Associations between toxic metals in follicular fluid and in vitro fertilization (IVF) outcomes

Bloom, Michael S.; Kim, Keewan; Kruger, Pamela C.; Parsons, Patrick J.; Arnason, John G.; Steuerwald, Amy J.; Fujimoto, Victor Y.

doi:10.1007/s10815-012-9882-z

Cited by 39 publications

(31 citation statements)

References 45 publications

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“…Further, this may account for unexpected observations, possibly reflecting chance. Second, we collected FF from only a single follicle, and so previously reported follicle-to-follicle variability within woman may bias some of our results towards the null hypothesis [17]. Additionally, we did not adjust for follicle diameter, which may impact FF trace element concentrations [50].…”

Section: Discussionmentioning

confidence: 99%

“…Participant recruitment and clinical protocols were previously described in detail [16,17]. Briefly, between September 1, 2007 andAugust 31, 2008, 58 women completing a first IVF cycle at the University of California San Francisco (USCF) Center for Reproductive Health were enrolled in a prospective study to assess the impact of trace element exposures on IVF endpoints.…”

Section: Sample Selection and Clinical Protocolmentioning

confidence: 99%

“…Biospecimen collection was described previously [16,17]. Briefly, FF was collected during oocyte retrieval by ultrasound-guided transvaginal fine needle aspiration.…”

Section: Exposure Assessmentmentioning

confidence: 99%

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Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

Ingle

Bloom

Parsons

et al. 2016

J Assist Reprod Genet

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Purpose A hypothesis-generating pilot study exploring associations between essential trace elements measured in follicular fluid (FF) and urine and in vitro fertilization (IVF) endpoints. Methods We recruited 58 women undergoing IVF between 2007 and 2008, and measured cobalt, chromium, copper, manganese, molybdenum, and zinc in FF (n = 46) and urine (n = 45) by inductively coupled plasma mass spectrometry (ICP-MS). We used multivariable regression models to assess the impact of FF and urine trace elements on IVF outcomes, adjusted for age, body mass index, race, and cigarette smoking.Results Trace elements were mostly present at lower concentrations in FF than in urine. The average number of oocytes retrieved was positively associated with higher urine cobalt, chromium, copper, and molybdenum concentrations. FF chromium and manganese were negatively associated with the proportion of mature oocytes, yet urine manganese had a positive association. FF zinc was inversely associated with average oocyte fertilization. Urine trace elements were significant positive predictors for the total number of embryos generated. FF copper predicted lower embryo fragmentation while urine copper was associated with higher embryo cell number and urine manganese with higher embryo fragmentation. No associations were detected for implantation, pregnancy, or live birth. Conclusions Our results suggest the importance of trace elements in both FF and urine for intermediate, although not necessarily clinical, IVF endpoints. The results differed using FF or urine biomarkers of exposure, which may have implications for the design of clinical and epidemiologic investigations. These initial findings will form the basis of a more definitive future study.

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Section: Discussionmentioning

confidence: 99%

Section: Sample Selection and Clinical Protocolmentioning

confidence: 99%

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Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

Ingle

Bloom

Parsons

et al. 2016

J Assist Reprod Genet

Self Cite

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“…294 Bloom et al quantified Hg, Cd and Pb in the follicular fluid surrounding the oocyte and in blood. 295 While they found a correlation for Hg and Cd concentrations in the two fluids, this was not the case for Pb. Interestingly, the Cd concentration correlated positively with fertilisation and even stronger with pregnancy.…”

Section: Elemental Analyses Of Body Tissues and Body Fluidsmentioning

confidence: 93%

Inductively Coupled Plasma Mass Spectrometry

Jakubowski

Horsky

Roos

et al. 2014

Sector Field Mass Spectrometry for Elemental and Isotopic Analysis

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ICP-MS is based on the formation of (preferentially monovalent positively) charged atomic ions in an inductively coupled Ar plasma at almost 10 000 K. The ions formed are transferred from the plasma source at ambient pressure into a mass separator operated at high vacuum via a set of cones. The ions are separated according to their mass/charge ratio in the mass separator (quadrupole, magnetic sector field or time-of-flight mass separator). In most cases, the ions are detected using a secondary electron multiplier; in some set-ups (also) a Faraday cup can be used. Single-collector (scanning mass spectrometer usually used for quantitative elemental analysis) or multicollector (static operation of mass spectrometer for precise isotope ratio analysis) configurations can be found (Figures 12.1 and 12.2).

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“…Some authors have identified associations between trace amounts of Pb and Cd in follicular fluids from a single follicle and oocyte fertilization. An inverse association has been detected between Pb in follicular fluids and fertilization, although the association is positive for Cd (Bloom et al, 2012); furthermore, embryo fragmentation is reduced by higher blood Hg (Bloom et al, 2011).…”

Section: Direct Effects On the Ovary And Ovarian Productionmentioning

confidence: 99%