Associations of CYP1A2 Polymorphisms with the Risk Haplotypes in Lung Cancer in the Slovak Population

Abstract

Phase I enzymes, including cytochrome P450, family 1, subfamily A, and polypeptide 2 (CYP1A2), are involved in the activation of carcinogens to reactive intermediates that are capable of binding covalently to DNA to form DNA adducts, potentially initiating the carcinogenic process. The aim of present study was to investigate the association of CYP1A2 gene polymorphisms and haplotypes with lung cancer risk. A case-control study was carried out on 105 lung cancer patients and 189 controls. To investigate three CYP1A2 polymorphisms: rs2472299, rs2470890, rs11072508 we used a high resolution melting analysis. We found significant allele associations (rs2470890 and rs2422299) with lung cancer risk. We searched for meaningful associations for all variants in the dominant, recessive, and additive genetic models. Genotype associations in the recessive model were of marginal significance for the same single nucleotide polymorphisms. A haplotype analysis included five variants with the frequency higher than 1 %. The haplotype "acc", present with the highest frequency, was associated with increased lung cancer risk (38.7 % vs. 31.5 %; OR 1.38; 95 %CI 0.95-2.01). On the contrary, rare haplotype "gtc" was significantly associated with decreased lung cancer risk in the Slovak population. In conclusion, the present study identified the risk alleles and haploid genotype associations of the CYP1A2 gene in lung cancer.

Polymorphisms in the gene encoding CYP1A2 influence prostate cancer risk and progression

“…Only one study has investigated the association of these three CYP1A2 polymorphisms and haplotypes with cancer risk. Matakova et al ( 31 ) studied the risk of lung cancer in a case-control study of 105 patients with lung cancer and 189 controls. The study reported a significant association of the CYP1A2 rs2470890 and rs2422299 polymorphisms with lung cancer risk.…”

“…Three tagged SNPs, capturing common variability of the CYP1A2 gene, were adopted according to the approach described by Matakova et al ( 31 ). The common variability given by the presence of polymorphic sites with minor allele frequency >10% was studied.…”