Asymmetric expression of a novel homeobox gene in vertebrate sensory organs

Deitcher, David L.; Fekete, DM; Cepko, Connie

doi:10.1523/jneurosci.14-02-00486.1994

Cited by 75 publications

(48 citation statements)

References 45 publications

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“…Thus, to assess whether presumptive otic ectoderm, isolated at 5ss, is committed to a definitive inner ear fate, we tested the ability of this tissue to express the otic marker Soho1 and to develop inner ear hair cells, the mechanosensory receptor of the inner ear (Fig. 1A) (Bartolami et al, 1991;Deitcher et al, 1994). Few (18%) presumptive otic explants isolated at 5ss and cultured for 24 hours expressed Soho1 (Fig.…”

Section: Otic Ectoderm Passes Through Multiple States Of Commitmentmentioning

confidence: 99%

Progressive restriction of otic fate: the role of FGF and Wnt in resolving inner ear potential

Freter

Muta²,

Mak³

et al. 2008

Development

146

221

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The development of the vertebrate inner ear is an emergent process. Its progression from a relatively simple disk of thickened epithelium within head ectoderm into a complex organ capable of sensing sound and balance is controlled by sequential molecular and cellular interactions. Fibroblast growth factor (FGF) and Wnt signals emanating from mesoderm and neural ectoderm have been shown to direct inner ear fate. However, the role of these multiple signals during inner ear induction is unclear. We demonstrate that the action of the FGFs and Wnts is sequential, and that their roles support a model of hierarchical fate decisions that progressively restrict the developmental potential of the ectoderm until otic commitment. We show that signalling by Fgf3 and Fgf19 is required to initiate a proliferative progenitor region that is a precursor to both the inner ear and the neurogenic epibranchial placodes. Significantly, we find that only after FGF action is attenuated can the subsequent action of Wnt signalling allow otic differentiation to proceed. In addition, gain and loss of function of Wnt-signalling components show a role for this signalling in repressing epibranchial fate. This interplay of signalling factors ensures the correct and ordered differentiation of both inner ear and epibranchial systems.

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Section: Otic Ectoderm Passes Through Multiple States Of Commitmentmentioning

confidence: 99%

Progressive restriction of otic fate: the role of FGF and Wnt in resolving inner ear potential

Freter

Muta²,

Mak³

et al. 2008

Development

146

221

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“…Rod distribution after retroviral misexpression of early patterning genes Ectopic expression of FoxG1, SOHo1, and GH6 The winged-helix transcription factor FoxG1 and the related homeodomain proteins, SOHo1 and GH6, are expressed in the anterior eye anlage and contribute to retinotectal map formation (Deitcher et al, 1994;Hatini et al, 1994;Stadler and Solursh, 1994;Yuasa et al, 1996;Schulte and Cepko, 2000). Expression of FoxG1 precedes that of SOHo1 and GH6.…”

Section: Distribution Of Rod Photoreceptors After Removal Of the Dorsmentioning

confidence: 99%

“…The transcription factors FoxG1, SOHo1, and GH6 are expressed in the anterior eye anlage (Deitcher et al, 1994;Hatini et al, 1994;Stadler and Solursh, 1994). When ectopically expressed, these molecules directly or indirectly repress EphA3 expression and perturb the proper mapping of some RGCs to the optic tectum (Yuasa et al, 1996;Schulte and Cepko, 2000;Takahashi et al, 2003).…”

Section: Molecular Mechanisms Specifying Rod Photoreceptor Distributionmentioning

confidence: 99%

“…The anterior expression of the transcription factor FoxG1 (BF-1) and the posterior expression of FoxD1 (BF-2) were observed quite early in the optic vesicle. This was followed slightly later by expression of the homeodomain protein mHmx1/cGH6 and the related protein SOHo-1 in the anterior optic cup of chick and medaka (Deitcher et al, 1994;Hatini et al, 1994;Stadler and Solursh, 1994;Adamska et al, 2001). Expression of the homeodomain protein cVax/mVax2 is observed in the ventral optic vesicle, whereas members of the T-box family of transcription factors can be observed in the dorsal optic vesicle (Gibson-Brown et al, 1998;Barbieri et al, 1999;Ohsaki et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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The Rod Photoreceptor Pattern Is Set at the Optic Vesicle Stage and Requires Spatially RestrictedcVaxExpression

Schulte¹,

Peters²,

Sen³

et al. 2005

J. Neurosci.

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How and when positional identities in the neural retina are established have been addressed primarily with respect to the topographic projections of retinal ganglion cells onto their targets in the brain. Although retinotectal map formation is a prominent manifestation of retinal patterning, it is not the only one. Photoreceptor subtypes are arranged in distinct, species-specific patterns. The mechanisms used to establish photoreceptor patterns have been relatively unexplored at the mechanistic level. We performed ablations of the eye anlage in chickens and found that removal of the anterior or dorsal optic vesicle caused loss of the area centralis, which is a rod-free central area of the retina, and severely disorganized other aspects of the rod pattern. These observations indicate that the anteroposterior and dorsoventral distribution of rods is determined by the optic vesicle stage. To investigate the molecular mechanisms involved, the rod distribution was analyzed after viral misexpression of several patterning genes that were previously shown to be important in positional specification of retinal ganglion cells. Ectopic expression of FoxG1, SOHo1, or GH6 transcription factors expressed in the anterior optic vesicle and/or optic cup, respectively, did not affect the rod pattern. This pattern therefore appears to be specified by an activity acting before, or in parallel with, these factors. In contrast, misexpression of the ventrally restricted transcription factor, cVax, severely disturbed the rod pattern.

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“…One of the first genes expressed in the otic placode proper is Soho1 (Deitcher et al, 1994). Its expression is first detected at around 9/10ss and is concomitant with the ability of an isolated otic placode to differentiate hair cells (Freter et al, 2008).…”

Section: Pax2 Influences Early Inner Ear Developmentmentioning

confidence: 99%

Pax2 modulates proliferation during specification of the otic and epibranchial placodes

Freter¹,

Muta²,

O’Neill³

et al. 2012

Developmental Dynamics

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Background: The inner ear and epibranchial ganglia of vertebrates arise from a shared progenitor domain that is induced by FGF signalling, the posterior placodal area (PPA), before being segregated by Wnt signalling. One of the first genes activated in the PPA is the transcription factor Pax2. Loss-of-and gain-of function studies have defined a role for Pax2 in placodal morphogenesis and later inner ear development, but have not addressed the role Pax2 plays during the formation and maintenance of the PPA. Results: To understand the role of Pax2 during the development of the PPA, we used over-expression and repression of Pax2. Both gave rise to a smaller otocyst and repressed the formation of epibranchial placodes. In addition, cell cycle analysis revealed that Pax2 suppression reduced proliferation of the PPA. Key FindingsNeither suppression nor over-expression of Pax2 affects the extent of the precursor region of the inner ear and epibranchial placodes, the PPA. Suppressing and over-expressing Pax2 does affect the differentiation of the inner ear and the epibranchial placodes. Pax2 suppression reduces proliferation of PPA precursors.

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Asymmetric expression of a novel homeobox gene in vertebrate sensory organs

Cited by 75 publications

References 45 publications

Progressive restriction of otic fate: the role of FGF and Wnt in resolving inner ear potential

Progressive restriction of otic fate: the role of FGF and Wnt in resolving inner ear potential

The Rod Photoreceptor Pattern Is Set at the Optic Vesicle Stage and Requires Spatially RestrictedcVaxExpression

Pax2 modulates proliferation during specification of the otic and epibranchial placodes

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