Astragaloside-IV prevents acute kidney injury and inflammation by normalizing muscular mitochondrial function associated with a nitric oxide protective mechanism in crush syndrome rats

Murata, Ikuo; Abe, Yuji; Yaginuma, Yuka; Yodo, Kayako; Kamakari, Yuka; Miyazaki, Yurika; Baba, Daichi; Shinoda, Yuko; Iwasaki, Takashi; Takahashi, Kyoko; Kobayashi, Jun; Inoue, Yutaka; Kanamoto, Ikuo

doi:10.1186/s13613-017-0313-2

Cited by 31 publications

(31 citation statements)

References 33 publications

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“…Additionally, icing treatment affects mitochondrial function after a long period of time. One of the characteristics of CS is that ROS production is enhanced in a relatively short time along with decreased mitochondrial function (6). In this study, ROS production peaked 6 h after the compression injury and was reduced by icing treatment.…”

Section: Discussionmentioning

confidence: 47%

“…One carotid artery was cannulated with a polyethylene catheter (PE-50 tubing) and was connected to a pressure transducer. Arterial blood samples from each group were obtained at 0.083, 0.5, 1, 3, 6, 12, and 24 h after reperfusion using the carotid artery catheter (6). The arterial levels of hematocrit (Hct), pH, base excess (BE), anion gap (AG), and lactate were analyzed using i-STAT300F blood gas analyzer and CG4+ and EC8+ cartridges (FUSO Pharmaceutical Industries, Osaka, Japan).…”

Section: Experimental Design Experimental Design-1 (Survival Rate)mentioning

confidence: 99%

“…The serum high mobility group box 1 (HMGB1), interleukin-6 (IL-6), and interleukin-10 (IL-10) were measured using HMGB1 ELISA Kit II (Shino-Test Co., Tokyo, Japan), Rat IL-6 Quantikine ® ELISA kit (R&D Systems, Inc., MN, USA), and Rat IL-10 Quantikine ® ELISA kit (R&D Systems, Inc., MN, USA) according to the manufacturer's instructions. ROS production in the injured gastrocnemius muscle was determined by measuring the concentration of thiobarbituric acid reactive substances (TBARS) (6). Myeloperoxidase (MPO) activity was determined by the method previously described by Murata et al (6).…”

Section: Determination Of Cytokines Reactive Oxygen Species (Ros)mentioning

confidence: 99%

“…ROS production in the injured gastrocnemius muscle was determined by measuring the concentration of thiobarbituric acid reactive substances (TBARS) (6). Myeloperoxidase (MPO) activity was determined by the method previously described by Murata et al (6). Isolation of mitochondria from the injured muscles for evaluation of mitochondrial function was performed using a mitochondrial isolation kit (Thermo Fisher Scientific K.K., Kanagawa, Japan).…”

Section: Determination Of Cytokines Reactive Oxygen Species (Ros)mentioning

confidence: 99%

“…We previously reported that in a CS model involving rats (5), they died due to cardiac failure following hyperkalemia; therefore, the outcomes in patients with CS might be improved by improving the condition of the muscles involved. Furthermore, improvements in the muscles are reported to be due to the anti-inflammatory and anti-oxidative effects that follow prevention of mitochondrial dysfunction (6).…”

Section: Introductionmentioning

confidence: 99%

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Icing treatment in rats with crush syndrome can improve survival through reduction of potassium concentration and mitochondrial function disorder effect

et al. 2019

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Crush syndrome (CS), a serious medical condition, which is characterized by damage to myocytes due to pressure and is associated with high mortality, even when patients receive fluid therapy. Icing therapy over the affected muscle has been reported to be effective in improving mitochondrial dysfunction and inflammation. These effects are thought to be secondary to improvements in the leakage of potassium and myoglobin from the damaged myocytes in the early stages of disease. However, their effects on the various symptoms of CS are unclear. It was hypothesized that treatment with icing will inhibit the influence of potassium by vasoconstriction, exert anti-inflammatory effects in the affected myocytes and improve mitochondrial function The CS model constructed by subjecting anesthetized rats to bilateral hindlimb compression with a rubber tourniquet for 5 h. The rats were then randomly divided into six groups: i) Sham; ii) CS without treatment (CS); iii) and iv) icing for 30 or 180 min over the entire hindlimb on CS rats (CI-30 and-180), respectively; and v) and vi) local icing for 30 or 180 min over the affected area on CS rats (CLI-30 and-180), respectively. Under continuous monitoring and recording of arterial blood pressures, blood and tissue samples were collected for biochemical analyses at designated time points prior to and following reperfusion. The survival rate, vital signs, and blood gas parameters in the CS group were lethal compared with the sham group. These were also improved in the CI-30 and CLI-30 groups compared with the CS group; however, they worsened in the CI-180 and CLI-180 groups due to hypothermia. The CI-30 and CLI-30 groups demonstrated tendencies of improvements compared with the CS group. Systemic inflammation and mitochondria dysfunction had improved in these groups compared with the CS group. We suggest icing therapy to temporarily prolong the viability after crush injury. Its effectiveness can be improved by combining it with other infusion therapies.

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Section: Discussionmentioning

confidence: 47%

Section: Experimental Design Experimental Design-1 (Survival Rate)mentioning

confidence: 99%

Section: Determination Of Cytokines Reactive Oxygen Species (Ros)mentioning

confidence: 99%

Section: Determination Of Cytokines Reactive Oxygen Species (Ros)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Icing treatment in rats with crush syndrome can improve survival through reduction of potassium concentration and mitochondrial function disorder effect

et al. 2019

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On‐Site Laser Photobiomodulation Treatment of Crushed Muscle Due to Prolonged Pressure in Rats

et al. 2021

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Background and Objectives: Crush injuries and prolonged pressure on muscles lead to bruises and sprains and, in most of the cases, cause distraction of the muscle and release of particles into the blood stream, causing renal and systemic complications in severe cases. Laser photobiomodulation treatment (i.e., laser phototherapy) is a method suggested to decrease the pressure damage in the first 24-48 hours after muscle injury, allowing a faster and more complete physical rehabilitation. We studied the efficacy of non-invasive laser photobiomodulation treatment as an on-site treatment for crush-injured gastrocnemius muscles, developing a moderate muscle crush injury model and aiming at decreasing damage extent while regaining physical competence faster. Study Design/Materials and Methods: Muscle crush injury was performed on 30 female Wistar rats using direct pressure for 10 minutes on the gastrocnemius muscle in both left and right hindlimbs. Immediately after the injury, only the left hindlimb were irradiated for 16 minutes (with 780 nm laser with a power of 250 mW, the energy at the target was 240 J, and the fluence was 1019 J/cm 2 ) for 1, 3, or 7 consecutive days, and sacrificed accordingly. During the follow-up period, 1, 3, or 7 days, both gastrocnemius muscles (of the treated and untreated hindlimbs) were evaluated for electrophysiology and functionality. Results: The laser photobiomodulation treatment showed a significant electrophysiological and functional recovery of the gastrocnemius muscle during the first 3 days after injury, in comparison with the untreated hindlimb. Conclusions: These preliminary results are promising, showing a significant effect of the laser photobiomodulation treatment during the first 3 days after the induction of the muscle crush injury, which is the most critical period in the clinical aspect. These findings suggest a therapeutic approach, which may help restore the muscle after crush injury.

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Protective role of Astragaloside IV in chronic glomerulonephritis by activating autophagy through PI3K/AKT/AS160 pathway

Chen

Liu

et al. 2020

Phytotherapy Research

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Astragaloside IV(AS-IV), a saponin purified from Astragalus membranaceus (Fisch.) Bge. var.mongholicus (Bge.) Hsiao, has been widely used in traditional Chinese medicine. However, the underlying mechanisms in treating chronic glomerular nephritis (CGN) have not been fully understood. The aim of the present study was to evaluate the potential mechanism of AS-IV on CGN. CGN rats were administrated with AS-IV at 10 mgÁkg −1 Ád −1 (ASL) and 20 mgÁkg −1 Ád −1 (ASH). Twenty four hour proteinuria, blood urea nitrogen (BUN), and serum creatinine (SCr) were detected. Hematoxylin-eosin (HE) and periodic acid-Schiff (PAS) staining were performed to evaluate the kidney lesion. Transmission electron microscope and GFP-RFP-LC3 transfection assay were used to monitor the effect of AS-IV on autophagy. IL-6 and IL-1β were detected. The expression of CyclinD1, PI3K/AKT/AS160 pathway and autophagy related proteins were detected by Western Blot. The results demonstrated that AS-IV improved kidney function, ameliorated kidney lesion, and diminished inflammatory in CGN rats. Further, both in vivo and vitro study demonstrated that AS-IV inhibited the proliferation of mesangial cells. AS-IV further displayed a remarkable effect on inhibiting the activation of PI3K/AKT/AS160 pathway and improved the activation of autophagy in vivo and vitro. These results suggested that AS-IV is a potential therapeutic agent for CGN and merits further investigation.

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Astragaloside-IV prevents acute kidney injury and inflammation by normalizing muscular mitochondrial function associated with a nitric oxide protective mechanism in crush syndrome rats

Cited by 31 publications

References 33 publications

Icing treatment in rats with crush syndrome can improve survival through reduction of potassium concentration and mitochondrial function disorder effect

Icing treatment in rats with crush syndrome can improve survival through reduction of potassium concentration and mitochondrial function disorder effect

On‐Site Laser Photobiomodulation Treatment of Crushed Muscle Due to Prolonged Pressure in Rats

Protective role of Astragaloside IV in chronic glomerulonephritis by activating autophagy through PI3K/AKT/AS160 pathway

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