Associations of genetic variants of miRNA coding regions with pulmonary tuberculosis risk in China

Abstract

Tuberculosis (TB) is the leading cause of death caused by single pathogenic microorganism of mycobacterium tuberculosis (MTB). The study aims to explore the associations of microRNA (miRNA) single nucleotide polymorphisms (SNPs) with pulmonary TB (PTB) risk. A population-based case-control study was conducted, and 168 newly diagnosed smear-positive PTB cases and 251 non-TB controls were recruited. SNPs located within miR-27a (rs895819), miR-423 (rs6505162), miR-196a-2 (rs11614913), miR-146a (rs2910164), miR-618 (rs2682818) were selected and MassARRAY® MALDI-TOF System was employed for genotyping. SPSS19.0 was adopted for statistical analysis, non-conditional logistic regression was performed. Odds ratios (ORs) and 95%confidence intervals (95%CIs) were computed to estimate the associations.Associations of haplotypes with PTB risk was performed with online tool. Rs895819 CT/CC genotype was associated with reduced PTB risk among female population (OR= 0.45, 95%CI: 0.23-0.98), p=0.045. Haplotypes (Combined with rs895819, rs2682818, rs2910164, rs6505162 and rs11614913) TCCCT, TAGCC, CCCCC, CCGCT and TCGAT were associated with reduced

Associations of genetic variants within TYK2 with pulmonary tuberculosis among Chinese population

“…Genotyping was performed with MassARRAY® MALDI‐TOF System (US, Sequenom, Inc.), and three primers for each SNP were designed, as shown in Table 1. Four main steps included in the protocol adopted in our present study were described as follows, and details of the protocol have been presented in our previous study (Mingwu Zhang et al., 2021). Polymerase chain reaction (PCR)PCR reactions were performed and each 5 μL PCR volume containing 1 μL (30 ng) DNA template. SPA reactionTwo microliter of SPA reaction buffer was added into each PCR well, incubating at 37°C for 40 min, at 85°C for 5 min, and at 4°C forever. Single base extensionTwo microliter of iPlex reaction reagent was added into each PCR well and the reactions were performed according to the given parameter. Purification and measurementPurified reaction products were spotted on sample plates.…”