Asymbiotic Germination and Seedling Development of Terrestrial Orchid Bletilla striata Using in vitro and ex vitro Cultures

Kahraman, Mehmet; Cullum, F. J.

doi:10.16882/hortis.823961

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…Successful asymbiotic seed productions of several wild orchids have been reported, including Eulophia alta [10], Bletilla striata [11], Cyrtopodium paludicolum [12], (Spathoglottis plicata [13], Cremastra appendiculata (D. Don) Makino [14], and Paphiopedilum tigrinum [15]. Other essential elements influencing the success of in vitro asymbiotic seed cultivations and protocorm development include macro and micro element compositions, vitamins, sucrose, nitrogen, carbohydrates, and organic nutrients [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Developing Paraphalaenopsis labukensis (Shim, A. Lamb & C.L. Chan), an Orchid Endemic to Sabah, Borneo, Asymbiotic Seed Germination and In Vitro Seedling Development

et al. 2023

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Paraphalaenopsis labukensis Shim, A. Lamb & C.L. Chan (P. labukensis) is an endangered monopodial epiphytic orchid threatened due to habitat fragmentation and overharvesting. Consequently, this research aimed to achieve in vitro propagation of P. labukensis through asymbiotic seed germination and seedling development. This study focused on identification and optimizing capsule maturity, basal media and nutrient requirements. In addition, after hand pollinating the plant, their capsules’ flowers, length, girth, and colour variations were recorded weekly to ascertain morphological maturity characters of this species. Murashige and Skoog (MS), Knudson C (KC), and Vaccin and Went (VW) basal media were used to determine seed vegetation at 60, 90, and 120 days. Subsequently, for the proliferation and development of protocorms, the best basal media were those supplemented with different organic additives (coconut water (CW) and banana homogenate (BH)) and plant growth regulators (PGRs). As a result, 120 days after pollination (DAP) was chosen as the best capsule age for germination since the capsules had reached maturity. The highest germination rate was attained after 90 days of culture in Knudson C (KC) medium, with 98.78 ± 0.89%, followed by Murashige and Skoog (MS) medium, with 92.80 ± 3.26%. The KC supplemented with 0.5 mg/L NAA media documented a maximum percentage of 17.25 ± 0.96%. The 5 g/L (w/v) BH recorded a higher protocorm proliferation percentage than CW. Additionally, after 150 days of culture (DOC), this medium combination produced the most leaves, averaging four or five. The result of this present study has successfully established an effective in vitro propagation protocol for this species.

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Section: Introductionmentioning

confidence: 99%

Developing Paraphalaenopsis labukensis (Shim, A. Lamb & C.L. Chan), an Orchid Endemic to Sabah, Borneo, Asymbiotic Seed Germination and In Vitro Seedling Development

et al. 2023

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In Vitro Propagation of Cremastra appendiculata var. variabilis by Asymbiotic Seed Germination

Faisal

Seob²,

Kang³

et al. 2022

Horticulturae

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Cremastra appendiculata var. variabilis (Blume) I.D. Lund), also known as single-leaf cremastra (SLC), is a rare and threatened species native to Korea, and it has the potential to be grown as a beautiful flowering pot or garden plant. There is still no reliable strategy to multiply SLC. Thus, an effective method for propagating single-leaf cremastra was needed for its conservation and mass production. In the present study, we examined the effects of culture media, seed age, activated charcoal, and plant growth regulators on in vitro asymbiotic seed germination, secondary protocorm induction, and seedling formation. Asymbiotic seed germination of SLC was influenced by culture media, seed age, and their interaction. The addition of activated charcoal (500 mg/L) to the Murashige and Skoog (MS) medium increased the rate of germination. The seeds were best germinated (91.9%) by culturing on MS medium supplemented with activated charcoal (500 mg/L), α-naphthaleneacetic acid (3 µM), and kinetin (1 µM). The highest number (28.9) of secondary protocorms were produced when protocorms were cultured on MS medium containing 6-benzyladenine (4 µM) and kinetin (2 µM). When the protocorms were cultivated in a medium containing gibberellic acid (1 µM), they were able to transform into SLC with the highest success rate (78.7%). The propagation protocol described here may be helpful for SLC restoration programs and large-scale production.

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Ethnopharmacology of <i>Bletilla</i> orchid species: a comprehensive review on ethnobotany, phytochemistry and pharmacology

Fan,

Zhao,

Wang

et al. 2023

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Asymbiotic Germination and Seedling Development of Terrestrial Orchid Bletilla striata Using in vitro and ex vitro Cultures

Cited by 3 publications

References 30 publications

Developing Paraphalaenopsis labukensis (Shim, A. Lamb & C.L. Chan), an Orchid Endemic to Sabah, Borneo, Asymbiotic Seed Germination and In Vitro Seedling Development

Developing Paraphalaenopsis labukensis (Shim, A. Lamb & C.L. Chan), an Orchid Endemic to Sabah, Borneo, Asymbiotic Seed Germination and In Vitro Seedling Development

In Vitro Propagation of Cremastra appendiculata var. variabilis by Asymbiotic Seed Germination

Ethnopharmacology of <i>Bletilla</i> orchid species: a comprehensive review on ethnobotany, phytochemistry and pharmacology

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