Astrocytic Piezo1-mediated mechanotransduction determines adult neurogenesis and cognitive functions

Chi, Shaopeng; Cui, Yifu; Wang, Haiping; Jiang, Jinghui; Zhang, Tingxin; Sun, Shuang; Zhou, Zhuan; Zhong, Yi; Xiao, Bailong

doi:10.1016/j.neuron.2022.07.010

Cited by 73 publications

(63 citation statements)

References 66 publications

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“…Based on immunofluorescent staining with a specific antibody to Piezo1, we found Piezo1 distributed in a heterogeneous punctate pattern in primary ONH astrocytes which is also observed in previous findings in brain astrocytes (Chi et al, 2022) and epithelial cells (Gudipaty et al, 2017). We also observed that Piezo1 activation by its specific agonist Yoda1 could induce calcium influx in primary ONH astrocytes.…”

Section: Discussionsupporting

confidence: 90%

“…A recent study revealed that Piezo1 has more abundant expression in brain astrocytes than other mechano‐transducers, such as TRPV4 and TRPA1. The loss of Piezo1 can affect ATP‐dependent adult neurogenesis in vitro and in vivo (Chi et al, 2022). Besides, Piezo1 was highly upregulated in astrocytes surrounding amyloid plaques in an aging transgenic rat model of Alzheimer's disease and was closely associated with astrocyte reactive response (Velasco‐Estevez et al, 2018; Velasco‐Estevez et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes

Wan

Wang

Fan

et al. 2023

Glia

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Optic nerve head (ONH) astrocytes provide structural and metabolic support to neuronal axons in developmental, physiological, and pathological progression. Mechanosensitive properties of astrocytes allow them to sense and respond to mechanical cues from the local environment. We confirmed that ONH astrocytes express the mechanosensitive ion channel Piezo1 in vivo. By manipulating Piezo1 knockdown or overexpression in vitro, we found that Piezo1 is necessary but insufficient for ONH astrocyte proliferation. Loss of Piezo1 can lead to cell cycle arrest at G0/G1 phase, a possible mechanism involving decreased yes‐associated protein (YAP) nuclear localization and downregulation of YAP‐target cell cycle‐associated factors, including cyclin D1 and c‐Myc. Gene ontology enrichment analysis of differential expression genes from RNA‐seq data indicates that the absence of Piezo1 affects biological processes involving cell division. Our results demonstrate that Piezo1 is an essential regulator in cell cycle progression in ONH astrocytes.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes

Wan

Wang

Fan

et al. 2023

Glia

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“…It has been postulated that channel-mediated ATP release engages under pathological conditions ( 52, 53 ). However, accumulating evidence suggests that these mechanisms are also involved in basal ATP gliotransmission ( 54, 55, 13, 8, 15 ). While connexin hemichannels ( 13 ), pannexin 1 ( 56, 55 ), calcium homeostasis modulator 2 ( 8 ), volume-regulated anion channels ( 57 ), and maxi-anion channels ( 58 ) have been identified as ATP release channels in astrocytes, results in this study with CBX (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Not only is ATP released from astrocytes in response to neuronal inputs, but Koizumi et al found tetrodotoxin-insensitive ATP release in neuron-glia co-culture (12). Tonic ATP release was reported in brain slice preparations (13)(14)(15), but it was unclear whether astrocytes release ATP spontaneously or in response to the basal neuronal firing. Investigating basal ATP release activity will provide a further perspective on how astrocytes are involved in the neuron network function.…”

Section: Introductionmentioning

confidence: 99%

Spontaneous and multifaceted ATP release from astrocytes at the scale of hundreds of synapses

Hatashita

Fujita

et al. 2022

Preprint

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Astrocytes participate in information processing by releasing neuroactive substances termed gliotransmitters, including ATP. Individual astrocytes come into contact with thousands of synapses with their ramified structure, but the spatiotemporal dynamics of ATP gliotransmission remain unclear, especially in physiological brain tissue. Using a genetically encoded fluorescent sensor, GRABATP1.0, we discovered that extracellular ATP increased locally and transiently in absence of stimuli in neuron-glia co-cultures, cortical slices, and the anesthetized mouse brain. Spontaneous ATP release events were tetrodotoxin-insensitive but suppressed by gliotoxin, fluorocitrate, and typically spread over 50—250 µm2area at concentrations capable of activating purinergic receptors. Besides, most ATP events did not coincide with Ca2+transients. Clustering analysis revealed that these events followed multiple distinct kinetics, and blockade of exocytosis only decreased a minor group of slow events. Overall, astrocytes spontaneously release ATP through multiple mechanisms, mainly in non-vesicular and Ca2+-independent manners, thus potentially regulating hundreds of synapses all together.

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“…Engineering of biologically active surfaces in vascular reconstructions is associated with the study and creation of micro/nanostructured surface reliefs that mimic the characteristics of the extracellular matrix and realize their activity in relation to cellular elements through mechanochemical signaling systems [ 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. Morphological and dimensional characteristics of relief elements, as well as the degree of their ordering, can selectively influence the processes of differentiation, proliferation, and apoptosis [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of PHA Substrate Surface Characteristics on the Functional State of Endothelial Cells

Ryltseva

Dudaev

Menzyanova

et al. 2023

JFB

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The needs of modern regenerative medicine for biodegradable polymers are wide and varied. Restoration of the viability of the vascular tree is one of the most important components of the preservation of the usefulness of organs and tissues. The creation of vascular implants compatible with blood is an important task of vascular bioengineering. The function of the endothelial layer of the vessel, being largely responsible for the development of thrombotic complications, is of great importance for hemocompatibility. The development of surfaces with specific characteristics of biomaterials that are used in vascular technologies is one of the solutions for their correct endothelialization. Linear polyhydroxyalkanoates (PHAs) are biodegradable structural polymeric materials suitable for obtaining various types of implants and tissue engineering, having a wide range of structural and physicomechanical properties. The use of PHA of various monomeric compositions in endothelial cultivation makes it possible to evaluate the influence of material properties, especially surface characteristics, on the functional state of cells. It has been established that PHA samples with the inclusion of 3-hydroxyhexanoate have optimal characteristics for the formation of a human umbilical vein endothelial cell, HUVEC, monolayer in terms of cell morphology as well as the levels of expression of vinculin and VE-cadherin. The obtained results provide a rationale for the use of PHA copolymers as materials for direct contact with the endothelium in vascular implants.

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Astrocytic Piezo1-mediated mechanotransduction determines adult neurogenesis and cognitive functions

Cited by 73 publications

References 66 publications

Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes

Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes

Spontaneous and multifaceted ATP release from astrocytes at the scale of hundreds of synapses

Influence of PHA Substrate Surface Characteristics on the Functional State of Endothelial Cells

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