Astaxanthin improves preserved semen quality at 17°C by enhancing sperm antioxidant and motility properties

Hu, Yamei; Hu, Jianhong; Pang, Wei; Yang, Gongshe; Chu, Guiyan

doi:10.1111/rda.14190

Cited by 4 publications

(2 citation statements)

References 45 publications

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“…When the accumulation of reactive oxygen molecules reaches a certain level, the antioxidant system of the sperm is disrupted, resulting in lipid peroxidation damage and subsequent irreversible negative effects on sperm vitality, viability, and quality [ 7 ]. Studies have shown that exogenous antioxidant substances can be added to semen diluents to reduce oxidative stress, limit the damage caused by ROS, extend sperm preservation time, and maintain quality after preservation [ 8 , 9 ]. Currently, the exogenous antioxidants used in semen preservation can be mainly divided into antioxidant enzymes, vitamins, amino acids, and plant extracts [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Preservative Effects of Curcumin on Semen of Hu Sheep

Ji,

Wei,

Fan

et al. 2024

Animals

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Reactive oxygen species (ROS) are important factors that lead to a decline in sperm quality during semen preservation. Excessive ROS accumulation disrupts the balance of the antioxidant system in sperm and causes lipid oxidative damage, destroying its structure and function. Curcumin is a natural plant extract that neutralizes ROS and enhances the function of endogenous antioxidant enzymes. The effect of curcumin on the preservation of sheep semen has not been reported. This study aims to determine the effects of curcumin on refrigerated sperm (4 °C) and analyze the effects of curcumin on sperm metabolism from a Chinese native sheep (Hu sheep). The results showed that adding curcumin significantly improved (p < 0.05) the viability of refrigerated sperm at an optimal concentration of 20 µmol/L, and the plasma membrane and acrosome integrity in semen were significantly improved (p < 0.05). Adding curcumin to refrigerated semen significantly increased (p < 0.05) the levels of antioxidant enzymes (T-AOC, CAT, and SOD) and significantly decreased (p < 0.05) ROS production. A total of 13,796 metabolites in sperm and 20,581 metabolites in negative groups and curcumin-supplemented groups were identified using liquid chromatography–mass spectrometry. The proportion of lipids and lipid-like molecules among all metabolites in the sperm was the highest, regardless of treatment. We identified 50 differentially expressed metabolites (DEMs) in sperm between the negative control and curcumin-treated groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEMs were mainly enriched in the calcium signaling pathway, phospholipase D signaling pathway, sphingolipid metabolism, steroid hormone biosynthesis, 2-oxocarboxylic acid metabolism, and other metabolic pathways. The findings indicate that the addition of an appropriate concentration (20 µm/L) of curcumin to sheep semen can effectively suppress reactive oxygen species (ROS) production and extend the duration of cryopreservation (4 °C) by modulating the expression of sphingosine-1-phosphate, dehydroepiandrosterone sulfate, phytosphingosine, and other metabolites of semen. This discovery offers a novel approach to enhancing the cryogenic preservation of sheep semen.

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Section: Introductionmentioning

confidence: 99%

Preservative Effects of Curcumin on Semen of Hu Sheep

Ji,

Wei,

Fan

et al. 2024

Animals

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“…Besides, mammalian spermatozoa are particularly sensitive to oxidative stress which result in collateral damage to sperm plasma membrane proteins and lipids (8). It is a proven fact that during storage and transport the impact of oxidative stress on the sperm cell increases (9).…”

Section: Introductionmentioning

confidence: 99%

Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams.

Jurado-Campos

Soria-Meneses

Arenas-Moreira

et al. 2023

Preprint

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Background Artificial insemination (AI) is a routine breeding technology in animal reproduction. Nevertheless, the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI. In this sense, nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI, which could be used as delivery vehicles. In this work, we explored the feasibility of nanoemulsions of vitamin E (NE) for improving sperm quality during transport. Results With the aim of evaluating this proposal, ejaculates of seven mature rams of Manchega breed were collected by artificial vagina and extended to 60 x 106 spz/mL in Andromed®. Samples containing control and NE (12 mM) with and without exogenous oxidative stress (100 µM Fe2+/ascorbate) were stored at 22 and 15 ºC and motility (CASA), viability (YO-PRO/IP), acrosomal integrity (PNA-FITC/IP), mitochondrial membrane potential (Mitotracker Deep Red 633), lipoperoxidation (C11 BODIPY 581/591), intracellular reactive oxygen species (ROS) production and DNA status (SCSA®) monitored during 96 hours. Our results show that NE could be used to maintain ram spermatozoa during transport at 15 ºC and 22 ºC for up to 96 h, with no appreciable loss of kinematic and physiological characteristics of freshly collected samples. Conclusions The storage of ram spermatozoa in liquid form for 2-5 days with vitamin E nanoemulsions may lead more flexibility to breeders in AI programs. In view of the potential and high versatility of these nanodevices, further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination.

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Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams

Jurado-Campos,

Soria-Meneses,

Arenas-Moreira

et al. 2023

J Animal Sci Biotechnol

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Background Artificial insemination (AI) is a routine breeding technology in animal reproduction. Nevertheless, the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI. In this sense, nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI, which could be used as delivery vehicles. In this work, we explored the feasibility of vitamin E nanoemulsion (NE) for improving sperm quality during transport. Results With the aim of evaluating this proposal, ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60 × 106 spz/mL in Andromed®. Samples containing control and NE (12 mmol/L) with and without exogenous oxidative stress (100 µmol/L Fe2+/ascorbate) were stored at 22 and 15 ºC and motility (CASA), viability (YO-PRO/PI), acrosomal integrity (PNA-FITC/PI), mitochondrial membrane potential (Mitotracker Deep Red 633), lipoperoxidation (C11 BODIPY 581/591), intracellular reactive oxygen species (ROS) production and DNA status (SCSA®) monitored during 96 h. Our results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22 ºC for up to 96 h, with no appreciable loss of kinematic and physiological characteristics of freshly collected samples. Conclusions The storage of ram spermatozoa in liquid form for 2–5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI programs. In view of the potential and high versatility of these nanodevices, further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination. Graphical Abstract

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Astaxanthin improves preserved semen quality at 17°C by enhancing sperm antioxidant and motility properties

Cited by 4 publications

References 45 publications

Preservative Effects of Curcumin on Semen of Hu Sheep

Preservative Effects of Curcumin on Semen of Hu Sheep

Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams.

Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams

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