Astaxanthin reduces MMP expressions, suppresses cancer cell migrations, and triggers apoptotic caspases of in vitro and in vivo models in melanoma

Chen, Yen‐Ting; Kao, Chien-Jen; Huang, Hsin‐Yi; Huang, Shiying; Chen, Chung‐Yi; Lin, Yun‐Sheng; Wen, Zhi‐Hong; Wang, Hui‐Min David

doi:10.1016/j.jff.2017.01.005

Cited by 59 publications

(55 citation statements)

References 45 publications

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“…Phytochemicals from marine sources have gained increasing importance due to their innumerable health benefits. Astaxanthin (AXT), a ketocarotenoid red pigment predominantly found in green microalgae Heamatococcus pluvialis , yeast Xanthophyllomyces dendrorhous , and in marine organisms such as salmon, is a potent antioxidant . Astaxanthin has also been reported to exhibit anti‐inflammatory and anti‐proliferative properties .…”

Section: Introductionmentioning

confidence: 99%

Astaxanthin inhibits hallmarks of cancer by targeting the PI3K/NF‐κΒ/STAT3 signalling axis in oral squamous cell carcinoma models

et al. 2019

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Aberrant activation of the PI3K/Akt signalling pathway, a major driving force of diverse cellular processes has been implicated in tumour development and progression. Here, we report that astaxanthin (AXT), a potent antioxidant ketocarotenoid prevents cancer hallmarks by inhibiting PI3K/Akt and the associated downstream NF‐κB and STAT‐3 signalling pathways in SCC131 and SCC4 oral cancer cells as well as in the hamster buccal pouch carcinogenesis model. Using small molecule inhibitors of NF‐κB, STAT‐3 and PI3K and by overexpression of PI3K, we provide evidence to show that AXT inhibits NF‐κB and STAT‐3 signalling and cancer hallmarks by restraining the kinase activity of PI3K/Akt. Additionally, AXT downregulated the noncoding RNAs (ncRNAs), miR‐21 and HOTAIR that influence PI3K/Akt signalling emphasising its modulatory effects on epigenetic regulation. Ethyl cellulose‐based AXT nanoparticles showed greater chemotherapeutic efficacy in the hamster oral carcinogenesis model compared to native AXT. We suggest that AXT prevents cell proliferation, apoptosis evasion, invasion and angiogenesis by intercepting the crosstalk between the PI3K/Akt, NF‐κB and STAT‐3 signalling circuits both in vitro and in vivo. Astaxanthin that abrogates the PI3K/Akt signalling axis, a central hub that orchestrates acquisition of cancer hallmarks is a promising candidate for anticancer drug development.

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Section: Introductionmentioning

confidence: 99%

Astaxanthin inhibits hallmarks of cancer by targeting the PI3K/NF‐κΒ/STAT3 signalling axis in oral squamous cell carcinoma models

et al. 2019

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“…Astaxanthin is one of the most important carotenoids with a very high antioxidant activity . A growing body of evidence indicates that astaxanthin may contribute to the prevention of some human health disorders, such as cancer, cardiovascular disease, cataracts and macular degeneration . Its consumption has been associated with various benefits for human health, including protection against ultraviolet (UV)‐light photo‐oxidation and anti‐inflammatory and anti‐carcinogenic effects, as well as enhancement of the immune response and liver and heart function .…”

Section: Introductionmentioning

confidence: 99%

Thermal stability and oral absorbability of astaxanthin esters from Haematococcus pluvialis in Balb/c mice

Qingxin

Yang

et al. 2019

J Sci Food Agric

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BACKGROUND Astaxanthin is used as a functional nutraceutical and pigment in many food products. It is mostly exists in the form of a fatty acid ester in nature. However, no detailed descriptions are available concerning the stability and oral absorbability of astaxanthin esters. In the present study, the thermal stability and absorbability of astaxanthin esters from Haematococcus pluvialis were evaluated in comparison with free‐form astaxanthin. RESULTS The thermal stability of astaxanthin esters was found to be higher than that of free‐form astaxantin. After gavage with astaxanthin esters, only free‐form astaxanthin was detected in the digestive tract wall, blood plasma and liver, indicating that astaxanthin esters must be hydrolyzed to free‐form astaxanthin in the gut before absorption. Furthermore, there was a considerable selective accumulation of different astaxanthin isomers in Balb/c mice, which selectivity decreased in the order: 13‐cis > all‐trans > 9‐cis. Accumulated astaxanthin was mainly distributed in the heart, liver, spleen, muscle and adipose tissue, although significant differences between tissues were observed. CONCLUSION From the present study, it can be concluded that astaxanthin esters had a higher thermal stability and higher bioavailability than free‐form astaxanthin. These results provide important evidence with respect to using astaxanthin esters as bioactive components to replace free‐form astaxanthin in functional food products. © 2019 Society of Chemical Industry

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“…The effects of HSCAE on cell viability were estimated by the MTT method . Cells were cultured for 24 h in a 96‐well plate with a density of 1 × 10, and then cultured for 4 h in a serum‐free medium. The medium was removed and cells were incubated for another 24 h with HSCAE at various concentrations from 0.05 to 10 mg mL −1 , and the final volume was 100 μL.…”

Section: Methodsmentioning

confidence: 99%

“…Overproduction of ROS can induce oxidative stress, resulting in cells failing to maintain normal physiological redox‐regulated functions . Anti‐oxidants are therefore essential for human ability to scavenge free radicals and reduce abnormal physiological degradation …”

Section: Introductionmentioning

confidence: 99%

“…Cellular senescence is the decrease of cell replication ability with time, and often occurs in aged skin. With time, the increased expression of matrix metalloproteinase (MMP), which degrades collagen in ECM, has been shown in senescent fibroblasts . Increasing tissue inhibitor of metalloproteinase (TIMP) prevents collagen degradation by MMP and has a beneficial effect in keeping collagen content in the skin …”

Section: Introductionmentioning

confidence: 99%

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Reversing UVB‐induced photoaging with Hibiscus sabdariffa calyx aqueous extract

Lin

et al. 2019

J Sci Food Agric

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BACKGROUND Hibiscus sabdariffa is commonly used in daily life and its extract is applied widely in food and cosmetics. However, it has not been evaluated for its anti‐aging effects. RESULTS Hibiscus sabdariffa calyx aqueous extract (HSCAE) has shown potential collagenase activity suppression effects, together with tyrosinase activity inhibition, and anti‐oxidation as a free radical scavenger. The current investigation demonstrated that HSCAE was not cytotoxic in skin fibroblasts, and it significantly decreased ultraviolet B (UVB)‐induced reactive oxygen species (ROS) on a flow cytometry assay. Moreover, HSCAE reduced matrix metalloproteinase (MMP) expression, increased tissue inhibition of metalloproteinase (TIMP)‐1 level, and enhanced collagen content by inhibiting collagenase activity. It also blocked mRNA and protein expressions of melanin production pathway key factors, including the microphthalmia‐associated transcription factor (MITF), tyrosinase, tyrosinase‐related protein‐1 (TRP‐1), and dopachrome tautomerase‐2 (TRP‐2). CONCLUSION These results demonstrated, for the first time, the potential of HSCAE as a natural antioxidant with the ability to maintain collagen production and to decrease melanin syntheses under UVB radiation, for anti‐aging effects. © 2019 Society of Chemical Industry

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Astaxanthin reduces MMP expressions, suppresses cancer cell migrations, and triggers apoptotic caspases of in vitro and in vivo models in melanoma

Cited by 59 publications

References 45 publications

Astaxanthin inhibits hallmarks of cancer by targeting the PI3K/NF‐κΒ/STAT3 signalling axis in oral squamous cell carcinoma models

Astaxanthin inhibits hallmarks of cancer by targeting the PI3K/NF‐κΒ/STAT3 signalling axis in oral squamous cell carcinoma models

Thermal stability and oral absorbability of astaxanthin esters from Haematococcus pluvialis in Balb/c mice

Reversing UVB‐induced photoaging with Hibiscus sabdariffa calyx aqueous extract

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