Associations of PCR markers with freezing tolerance and photosynthetic acclimation to cold in winter barley

Rapacz, Marcin; Tyrka, Mirosław; Gut, M.; Mikulski, W.

doi:10.1007/s10681-010-0127-x

Cited by 14 publications

(24 citation statements)

References 31 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The CG marker can thus be used not only for selection of GH types in winter x spring crosses, but also for fast routine selection of frost tolerant genotypes (Akar et al 2009). Similar indications came from the work of Rapacz et al (2010) that found variation in the promoter region of Vrn-H1 (HvBM5) directly connected with freezing tolerance of plants partially de-acclimated in the field.…”

Section: Ld-mas For Freezing Tolerancesupporting

confidence: 74%

Genomics of Low-Temperature Tolerance for an Increased Sustainability of Wheat and Barley Production

Pecchioni

Kosová

Vítámvás

et al. 2013

Genomics of Plant Genetic Resources

View full text Add to dashboard Cite

Stability of high yields in a changing environment becomes the main aim of the future wheat and barley breeding, oriented towards development of frosttolerant winter and facultative cultivars together with careful selection of growth cycle adaptation and drought tolerance. Since low temperature signal influences both the cold acclimation and vernalization processes the interaction between VRN gene expression and freezing tolerance (FT) is discussed. Recent advances in global expression changes driven by cold are reviewed in view of the immense progress in high throughput technological platforms. Different signal transduction pathways in which several transcription factors play an important role regulating the expression of whole sets of genes are presented, including CBF-regulated and CBF-independent hubs. The knowledge acquired from genomics and transcriptome analysis has been then complemented by the description of metabolomics and proteomic approaches to help unraveling the molecular changes that occur under cold stress in the cereal plants. Finally, it is surveyed the great importance of stable and well-characterized genetic resources for future breeding for FT, that could switch from marker-assisted to genomics-assisted selection.

show abstract

Section: Ld-mas For Freezing Tolerancesupporting

confidence: 74%

Genomics of Low-Temperature Tolerance for an Increased Sustainability of Wheat and Barley Production

Pecchioni

Kosová

Vítámvás

et al. 2013

Genomics of Plant Genetic Resources

View full text Add to dashboard Cite

show abstract

“…Associations between FT and markers from the region of Fr‐H2 on chromosome 5H (previously found by Rapacz et al. ) were not important for explaining variation of PA and FT in the P44 and CaP populations.…”

Section: Discussionmentioning

confidence: 54%

“…PCRs were as described previously (Rapacz et al. ). Digestion of selected products with restriction enzymes was carried out in 10 or 15 μl volumes containing 2× concentrated buffer recommended for the respective restriction enzymes (Fermentas, Vilnius, Lithuania) and 10 or 5 μl of PCR product, respectively.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Quantitative trait loci mapping of freezing tolerance and photosynthetic acclimation to cold in winter two‐ and six‐rowed barley

et al. 2015

View full text Add to dashboard Cite

Photoacclimation (PA) and freezing tolerance (FT) have been identified as closely related traits, due to common mechanisms of environmental control. In this study, diversity array technology (DArT) was used for identification of the quantitative trait loci (QTL) of FT and PA in winter barley. Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were subsequently used to saturate QTL regions. Two F2 mapping populations were created, for two‐rowed (P44) and six‐rowed barley (CaP). Different regions of the genome were responsible for differences in traits between parents in these two populations. Eleven QTLs were identified in the P44 population, including five typical for FT and PA, on chromosomes 2H, 3H and 7H. In the CaP population, only one QTL connected with PA and 10 connected with FT were found on all chromosomes except 2H. Our results demonstrate that different sets of markers should be applied in marker‐assisted selection for FT in two‐ and six‐rowed barley, as several loci determine FT at the level of biparental crosses.

show abstract

“…A less simple genetic model was obtained from controlled environment experiments, where nine freezing tolerance QTLs, distributed across four of the seven barley chromosomes, were identified (Tuberosa et al 1997). More recently, the loci Fr-H1 and Fr-H2, both involved in the determination of freezing tolerance, were mapped to the long arm of barley chromosome 5H (Skinner et al 2006;Francia et al 2007), and DNA-based genetic markers for these loci have been developed since then (Akar et al 2009;Rapacz et al 2010).…”

mentioning

confidence: 99%

Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley

Holková¹,

Mikulková²,

Hrstková³

et al. 2010

CZECH J GENET PLANT

View full text Add to dashboard Cite

Abstract:The sequences of the closely linked barley dehydrin genes dhn4 and dhn7 are both known to vary allelically. Here we associated allelic constitution at dhn4/7 with frost sensitivity across a panel of 30 diverse barley varieties. The combined presence of a 6 bp insertion in exon1 of dhn4 and a 30 bp deletion in exon1 of dhn7 was restricted to six-rowed winter and intermediate varieties characterised by relatively higher frost tolerance (12 genotypes; LT 50 from -14.2°C to -15.6°C). The alternative combination was present with one exception (sixrowed winter variety Alissa) only in spring and two-rowed winter varieties (17 genotypes; LT 50 from -10.0°C to -14.3°C). The genetic linkage between dhn4 and dhn7 identified e.g. in Dictoo and Morex varieties was verified by segregation analysis of F 2 plants from a cross between two genotypes carrying different allelic combination of dhn4 and dhn7 genes (two-rowed spring variety Akcent × six-rowed winter variety Okal). The potential of the former allelic combination as a marker for enhanced frost tolerance was tested in a sample of F 5 derivatives of a cross between the two-rowed winter type variety Monaco (Akcent allele combination) and the six-rowed winter type variety Okal. Plants with the Okal allele combination showed significantly higher frost tolerance than those with the alternative growth habit. The effect of ear type on frost tolerance was insignificant.

show abstract

Associations of PCR markers with freezing tolerance and photosynthetic acclimation to cold in winter barley

Cited by 14 publications

References 31 publications

Genomics of Low-Temperature Tolerance for an Increased Sustainability of Wheat and Barley Production

Genomics of Low-Temperature Tolerance for an Increased Sustainability of Wheat and Barley Production

Quantitative trait loci mapping of freezing tolerance and photosynthetic acclimation to cold in winter two‐ and six‐rowed barley

Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley

Contact Info

Product

Resources

About