Astragallus mollissimus plant extract: a strategy to reduce ruminal methanogenesis

Ochoa-García, Pedro Antonio; Anderson, Robin C.; Arévalos-Sánchez, Martha María; Rodríguez-Almeida, Felipe Alonso; Félix-Portillo, Monserrath; Muro-Reyes, Alberto; Božić, Aleksandar; Arzola-Alvarez, Claudio; Corral-Luna, Agustin

doi:10.1007/s11250-021-02882-1

Cited by 6 publications

(6 citation statements)

References 38 publications

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“…While ethyl nitroacetate exhibited quite modest antimicrobial activity against E. coli O157:H7 and S. Typhimurium, it was very potent at modulating methane production. Likewise, reductions in methane production observed in all the nitrocompound-treated incubations in the present screening study are consistent with observations from earlier studies conducted either with 100% carbon dioxide, as was performed in the present study [ 13 , 38 , 39 ], or with a 50:50 hydrogen:carbon dioxide as the initial gas phase [ 13 , 40 , 41 ]. The studies initiated with the 50:50 hydrogen:carbon dioxide gas phase were designed to ensure the provision of a non-limiting amount of reducing substrate.…”

Section: Discussionsupporting

confidence: 92%

Section: Discussioncontrasting

confidence: 65%

“…Evidence reported in the earlier work indicated that some nitrocompounds may act both as direct inhibitors against rumen methanogens and as alternative electron acceptors [13,39], however, based on the low recovery of reducing equivalents in the present study it is unlikely that appreciable amounts of ethyl nitroacetate were reduced here. The accumulation of hydrogen, or contrastingly its microbial consumption, in nitrocompoundtreated incubations may be influenced by the presence and activity of a hydrogen-oxidizing, nitro-compound-metabolizing bacterium, Denitrobacterium detoxificans [39,44]. This bacterium is normally present at less than 10 3 cells/mL in rumen populations having no prior exposure to the nitro compounds and although its numbers can be dramatically increased to >10 6 cells/mL in populations adapted to the nitro compounds, likely, this had not occurred in the present incubations [45].…”

Section: Discussioncontrasting

confidence: 65%

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Assessment of Potential Anti-Methanogenic and Antimicrobial Activity of Ethyl Nitroacetate, α-Lipoic Acid, Taurine and L-Cysteinesulfinic Acid In Vitro

Levent,

Božić,

Petrujkić

et al. 2023

Microorganisms

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Livestock producers need new technologies to maintain the optimal health and well-being of their animals while minimizing the risks of propagating and disseminating pathogenic and antimicrobial-resistant bacteria to humans or other animals. Where possible, these interventions should contribute to the efficiency and profitability of animal production to avoid passing costs on to consumers. In this study, we examined the potential of nitroethane, 3-nitro-1-propionate, ethyl nitroacetate, taurine and L-cysteinesulfinic acid to modulate rumen methane production, a digestive inefficiency that results in the loss of up to 12% of the host’s dietary energy intake and a major contributor of methane as a greenhouse gas to the atmosphere. The potential for these compounds to inhibit the foodborne pathogens, Escherichia coli O157:H7 and Salmonella Typhimurium DT104, was also tested. The results from the present study revealed that anaerobically grown O157:H7 and DT104 treated with the methanogenic inhibitor, ethyl nitroacetate, at concentrations of 3 and 9 mM had decreased (p < 0.05) mean specific growth rates of O157:H7 (by 22 to 36%) and of DT104 (by 16 to 26%) when compared to controls (0.823 and 0.886 h−1, respectively). The growth rates of O157:H7 and DT104 were decreased (p < 0.05) from controls by 31 to 73% and by 41 to 78% by α-lipoic acid, which we also found to inhibit in vitro rumen methanogenesis up to 66% (p < 0.05). Ethyl nitroacetate was mainly bacteriostatic, whereas 9 mM α-lipoic acid decreased (p < 0.05) maximal optical densities (measured at 600 nm) of O157:H7 and DT104 by 25 and 42% compared to controls (0.448 and 0.451, respectively). In the present study, the other oxidized nitro and organosulfur compounds were neither antimicrobial nor anti-methanogenic.

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Section: Discussionsupporting

confidence: 92%

Section: Discussioncontrasting

confidence: 65%

Section: Discussioncontrasting

confidence: 65%

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Assessment of Potential Anti-Methanogenic and Antimicrobial Activity of Ethyl Nitroacetate, α-Lipoic Acid, Taurine and L-Cysteinesulfinic Acid In Vitro

Levent,

Božić,

Petrujkić

et al. 2023

Microorganisms

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“…In addition, it was found that the addition of ruminal fermentation modifiers including tannins, saponins, halogenated chemical compounds, essential oils, and ionophore can reduce CH 4 emissions remarkedly [ 3 ]. However, these strategies not only increase the cost of animal husbandry but decrease the abundances of cellulolytic bacteria [ 7 , 8 ], lead to intoxication [ 9 ], and the efficacy of them varies across studies [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of 3-Nitropropionic Acid at Different Doses on In Vitro Rumen Fermentation, Digestibility, and Methane Emissions of Grazing Yak and Cattle

Guo,

Wang,

Zhang

et al. 2024

Animals

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3-nitropropionic acid (3NPA) has been proposed as an useful modifier to mitigate ruminal enteric methane emissions. However, few studies investigated the effects of 3NPA on ruminal fermentation characteristics of grazing ruminants in vitro. Rumen fluid from grazing yak and cattle were collected and incubated with additions of 0, 8, and 16 mM 3NPA. The total gas production, CH4 production, and dry matter digestibility significantly decreased with increasing 3NPA doses in both ruminant species (p < 0.05) and methane production decreased to almost 100% in cattle at 8 mM NPA but not yak, while H2 accumulation showed an opposite trend. The total fatty acid (TVFA) production, acetate concentration, and propionate concentration in cattle decreased as 3NPA doses increased at 12 and 24 h incubation. For yak, the H2 accumulation reached its apex at 8 mM NPA (p < 0.05). The TVFA in yak decreased significantly with increasing 3NPA doses at 12 and 72 h incubation. Moreover, the acetate concentration and propionate concentration in yak decreased as 3NPA doses increased at 12 and 24 h incubation. Overall, these findings demonstrated that 3NPA could be used as a strategy to mitigate methane emissions; although, it negatively affected the dry matter degradability in vitro.

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“…Denitrobacterium detoxificans, appears at present to be the only known ruminal bacteria to exhibit appreciable nitroalkane-metabolizing activity (15,16). Other xenobiotic short chain nitrocompounds such as nitroethane, 2-nitroethanol, 2-nitro-1-propanol, dimethyl-2-nitroglutarate, 2-nitro-methyl-propionate, and ethyl-2-nitroacetate have also been shown to inhibit ruminal methane production in vitro (17)(18)(19)(20)(21)(22). In the case of 3-nitro-1propionate, 3-nitro-1-propanol and nitroethane, their reduction to their respective amines β-alanine, 3-amino-1-propanol and ethylamine has been demonstrated (23,24).…”

Section: Introductionmentioning

confidence: 99%

Dynamics of Gastrointestinal Activity and Ruminal Absorption of the Methane-Inhibitor, Nitroethane, in Cattle

et al. 2022

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Nitroethane is a potent methane-inhibitor for ruminants but little is known regarding simultaneous effects of repeated administration on pre- and post-gastric methane-producing activity and potential absorption and systemic accumulation of nitroethane in ruminants. Intraruminal administration of 120 mg nitroethane/kg body weight per day to Holstein cows (n = 2) over a 4-day period transiently reduced (P < 0.05) methane-producing activity of rumen fluid as much as 3.6-fold while concomitantly increasing (P < 0.05) methane-producing activity of feces by as much as 8.8-fold when compared to pre-treatment measurements. These observations suggest a bacteriostatic effect of nitroethane on ruminal methanogen populations resulting in increased passage of viable methanogens to the lower bovine gut. Ruminal VFA concentrations were also transiently affected by nitroethane administration (P < 0.05) reflecting adaptive changes in the rumen microbial populations. Mean (± SD) nitroethane concentrations in plasma of feedlot steers (n = 6/treatment) administered 80 or 160 mg nitroethane/kg body weight per day over a 7-day period were 0.12 ± 0.1 and 0.41 ± 0.1 μmol/mL 8 h after the initial administration indicating rapid absorption of nitroethane, with concentrations peaking 1 day after initiation of the 80 or 160 mg nitroethane/kg body weight per day treatments (0.38 ± 0.1 and 1.14 ± 0.1 μmol/mL, respectively). Plasma nitroethane concentrations declined thereafter to 0.25 ± 0.1 and 0.78 ± 0.3 and to 0.18 ± 0.1 and 0.44 ± 0.3 μmol/mL on days 2 and 7 for the 80 or 160 mg nitroethane/kg body weight per day treatment groups, respectively, indicating decreased absorption due to increased ruminal nitroethane degradation or to more rapid excretion of the compound.

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Astragallus mollissimus plant extract: a strategy to reduce ruminal methanogenesis

Cited by 6 publications

References 38 publications

Assessment of Potential Anti-Methanogenic and Antimicrobial Activity of Ethyl Nitroacetate, α-Lipoic Acid, Taurine and L-Cysteinesulfinic Acid In Vitro

Assessment of Potential Anti-Methanogenic and Antimicrobial Activity of Ethyl Nitroacetate, α-Lipoic Acid, Taurine and L-Cysteinesulfinic Acid In Vitro

Effect of 3-Nitropropionic Acid at Different Doses on In Vitro Rumen Fermentation, Digestibility, and Methane Emissions of Grazing Yak and Cattle

Dynamics of Gastrointestinal Activity and Ruminal Absorption of the Methane-Inhibitor, Nitroethane, in Cattle

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