“…Amphioxus oocyte dynamics including the nuage and the localization of maternal factors are still poorly characterized, but some maternal mRNAs in eggs have been reported. Some germ line marker mRNAs such as vasa, nanos, piwi1, pl10, bruno2, and tdrd (tudor-domain containing) are localized near the vegetal pole, and others such as pumilio, mago-nashi, and mex3 are near the animal poles with some ubiquitously distributed mRNAs (Wu et al, 2011;Zhang et al, 2013;Yue et al, 2015;Dailey et al, 2016). Seemingly corresponding to these bipolar distributions of the germ line markers, mRNAs related to Wnt/b-Catenin signaling are not localized to the vegetal side, from which gastrulation starts (Qian et al, 2013;Wang et al, 2016).…”