Asymbiotic seed germination and in vitro seedling development of the threatened orchid Hoffmannseggella cinnabarina

Suzuki, Rogério Mamoru; Moreira, Vania Carolina; Pescador, Rosete; Ferreira, Wagner de Melo

doi:10.1007/s11627-012-9460-1

Cited by 29 publications

(23 citation statements)

References 17 publications

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“…Seed viability rates were evaluated using the tetrazolium test, following Pinheiro et al (). The method was previously evaluated (Alvarez‐Pardo et al ; Suzuki et al ) indicating that the percentage of seed viability, measured by tetrazolium test, was very similar to the respective percentage of seed germination for different orchid species. Samples of 200 seeds per fruit were analyzed with an optical microscope.…”

Section: Methodsmentioning

confidence: 93%

PHYLOGEOGRAPHIC STRUCTURE AND OUTBREEDING DEPRESSION REVEAL EARLY STAGES OF REPRODUCTIVE ISOLATION IN THE NEOTROPICAL ORCHIDEPIDENDRUM DENTICULATUM

et al. 2013

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Phylogeographic studies provide an important framework for investigating the mechanisms operating during the earliest stages of speciation, as reproductive barriers can be examined among divergent lineages in a geographic context. We investigated the evolution of early stages of intrinsic postmating isolation among different populations and lineages of Epidendrum denticulatum, a Neotropical orchid distributed across different biomes in South America. We estimated genetic diversity and structure for both nuclear and plastid markers, using a haplotype network, differentiation tests, Bayesian assignment analysis, and divergence time estimates of the main lineages. Reproductive barriers among divergent lineages were examined by analyzing seed viability following reciprocal crossing experiments. Strong plastid phylogeographic structure was found, indicating that E. denticulatum was restricted to multiple refuges during South American forest expansion events. In contrast, significant phylogeographic structure was not found for nuclear markers, suggesting higher gene flow by pollen than by seeds. Large asymmetries in seed set were observed among different plastid genetic groups, suggesting the presence of polymorphic genic incompatibilities associated with cytonuclear interactions. Our results confirm the importance of phylogeographic studies associated with reproductive isolation experiments and suggest an important role for outbreeding depression during the early stages of lineage diversification.

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Section: Methodsmentioning

confidence: 93%

PHYLOGEOGRAPHIC STRUCTURE AND OUTBREEDING DEPRESSION REVEAL EARLY STAGES OF REPRODUCTIVE ISOLATION IN THE NEOTROPICAL ORCHIDEPIDENDRUM DENTICULATUM

et al. 2013

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“…The percentage of viable seeds was determined by dividing the number of viable embryos by the total number of embryos analyzed. Previous studies showed that the percentage of seed viability estimated by the tetrazolium test was very similar to the percentage of seed germination in several species of orchids [77,78]. The effects of treatments on fruit set and seed viability were tested with a GLM with pollination treatment and populations as fixed factors and individuals as a random-effect factor.…”

Section: Methodsmentioning

confidence: 99%

Multiple hybridization events, polyploidy and low postmating isolation entangle the evolution of neotropical species of Epidendrum (Orchidaceae)

et al. 2014

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BackgroundHybridization and polyploidy are central processes in evolution and speciation. These mechanisms often lead to complex patterns of genetic variation and the creation of novel genotypes, which may establish if they become isolated from gene flow. However, in the absence of reproductive isolation, species boundaries might easily be disrupted. Here, we used a combination of AFLPs, chloroplast DNA markers and flow cytometry to investigate the evolutionary outcomes of hybridization between two endemic Ecuadorian species of Epidendrum (E. madsenii and E. rhopalostele) in three hybrid zones. Postmating isolation was also quantified to determine the role of this barrier in restraining gene flow between hybrids and the parental species. In addition, future ecological niche models were constructed to predict the outcomes of hybridization between these species.ResultsOur results confirmed the presence of hybrids in all hybrid zones, but revealed that a third parental species (E. falcisepalum) has contributed to one of the hybrid zones studied. Backcross genotypes were frequent in all hybrid zones, which was in accordance with the absence of strong reproductive barriers. The process of hybridization was highly asymmetric and followed in some cases by polyploidy. The projection of future niche models predicted a severe reduction in the area suitable for the occurrence of these species, although favorable conditions will still occur for the existence of the current hybrid zones.ConclusionsThe recurrent process of hybridization has compromised the genetic integrity of the parental species. Most individuals of the parental species can no longer be considered as pure-bred individuals because most were classified as backcrossed hybrids. Novel genetic lineages occur in all hybrid zones implying that hybrids are fertile and can compete with the parental species. These results, together with the prediction of suitable conditions for the future occurrence of these hybrid zones, highlight the importance of conserving these geographic areas as sources of novel taxonomic entities.

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“…Storage of the orchid seeds gives us the opportunity to conserve orchids; however, there are very few studies on the longevity of orchid seeds (Suzuki et al, 2012). Many species produce enormous numbers of tiny seeds, which would allow the storage of large numbers in a reasonable small space.…”

Section: Introductionmentioning

confidence: 99%

Asymbiotic seed germination and in vitro propagation of Brasiliorchis picta

Santos¹,

Smidt²,

Padial³

et al. 2016

Afr. J. Biotechnol.

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Seed storage method for in vitro germination and propagation from leaves of Brasiliorchis picta was developed. Seeds were harvested and stored at-20 and-80°C for 1, 3, 6, and 12 months and were germinated on Knudson C (KC), Murashige and Skoog (MS), half-strength MS (½ MS macro-and micronutrients), and woody plant medium (WPM). Seeds stored at-20°C, the recommended temperature for seed banks, had a high germination rate (76.0%) when cultivated in WPM after 12 months of storage. WPM is the best medium for seed germination and seedling development for both harvested and stored seeds, regardless of storage time and storage temperature. Whole leaf and leaf transversal thin cell layers (tTCL) from 3-month-old in vitro grown protocorms were cultured in ½ MS supplemented with 6benzyladenine (BA; 2.5, 5.0 and 10.0 µM) and thidiazuron (TDZ; 3.0, 6.0 and 9.0 µM) for 12 weeks. The highest frequency of regenerated protocorm-like bodies (PLBs) from explants (70.0%) occurred when whole leaves were cultured in medium containing 5.0 µM BA, whereas the best response for leaf TCL was with the basal section in medium containing 9 µM TDZ, in which PLBs developed in all regions of leaves. Plantlets were successfully acclimatized (with a survival rate of 97%) when vermiculite was used as a substrate.

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Asymbiotic seed germination and in vitro seedling development of the threatened orchid Hoffmannseggella cinnabarina

Cited by 29 publications

References 17 publications

PHYLOGEOGRAPHIC STRUCTURE AND OUTBREEDING DEPRESSION REVEAL EARLY STAGES OF REPRODUCTIVE ISOLATION IN THE NEOTROPICAL ORCHIDEPIDENDRUM DENTICULATUM

PHYLOGEOGRAPHIC STRUCTURE AND OUTBREEDING DEPRESSION REVEAL EARLY STAGES OF REPRODUCTIVE ISOLATION IN THE NEOTROPICAL ORCHIDEPIDENDRUM DENTICULATUM

Multiple hybridization events, polyploidy and low postmating isolation entangle the evolution of neotropical species of Epidendrum (Orchidaceae)

Asymbiotic seed germination and in vitro propagation of Brasiliorchis picta

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