Asulam Resistance in Embryoids of Celery Tissue Cultures

Merrick, Marnin; Collin, H. A.

doi:10.1111/j.1469-8137.1982.tb03401.x

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…There is only a limited number of species where regenerated plants have been obtained and their seed progeny shown to be tolerant [8][9][10][11][12]. One approach that overcomes the problem of regeneration of selected cell lines is to carry out the in vitro screening with differentiating cultures [13]. Differentiation of embryos or shoots is initiated in the tissue culture in the presence of the selection treatments; which in this case would be NaC1.…”

Section: Introductionmentioning

confidence: 99%

Characterization of progeny of Coleus blumei following an in vitro selection for salt tolerance

Ibrahim

Collins

Collin

1992

Plant Cell Tiss Organ Cult

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An in vitro selection method was developed for Coleus blumei to enhance salt tolerance of this amenity species. Leaf disc explants were incubated on a Murashige & Skoog medium containing benzylaminopurine, 2 mg 1-1, and napthalene acetic acid, 1 mg 1-1, which initiated both callus and plantlets from the explants. A large number of explants were incubated on this differentiating medium containing 90 mM NaCI, which inhibited over 90% of plantlet formation. Surviving plantlets .were grown to maturity, when apical cuttings were taken and propagated. Plants were also allowed to flower and set seed. Cuttings from the selected regenerated plants showed consistently better growth in the presence of NaCI than unselected cuttings. Seed progeny of selected plants also showed more vigorous growth in the presence and absence of NaCI than progeny from unselected plants. The in vitro selection was compared with the results of an earlier in vivo selection to assess the contribution from tissue culture derived somaclonal variation. Progeny from the in vitro selection showed a higher level of tolerance than progeny from the in vivo selection.

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Section: Introductionmentioning

confidence: 99%

Characterization of progeny of Coleus blumei following an in vitro selection for salt tolerance

Ibrahim

Collins

Collin

1992

Plant Cell Tiss Organ Cult

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“…Norbury & Miflin. 1979) and tissue culturederived embryos (Merrick & Collin. 1982;Templeton-Sommers, 1981) have been found to be successful in screening for variation.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of asulam resistance in barley

1986

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Summary: Réesumé: Zusammenfassung Surface sterile excised embryos in barley var. Midas were used to establish whether the embryos could provide a screening procedure for increasing the levels of resistance to asulam in this variety. The embryos incubated in 1–5 mg 1−1 asulam were susceptible to the lowest concentration, but showed a linear response of increasing percentage mortality with increase in log dose of asulam in the same way as intact glasshouse grown seedlings sprayed with a range of 1–5 × 103 mg 1−1 asulam. There was a 100% mortality of the embryo‐derived seedlings in the 2–5 mg 1−1 asulam 25 days after the start of the incubation. During this time growth was recorded as percentage greening, leaf number and shoot length which all showed a marked decline with concentrations of asulam up to 5.0 mg 1−1. The inhibitory effect of asulam on growth was decreased by the addition of folic acid or 4‐aminobenzoic acid to the medium, suggesting that the mode of action of asulam as an inhibitor of folic acid synthesis was the same in the excised embryos as in intact plants. In order to screen for asulam resistance using the excised culture method, 1500 excised embryos were incubated on 5.0 mg 1−1 asulam. The resistance was measured by selecting those individuals which showed 75% or more growth compared with the untreated control. The selected individuals set self‐pollinated seed (S1), then excised embryos from the S1 seed were screened again for resistance to asulam and set seed again to produce further progeny (S2). A comparison of excised embryos from S1 and S2 seed with the parental stock showed enhanced resistance in both S1 and S2 where percentage greening and leaf number were used as the growth parameter, but no difference was recorded for shoot length between parent and selected progeny. Améelioration de la résistance de l'orge à l'asulame Des excisats embryonnaires stériles d'orge, variété Midas, ont servi àétudier si les embryons pouvaient fournir une méthode de sélection pour améliorer les niveaux de résistance à l'asulame chez cette variété. Les embryons placés en incubation dans des solutions de l à 5 mg 1−1 d'asulame étaient sensibles dès la concentration la plus basse, mais il y avait une réponse linéaire de l'augmentation de pourcentage de mortalité avec l'augmentation du logarithme de la dose d'asulame, de la même façon que sous serre avec des semis traités avec des doses de l à 5 g 1−1 d'asulame. Il y a eu une mortalité de 100% des semis issus des embryons 25 jours après le début de l'incubation avec 2 à 5 mg 1−1 d'asulame. Pendant ce temps, le développement était noté: % de surface verte, nombre de feuilles, longueur de tige diminuaient tous avec les concentrations d'asulame jusqu'à 5 mg 1−1. L'effet inhibiteur de l'asulam sur la croissance était réduit de moitié par l'adjonction d'acide folique ou d'acide 4 amino benzoïque, ce qui donne à penser que le mode d'action de l'asulame en tant qu'inhibiteur de la synthèse de l'acide folique est le même chez l'embryon excisé et chez la plante entière. En...

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