Astaxanthin decreases the growth-inhibitory dose of cytarabine and inflammatory response in the acute lymphoblastic leukemia cell line NALM-6

Rastgar, Amirhossein; Sayadi, Mahtab; Sarab, Gholamreza Anani; Sajjadi, Seyed Mehdi

doi:10.1007/s11033-022-07452-8

Cited by 7 publications

(1 citation statement)

References 32 publications

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“…of cancer new cases with a mortality rate of 3.1% of all cancer-related deaths [3]. Acute lymphoblastic leukemia (ALL) is a heterogeneous hematological disorder caused by a disturbance in the proliferation and differentiation of immature lymphoid cells in the bone marrow, peripheral blood, and other parts outside the bone marrow [4,5]. B cell-derived ALLs by themself, account for 25% of all pediatric tumors and 80% of pediatric ALL.…”

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confidence: 99%

The Impact of Exosomes Derived from B-Cell Acute Lymphoblastic Leukemia as a Growth Factor on Bone Marrow Mesenchymal Stromal Cells

Amirpour,

Kuhestani,

Kheyrandish

et al. 2024

Preprint

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Background The incidence of various types of cancers, including leukemia, is on the rise and many challenges in both drug resistance and complications related to chemotherapy appeared. Recently, the development and application of extracellular vesicles (EV) such as exosomes in the management of cancers, especially leukemia, holds great significance. Aim In this article, we extracted exosomes from NALM6 cells and assessed their regulatory effects on proliferation and apoptosis in mesenchymal stem cells (MSCs). Method We first verified the exosomes using various techniques, including flow cytometry, transient electron microscopy, dynamic light scattering (DLS), and BCA protein assay. Then MTT analysis and flowcytometry (apoptosis and cell cycle assay) besides gene expressions were employed to determine the state of MSC proliferations. Result The results indicated that exosome-specific pan markers like CD9, CD63, and CD81 were present. Through DLS, we found out that the mean size of the exosomes was 89.68 nm. The protein content was determined to be 956.292 µg/ml. Analysis of MTT, flow cytometry (cell cycle and apoptosis assay), and RT-qPCR showed that in the dose of 50 µg/ml the proliferation of MSCs was increased significantly (p-value < 0.05). Conclusion All these data showed that exosomes use several signaling pathways to increase the MSCs’ proliferation and drug resistance, ultimately leading to high mortalities and morbidities of acute lymphoblastic leukemia.

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confidence: 99%