Astragalin Promotes Osteoblastic Differentiation in MC3T3-E1 Cells and Bone Formation in vivo

Liu, Li; Wang, Dan; Yao, Qing; Xu, Maolei; Zhou, Ling; Xu, Wei; Liu, Xiaona; Ye, Lei; Yue, Shi‐Jun; Zheng, Qiusheng; Li, Defang

doi:10.3389/fendo.2019.00228

Cited by 40 publications

(28 citation statements)

References 46 publications

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“…However, discussed results were inconsistent and contradictory, indicating that miR-21 may promote, as well as inhibit osteogenic differentiation, what can be correlated with diverse cellular plasticity of MSCs and their origin. To minimize this issue, we used the model of pre-osteoblast MC3T3 cell line that represents a stable and reproducible model for studies related with signaling pathways crucial for proliferation and differentiation of osteoblast [37][38][39][40][41]. Furthermore, under osteogenic conditions MC3T3 pre-osteoblasts synthesize and assemble collagenous extracellular matrix with organization and mineralization that resembles bone [37].…”

Section: Discussionmentioning

confidence: 99%

The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro

Śmieszek

Marcinkowska

Pielok

et al. 2020

Cells

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MiR-21 is being gradually more and more recognized as a molecule regulating bone tissue homeostasis. However, its function is not fully understood due to the dual role of miR-21 on bone-forming and bone-resorbing cells. In this study, we investigated the impact of miR-21 inhibition on pre-osteoblastic cells differentiation and paracrine signaling towards pre-osteoclasts using indirect co-culture model of mouse pre-osteoblast (MC3T3) and pre-osteoclast (4B12) cell lines. The inhibition of miR-21 in MC3T3 cells (MC3T3inh21) modulated expression of genes encoding osteogenic markers including collagen type I (Coll-1), osteocalcin (Ocl), osteopontin (Opn), and runt-related transcription factor 2 (Runx-2). Inhibition of miR-21 in osteogenic cultures of MC3T3 also inflected the synthesis of OPN protein which is essential for proper mineralization of extracellular matrix (ECM) and anchoring osteoclasts to the bones. Furthermore, it was shown that in osteoblasts miR-21 regulates expression of factors that are vital for survival of pre-osteoclast, such as receptor activator of nuclear factor κB ligand (RANKL). The pre-osteoclast cultured with MC3T3inh21 cells was characterized by lowered expression of several markers associated with osteoclasts’ differentiation, foremost tartrate-resistant acid phosphatase (Trap) but also receptor activator of nuclear factor-κB ligand (Rank), cathepsin K (Ctsk), carbonic anhydrase II (CaII), and matrix metalloproteinase (Mmp-9). Collectively, our data indicate that the inhibition of miR-21 in MC3T3 cells impairs the differentiation and ECM mineralization as well as influences paracrine signaling leading to decreased viability of pre-osteoclasts.

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Section: Discussionmentioning

confidence: 99%

The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro

Śmieszek

Marcinkowska

Pielok

et al. 2020

Cells

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“…The activity of ALP was evaluated by the p ‐nitrophenyl phosphate as substrate and measured at 405 nm (Ozeki, Aoki, & Fukui, 2008). Cells were treated as the previous report (Liu et al, 2019; Liu, Zhuang, Ouyang, & Yu, 2019) and the ALP assay kit was applied to measure the ALP activity according to the manufacturer's instruction. BCIP/NBT solution was applied for the ALP staining.…”

Section: Methodsmentioning

confidence: 99%

Cycloastragenol protects against glucocorticoid‐induced osteogenic differentiation inhibition by activating telomerase

Zeng

et al. 2020

Phytotherapy Research

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Glucocorticoid‐induced osteoporosis (GIOP) that is mainly featured as low bone density and increased risk of fracture is prone to occur with the administration of excessive glucocorticoids. Cycloastragenol (CAG) has been verified to be a small molecule that activates telomerase. Studied showed that up‐regulated telomerase was associated with promoting osteogeneic differentiation, so we explored whether CAG could promote osteogenic differentiation to protect against GIOP and telomerase would be the target that CAG exerted its function. Our results demonstrated that CAG prominently increased the ALP activity, mineralization, mRNA of runt‐related transcription factor 2, osteocalcin, osteopontin, collagen type I in both MC3T3‐E1 cells and dexamethasone (DEX)‐treated MC3T3‐E1 cells. CAG up‐regulated telomerase reverse transcriptase and the protective effect of CAG was blocked by telomerase inhibitor TMPyP4. Moreover, CAG improved bone mineralization in DEX‐induced bone damage in a zebrafish larvea model. Therefore, the study showed that CAG could alleviate the osteogenic differentiation inhibition induced by DEX in vitro and in vivo, and CAG might be considered as a candidate drug for the treatment of GIOP.

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“…29 Non-tea polyphenols, such as astragalin and sciadopitysin, have been shown to increase osteoblast differentiation and mineralization through their ability to function as antioxidants and decrease oxidant stress. 30,31 Interestingly, despite a normalized quantity of polyphenols from each tea, there were differences in mineralization between the tea types (GT/BT/GR/RR > WT, GT = BT = GR, RR > BT/GT) suggesting that the unique polyphenol profile of each tea may be responsible for the observed differences in mineralization between tea types. In addition, the mineralization data do not follow the antioxidant capacities of the teas.…”

Section: Discussionmentioning

confidence: 99%

Black and Green Tea as Well as Specialty Teas Increase Osteoblast Mineralization with Varying Effectiveness

McAlpine

Gittings

MacNeil

et al. 2021

Journal of Medicinal Food

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Many human studies suggest a benefit of tea consumption on bone health. The study objective was to compare the ability of different tea types to promote mineralization. Saos-2 cells underwent mineralization (5 days) in the presence of tea (white: WT, green: GT, black: BT, green rooibos: GR, or red rooibos: RR; 1 lg/mL of polyphenols) or control. Total polyphenol content (TPC, Folin-Ciocalteu's reagent), antioxidant capacity (2,2-diphenyl-1-picrylhydrazyl [DPPH] scavenging), mineralization (Alizarin Red staining), gene expression quantitative reverse transcription PCR (RT-qPCR), and cell activity (3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide assay) were determined. TPC was highest in GT and BT. The ability of each tea to inhibit DPPH also differed (WT, GT > RR) after normalizing for polyphenol quantity. Each tea increased mineralization and differences were observed among types (GT/BT/GR/RR > WT, GT = BT = GR, RR > BT/GT). mRNA expression of alkaline phosphatase (ALP) and ectonucleotide pyrophosphatase/phosphodiesterase (NPP1) remained unchanged, whereas osteopontin (OPN) and sclerostin (SOST) were reduced in cells treated with tea, regardless of type. At 24-and 48-h postexposure to tea, cell activity was greater in cells receiving any of the teas compared with vehicle control. Supplementation increased mineralization regardless of tea type with both rooibos teas and black tea stimulating greater mineralization than WT, whereas green tea is similar to the others. While future study is needed to confirm in vivo effects, the results suggest that consuming any of the teas studied may benefit bone health.

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Astragalin Promotes Osteoblastic Differentiation in MC3T3-E1 Cells and Bone Formation in vivo

Cited by 40 publications

References 46 publications

The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro

The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro

Cycloastragenol protects against glucocorticoid‐induced osteogenic differentiation inhibition by activating telomerase

Black and Green Tea as Well as Specialty Teas Increase Osteoblast Mineralization with Varying Effectiveness

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