2018
DOI: 10.1371/journal.pone.0197320
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Associative nitrogen fixation (ANF) in switchgrass (Panicum virgatum) across a nitrogen input gradient

Abstract: Associative N fixation (ANF), the process by which dinitrogen gas is converted to ammonia by bacteria in casual association with plants, has not been well-studied in temperate ecosystems. We examined the ANF potential of switchgrass (Panicum virgatum L.), a North American prairie grass whose productivity is often unresponsive to N fertilizer addition, via separate short-term 15N2 incubations of rhizosphere soils and excised roots four times during the growing season. Measurements occurred along N fertilization… Show more

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Cited by 88 publications
(67 citation statements)
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“…FLNF has also been directly observed in association with switchgrass in our own work ( Fig. 4) and by others (14,15).…”
Section: Figsupporting
confidence: 80%
See 1 more Smart Citation
“…FLNF has also been directly observed in association with switchgrass in our own work ( Fig. 4) and by others (14,15).…”
Section: Figsupporting
confidence: 80%
“…As diazotrophs fix N, the 15 N label is incorporated into the soil and can be measured to determine FLNF rates (35). A reference sample which provides 15 N content in a given sample prior to 15 N 2 fixation is required to obtain FLNF rates. 15 N isotope enrichment is a direct measure of FLNF rates and is, therefore, more accurate than acetylene reduction.…”
Section: Figmentioning
confidence: 99%
“…, ). Roley et al., () found that the majority of N was fixed in the soil (not directly in the plant) post‐senescence, perhaps when the microbial community had a boost of carbon exudates from the senescing switchgrass plants. AMF hyphae are known to help retain N pools and prevent N leaching (De Vries and Bardgett ), therefore the increased extra‐radical hyphae we found in the low‐N‐addition treatments may play a primary role in immobilizing and retaining N fixed from ANF.…”
Section: Discussionmentioning
confidence: 99%
“…This method also assumes that the reference plant (rye) was not affected by free living N 2 fixation in the rhizosphere, a topic of renewed attention in agroecosystem research on perennial grasses (Roley et al. , Smercina et al. ).…”
Section: Discussionmentioning
confidence: 99%
“…Consequently, applying one B value across sites, to account for isotopic fractionation, may not fully meet the assumptions of the natural abundance method. This method also assumes that the reference plant (rye) was not affected by free living N 2 fixation in the rhizosphere, a topic of renewed attention in agroecosystem research on perennial grasses (Roley et al 2018, Smercina et al 2019. However, given the experimental design, in which the reference plants were in small plots nested within a larger field inoculated with rhizobia, and given that the farm management histories often included legumes in crop rotation, this assumption is likely valid.…”
Section: Changes In Labile Organic Matter Pools With a Legumegrass MImentioning
confidence: 99%