Astragaloside IV Attenuates the Myocardial Injury Caused by Adriamycin by Inhibiting Autophagy

Abstract

Astragaloside IV (ASIV) is the main active component of Astragalus, and can ameliorate cardiomyocyte hypertrophy, apoptosis and fibrosis. In this experiment, we studied how ASIV reduces the cardiotoxicity caused by adriamycin and protects the heart. To this end, rats were randomly divided into the control, ADR, ADR + ASIV and ASIV groups (n = 6). Echocardiography was used to observe cardiac function, HE staining was used to observe myocardial injury, TUNEL staining was used to observe myocardial cell apoptosis, and immunofluorescence and Western blotting was used to observe relevant proteins expression. Experiments have shown that adriamycin can damage heart function in rats, and increase the cell apoptosis index, autophagy level and oxidative stress level. Further results showed that ADR can inhibit the PI3K/Akt pathway. ASIV treatment can significantly improve the cardiac function of rats treated with ADR and regulate autophagy, oxidative stress and apoptosis. Our findings indicate that ASIV may reduce the heart damage caused by adriamycin by activating the PI3K/Akt pathway.

Astragaloside IV inhibits idiopathic pulmonary fibrosis through activation of autophagy by miR-21-mediated PTEN/PI3K/AKT/mTOR pathway

“…Such as, AS-IV improves renal fibrosis through modulating the SIRT1-NF-κB pathway along with autophagy activation [43]. AS-IV ameliorates cardiomyocyte apoptosis and fibrosis regulating autophagy [44]. In our study, we proved that ASV-IV induced autophagy in IPF through TEM and detection of autophagy-related genes, which was in line with previous study [45].…”

Astragaloside IV Ameliorates Isoprenaline-Induced Cardiac Fibrosis in Mice via Modulating Gut Microbiota and Fecal Metabolites

“…Astragaloside IV (AS-IV) is a main active ingredient extracted from Huangqi, a traditional edible and medicinal herb that has been used for over 2000 years ( Ren et al., 2013 ). Previous evidence suggested that Astragaloside IV exerts diverse biological activities, such as anti-oxidation ( Luo et al., 2021 ), suppressing inflammation ( Wan et al., 2018 ), modulating gut microbiota and gut-derived metabolites ( He Q. et al, 2020 ; Gong et al., 2021 ). AS-IV has also shown notable pharmacological effects on anti-myocardial fibrosis ( Lin et al., 2019 ; Wei et al., 2020 ).…”

Astragaloside IV attenuates sunitinib-associated cardiotoxicity by inhibiting COUP-TFII

“…In fact, we also observed SU-induced autophagy of H9c2 cell lines by using a transmission electron microscopy detection, which was suppressed by ASIV (data not shown). It has been reported that ASIV could attenuate the myocardial injury caused by adriamycin or lipopolysaccharide by inhibiting autophagy [ [37] , [38] , [39] ]. However, it remains elusive the role of autophagy in the antiapoptotic or cardioprotective effect of ASIV on SU-associated cardiotoxicity.…”