“…The performance of these biocatalysts is promising because of their high regio-, stereo-, and chemoselectivity, an expanding enzyme portfolio, substrate scope and type of chemical reactions they can catalyse. [6][7][8] Currently, more than 60 OYEs are characterised from plantae, fungi, bacteria, algae and cyanobacteria, divided across different classes. 4,7 Class III represents thermophilic-like OYEs, among which the thermostable TsOYE (formerly CrS), isolated from Thermus scotoductus SA-01, 9 catalyses a wide range of activated alkene substrates and has potential for scale-up.…”