Toxoplasma gondii is the organism that is responsible for toxoplasmosis disease. Toxoplasma gondii: is a crucial obligate intracellular parasite of humans and animals worldwide and infects nearly one-third of humanity; the disease can be severe and can lead to abortion or neonate’s death. In addition, an immunocompromised individual may develop several syndromes such as encephalitis, chorioretinitis, congenital infection and neonatal mortality. In this study, our objective is to determine the prevalence of Toxoplasma gondii antibodies through blood transfusion. Therefore, our study was conducted from 1 May 2010 to 31 of May 2010 among Libyan donors. We tested 164 blood donors from different ages for Toxoplasma gondii antibodies in Tripoli, a capital city of Libya. The results showed that 33.5% of blood donors had positive IgG antibodies. Therefore, this study suggest blood screening with high-performance techniques for Toxoplasma gondii before blood transfusion should be routinely done to avoid severe complications.