stress can be highly debilitating and contribute considerably to burden of disease (S. Cohen & Williamson, 1991; Kalia, 2002; Kiecolt-Glaser, McGuire, Robles, & Glaser, 2002; Pietrzak et al., 2012;Rai et al., 2012), so it is important to determine whether sitting time is linked to anxiety and stress, as well as to depression symptoms. These studies suggest that more time spent sitting overall (i.e., across multiple sitting activities) is associated with more severe depressive symptoms; however it remains unclear whether overall sitting time is associated with severity of anxiety or s...