Asticcacaulis benevestitus sp. nov., a psychrotolerant, dimorphic, prosthecate bacterium from tundra wetland soil

Vasilyeva, Leana V.; Omelchenko, Marina V.; Berestovskaya, Yulia Y.; Lysenko, A. M.; Abraham, Wolf‐Rainer; Dedysh, Svetlana N.; Zavarzin, G. A.

doi:10.1099/ijs.0.64122-0

Cited by 39 publications

(21 citation statements)

References 22 publications

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“…It can be assumed that the microbes found on the larvae originate either from the larval microbiota or the environment. Among the less common taxa of the exclusive larva OTUs is the genus Elizabethkingia, that has been isolated from mosquitoes among other places [47] as well as Asticcacaulis, previously isolated from tundra wetland soil [48] in support of the above assumption.…”

Section: Insects and Parasitesmentioning

confidence: 75%

Microbiota in foods from Inuit traditional hunting

et al. 2020

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The foods we eat contain microorganisms that we ingest alongside the food. Industrialized food systems offer great advantages from a safety point of view, but have also been accused of depleting the diversity of the human microbiota with negative implications for human health. In contrast, artisanal traditional foods are potential sources of a diverse food microbiota. Traditional foods of the Greenlandic Inuit are comprised of animal-sourced foods prepared in the natural environment and are often consumed raw. These foods, some of which are on the verge of extinction, have not previously been microbiologically characterized. We mapped the microbiota of foods stemming from traditional Inuit land-based hunting activities. The foods included in the current study are dried muskox and caribou meat, caribou rumen and intestinal content as well as larval parasites from caribou hides, all traditional Inuit foods. This study shows that traditional drying methods are efficient for limiting microbial growth through desiccation. The results also show the rumen content of the caribou to be a highly diverse source of microbes with potential for degradation of plants. Finally, a number of parasites were shown to be included in the biodiversity of the assessed traditional foods. Taken together, the results map out a diverse source of ingested microbes and parasites that originate from the natural environment. These results have implications for understanding the nature-sourced traditional Inuit diet, which is in contrast to current day diet recommendations as well as modern industrialized food systems.

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Section: Insects and Parasitesmentioning

confidence: 75%

Microbiota in foods from Inuit traditional hunting

et al. 2020

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“…In addition, Devosia species were isolated from greenhouse soil and beach sediments, testing positive for the hydrolysis of esculin, β-galactosidase, β-glucosidase, and N -acetyl-β-glucosaminidase, although unable to degrade carboxymethyl cellulose (CMC) (51, 52). Phenylobacterium and Burkholderia are abundant in forest soils (53) and the genus Asticcaulis was identified among aerobic chemoorganoheterotrophs in tundra wetlands, able to use glucose, sucrose, xylose, maltose, galactose, arabinose, lactose, fructose, rhamnose, and trehalose, among other carbon sources (54). The genus Spirochaeta has some species that are free-living saccharolytic and obligate or facultative anaerobes and were isolated from diverse environments, mainly from extreme aquatic environments (55, 56).…”

Section: Resultsmentioning

confidence: 99%

Multisubstrate Isotope Labeling and Metagenomic Analysis of Active Soil Bacterial Communities

Verastegui

Cheng

Engel

et al. 2014

mBio

119

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Soil microbial diversity represents the largest global reservoir of novel microorganisms and enzymes. In this study, we coupled functional metagenomics and DNA stable-isotope probing (DNA-SIP) using multiple plant-derived carbon substrates and diverse soils to characterize active soil bacterial communities and their glycoside hydrolase genes, which have value for industrial applications. We incubated samples from three disparate Canadian soils (tundra, temperate rainforest, and agricultural) with five native carbon (12C) or stable-isotope-labeled (13C) carbohydrates (glucose, cellobiose, xylose, arabinose, and cellulose). Indicator species analysis revealed high specificity and fidelity for many uncultured and unclassified bacterial taxa in the heavy DNA for all soils and substrates. Among characterized taxa, Actinomycetales (Salinibacterium), Rhizobiales (Devosia), Rhodospirillales (Telmatospirillum), and Caulobacterales (Phenylobacterium and Asticcacaulis) were bacterial indicator species for the heavy substrates and soils tested. Both Actinomycetales and Caulobacterales (Phenylobacterium) were associated with metabolism of cellulose, and Alphaproteobacteria were associated with the metabolism of arabinose; members of the order Rhizobiales were strongly associated with the metabolism of xylose. Annotated metagenomic data suggested diverse glycoside hydrolase gene representation within the pooled heavy DNA. By screening 2,876 cloned fragments derived from the 13C-labeled DNA isolated from soils incubated with cellulose, we demonstrate the power of combining DNA-SIP, multiple-displacement amplification (MDA), and functional metagenomics by efficiently isolating multiple clones with activity on carboxymethyl cellulose and fluorogenic proxy substrates for carbohydrate-active enzymes.

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“…(h) Transmission electron micrographs of A. excentricus grown under phosphate starvation. The diffuse structure around the cell body and the stalk is a sheath whose synthesis is induced in response to environmental stress 39 . All data are representative of at least two biological repetitions analyzing at least 100 cells each.…”

Section: Figurementioning

confidence: 99%

“…PD denotes the permanent draft status of the genome. (g) DIC micrographs of the sequenced strains in (f) except for A. benevestitus , which was previously published 39 . Scale bars, 1 μm.…”

Section: Figurementioning

confidence: 99%

Sequential evolution of bacterial morphology by co-option of a developmental regulator

Jiang

Brown

Ducret

et al. 2014

Nature

119

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What mechanisms underlie the transitions responsible for the diverse shapes observed in the living world? While bacteria display a myriad of morphologies1, the mechanisms responsible for the evolution of bacterial cell shape are not understood. We investigated morphological diversity in a group of bacteria that synthesize an appendage-like extension of the cell envelope called the stalk2,3. The location and number of stalks varies among species, as exemplified by three distinct sub-cellular positions of stalks within a rod-shaped cell body: polar in the Caulobacter genus, and sub-polar or bi-lateral in the Asticcacaulis genus4. Here we show that a developmental regulator of Caulobacter crescentus, SpmX5, was co-opted in the Asticcacaulis genus to specify stalk synthesis at either the sub-polar or bi-lateral positions. We show that stepwise evolution of a specific region of SpmX led to the gain of a new function and localization of this protein, which drove the sequential transition in stalk positioning. Our results indicate that evolution of protein function, co-option, and modularity are key elements in the evolution of bacterial morphology. Therefore, similar evolutionary principles of morphological transitions apply to both single-celled prokaryotes and multicellular eukaryotes.

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Asticcacaulis benevestitus sp. nov., a psychrotolerant, dimorphic, prosthecate bacterium from tundra wetland soil

Cited by 39 publications

References 22 publications

Microbiota in foods from Inuit traditional hunting

Microbiota in foods from Inuit traditional hunting

Multisubstrate Isotope Labeling and Metagenomic Analysis of Active Soil Bacterial Communities

Sequential evolution of bacterial morphology by co-option of a developmental regulator

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