Astragalus polysaccharides, chitosan and poly(I:C) obviously enhance inactivated Edwardsiella ictaluri vaccine potency in yellow catfish Pelteobagrus fulvidraco

Zhu, Wentao; Zhang, Yanqi; Zhang, Jiacheng; Yuan, Gailing; Liu, Xiaoling; Ai, Taoshan; Su, Jian

doi:10.1016/j.fsi.2019.01.033

Cited by 62 publications

(20 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the cellular level, IFNγ has been reported to facilitate ROS and NO production, to enhance defence against intracellular infections (Stafford et al, 1999). Consistent with the present study, work in teleosts demonstrated that poly (I:C) induced ifnγ to stimulate respiratory burst activity in vitro (Grayfer & Belosevic, 2009) and in vivo (Zhou et al, 2014;Zhu et al, 2019). are essential AAs for fish (Halver et al, 1957;National Research Council, 2011;Wilson, 2002) and are involved in energy metabolism (Roques et al, 2018b;Wagner et al, 2014).…”

Section: Fish Immune Gene Expressionsupporting

confidence: 86%

“…At the cellular level, IFN γ has been reported to facilitate ROS and NO production, to enhance defence against intracellular infections (Stafford et al ., ). Consistent with the present study, work in teleosts demonstrated that poly (I:C) induced ifnγ to stimulate respiratory burst activity in vitro (Grayfer & Belosevic, ) and in vivo (Zhou et al ., ; Zhu et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Poly (I:C) induced antiviral effects in vitro in O. tshawytscha-transfected embryonic cells CHSE-214 (Jensen et al, 2002b;Monjo et al, 2017) and in triploid O. tshawytscha spleen epithelia-derived cells CHSS (Semple et al, 2018). Mechanistically, poly (I:C) is a TLR3, SR-A and RLR ligand and binds to the receptors to stimulate IFN production and Mx proteins to elicit antiviral defence in salmonids and other teleosts (Arnemo et al, 2014;Das et al, 2009;Semple et al, 2018;Zhu et al, 2019). Similar results have been reported in salmonids following viral infections (Kim et al, 2009;Lockhart et al, 2004aLockhart et al, , 2007McBeath et al, 2007).…”

Section: Fish Immune Gene Expressionmentioning

confidence: 99%

See 2 more Smart Citations

Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge

Lulijwa

Mérien

Burdass

et al. 2020

Journal of Fish Biology

View full text Add to dashboard Cite

Polyinosinic:polycytidylic acid [poly (I:C)] was administered in vivo to Chinook salmon (Oncorhynchus tshawytscha) post‐smolts to determine the immune responses on haematological and cellular functional parameters, including spleen (SP), head kidney (HK) and red blood cell (RBC) cytokine expression, as well as serum metabolomics. Poly (I:C) in vivo (24 h exposure) did not affect fish haematological parameters, leucocyte phagocytic activity and phagocytic index, reactive oxygen species and nitric oxide production. Gas chromatography–mass spectrometry–based metabolomics revealed that poly (I:C) significantly altered the serum biochemistry profile of 25 metabolites. Metabolites involved in the branched‐chain amino acid/glutathione and transsulphuration pathways and phospholipid metabolism accumulated in poly (I:C)–treated fish, whereas those involved in the glycolytic and energy metabolism pathways were downregulated. At cytokine transcript level, poly (I:C) induced a significant upregulation of antiviral ifnγ in HK and Mx1 protein in HK, SP and RBCs. This study provides evidence for poly (I:C)–induced, immune‐related biomarkers at metabolic and molecular levels in farmed O. tshawytscha in vivo. These findings provide insights into short‐term effects of poly (I:C) at haematological, innate and adaptive immunity and metabolic levels, setting the stage for future studies.

show abstract

Section: Fish Immune Gene Expressionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 97%

Section: Fish Immune Gene Expressionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge

Lulijwa

Mérien

Burdass

et al. 2020

Journal of Fish Biology

View full text Add to dashboard Cite

show abstract

“…These results suggested that C3 is actively involved in antigen labeling, enhancing specific and nonspecific immune responses to eliminate antigens. In addition, superoxide dismutase and lysozyme are closely related to the immune levels in fish [37]. After the vaccination and challenge, TSOD and lysozyme showed an upward trend.…”

Section: Discussionmentioning

confidence: 95%

The Combination of Molecular Adjuvant CCL35.2 and DNA Vaccine Significantly Enhances the Immune Protection of Carassius auratus gibelio against CyHV-2 Infection

Huo

Fan

et al. 2020

Vaccines

Self Cite

View full text Add to dashboard Cite

Cyprinid herpesvirus 2 (CyHV-2) infection results in huge economic losses in gibel carp (Carassius auratus gibelio) industry. In this study, we first constructed recombinant plasmids pcORF25 and pcCCL35.2 as DNA vaccine and molecular adjuvant against CyHV-2, respectively, and confirmed that both recombinant plasmids could be effectively expressed in vitro and in vivo. Then, the vaccination and infection experiments (n = 50) were set as seven groups. The survival rate (70%) in ORF25/CCL35.2 group was highest. The highest specific antibody levels were found in ORF25/CCL35.2 group in major immune tissues by qRT-PCR, and confirmed in serum by ELISA assay, antibody neutralization titer, and serum incubation-infection experiments. Three crucial innate immune indices, namely C3 content, lysozyme, and total superoxide dismutase (TSOD) activities, were highest in ORF25/CCL35.2 group in serum. pcORF25/pcCCL35.2 can effectively up-regulate mRNA expressions of some important immune genes (IL-1β, IL-2, IFN-γ2, and viperin), and significantly suppress CyHV-2 replication in head kidney and spleen tissues. The minimal tissue lesions can be seen in ORF25/CCL35.2 group in gill, spleen, and trunk kidney tissues by histopathological examination. The results indicated that the combination of DNA vaccine pcORF25 and molecular adjuvant pcCCL35.2 is an effective method against CyHV-2 infection, suggesting a feasible strategy for the control of fish viral diseases.

show abstract

“…Vaccines, such as inactivated Edwardsiella ictaluri and infectious spleen and kidney necrosis virus, have been tested with chitosan in yellow catfish (Pelteobagrus fulvidraco) and Chinese perch (Siniperca chuasi), respectively. Chitosan enhanced incorporation into the host cells and improved fish survival rate and immune response, increasing IgM content, lysozyme activity and mRNA levels of interleukin (IL)-1β, IL-2 and interferon (IFN)-γ2 [133,134]. A mixture of COS and inactivated Vibrio anguillarum vaccine significantly reduced zebrafish mortality against Vibro anguillarum [135], while COS combined with inactivated Vibrio harveyi also markedly increased survival rate, IgM and the expression of immune-related genes, such as IL-1β, IL-16, tumor necrosis factor-alpha (TNF-α) and major histocompatibility complex class I alpha (MHC-Iα), in the grouper Epinephelus fuscoguttatus×Epinephelus lanceolatus [136].…”

Section: Chitosan Loading Inactivated Pathogensmentioning

confidence: 99%

Chitosan-Based Drug Delivery System: Applications in Fish Biotechnology

Rashidpour

Pablos

et al. 2020

Polymers

View full text Add to dashboard Cite

Chitosan is increasingly used for safe nucleic acid delivery in gene therapy studies, due to well-known properties such as bioadhesion, low toxicity, biodegradability and biocompatibility. Furthermore, chitosan derivatization can be easily performed to improve the solubility and stability of chitosan–nucleic acid polyplexes, and enhance efficient target cell drug delivery, cell uptake, intracellular endosomal escape, unpacking and nuclear import of expression plasmids. As in other fields, chitosan is a promising drug delivery vector with great potential for the fish farming industry. This review highlights state-of-the-art assays using chitosan-based methodologies for delivering nucleic acids into cells, and focuses attention on recent advances in chitosan-mediated gene delivery for fish biotechnology applications. The efficiency of chitosan for gene therapy studies in fish biotechnology is discussed in fields such as fish vaccination against bacterial and viral infection, control of gonadal development and gene overexpression and silencing for overcoming metabolic limitations, such as dependence on protein-rich diets and the low glucose tolerance of farmed fish. Finally, challenges and perspectives on the future developments of chitosan-based gene delivery in fish are also discussed.

show abstract

Astragalus polysaccharides, chitosan and poly(I:C) obviously enhance inactivated Edwardsiella ictaluri vaccine potency in yellow catfish Pelteobagrus fulvidraco

Cited by 62 publications

References 38 publications

Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge

Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge

The Combination of Molecular Adjuvant CCL35.2 and DNA Vaccine Significantly Enhances the Immune Protection of Carassius auratus gibelio against CyHV-2 Infection

Chitosan-Based Drug Delivery System: Applications in Fish Biotechnology

Contact Info

Product

Resources

About