Asymmetric Involution of the Myocardial Field Drives Heart Tube Formation in Zebrafish

Rohr, Stefan; Otten, Cécile; Abdelilah‐Seyfried, Salim

doi:10.1161/circresaha.107.165241

Cited by 100 publications

(112 citation statements)

References 37 publications

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“…As the zebrafish is an anamniote, folding of the coelom and the heart fields does not occur. Instead, the heart fields merge to form a cardiac cone that becomes a tube by a process of asymmetric involution of the right side of the cone (Rohr et al, 2008;Stainier et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Zebrafish cardiac development requires a conserved secondary heart field

et al. 2011

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SUMMARYThe secondary heart field is a conserved developmental domain in avian and mammalian embryos that contributes myocardium and smooth muscle to the definitive cardiac arterial pole. This field is part of the overall heart field and its myocardial component has been fate mapped from the epiblast to the heart in both mammals and birds. In this study we show that the population that gives rise to the arterial pole of the zebrafish can be traced from the epiblast, is a discrete part of the mesodermal heart field, and contributes myocardium after initial heart tube formation, giving rise to both smooth muscle and myocardium. We also show that Isl1, a transcription factor associated with undifferentiated cells in the secondary heart field in other species, is active in this field. Furthermore, Bmp signaling promotes myocardial differentiation from the arterial pole progenitor population, whereas inhibiting Smad1/5/8 phosphorylation leads to reduced myocardial differentiation with subsequent increased smooth muscle differentiation. Molecular pathways required for secondary heart field development are conserved in teleosts, as we demonstrate that the transcription factor Tbx1 and the Sonic hedgehog pathway are necessary for normal development of the zebrafish arterial pole.

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Section: Discussionmentioning

confidence: 99%

Zebrafish cardiac development requires a conserved secondary heart field

et al. 2011

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“…Because both spaw and lft1 are required for establishment of laterality, any disturbance in their expression pattern is correlated with defects in organ placement in zebrafish and other organisms (33)(34)(35)(36). We assayed for effects on heart and gut laterality in ier2 and fibp1 morphants, using cmlc2 (37) and foxa3 (38) as markers to visualize the early development of these organs. Ier2 and Fibp1 morphant embryos have extensive heart laterality and looping deficits, showing a predominance of situs inversus (Fig.…”

Section: B D E-l)mentioning

confidence: 99%

FGF-dependent left–right asymmetry patterning in zebrafish is mediated by Ier2 and Fibp1

Hong

Dawid

2009

Proc. Natl. Acad. Sci. U.S.A.

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Establishment of left-right asymmetry in vertebrates requires nodal, Wnt-PCP and FGF signaling and involves ciliogenesis in a laterality organ. Effector genes through which FGF signaling affects laterality have not been described. We isolated the zebrafish ier2 and fibp1 genes as FGF target genes and show that their protein products interact. Knock down of these factors interferes with establishment of organ laterality and causes defective cilia formation in Kupffer's Vesicle, the zebrafish laterality organ. Cilia are also lost after suppression of FGF8, but can be rescued by injection of ier2 and fibp1 mRNA. We conclude that Ier2 and Fibp1 mediate FGF signaling in ciliogenesis in Kupffer's Vesicle and in the establishment of laterality in the zebrafish embryo.ciliogenesis ͉ Kupffer's vesicle ͉ laterality

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“…In addition, nok and has/prkci regulate myocardial cell polarity, adhesion and cell-shape changes, which contribute to heart morphogenesis (Yelon et al, 1999;HorneBadovinac et al, 2001;Peterson et al, 2001;Trinh et al, 2005;Rohr et al, 2006;Rohr et al, 2008). Phenotypic similarities between these mutants support the genetic and biochemical evidence from other systems for a direct interaction between the Crb-Nok and Par6-aPKC protein complexes.…”

Section: Introductionmentioning

confidence: 96%

Divergent polarization mechanisms during vertebrate epithelial development mediated by the Crumbs complex protein Nagie oko

Bit‐Avragim

Hellwig

Rudolph

et al. 2008

Journal of Cell Science

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The zebrafish MAGUK protein Nagie oko is a member of the evolutionarily conserved Crumbs protein complex and functions as a scaffolding protein involved in the stabilization of multi-protein assemblies at the tight junction. During zebrafish embryogenesis, mutations in nagie oko cause defects in both epithelial polarity and cardiac morphogenesis. We used deletion constructs of Nagie oko in functional rescue experiments to define domains essential for cell polarity, maintenance of epithelial integrity and cardiac morphogenesis. Inability of Nagie oko to interact with Crumbs proteins upon deletion of the PDZ domain recreates all aspects of the nagie oko mutant phenotype. Consistent with this observation, apical localization of Nagie oko within the myocardium and neural tube is dependent on Oko meduzy/Crumbs2a. Disruption of direct interactions with Patj or Lin-7, two other members of the Crumbs protein complex, via the bipartite L27 domains produces only partial nagie oko mutant phenotypes and does not impair correct junctional localization of the truncated Nagie oko deletion protein within myocardial cells. Similarly, loss of the evolutionarily conserved region 1 domain, which mediates binding to Par6, causes only a subset of the nagie oko mutant epithelial phenotypes. Finally, deletion of the C-terminus, including the entire guanylate kinase and the SH3 domains, renders the truncated Nagie oko protein inactive and recreates all features of the nagie oko mutant phenotype when tested in functional complementation assays. Our observations reveal a previously unknown diversity of alternative multi-protein assembly compositions of the Crumbs–Nagie-oko and Par6-aPKC protein complexes that are highly dependent on the developmental context.

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Asymmetric Involution of the Myocardial Field Drives Heart Tube Formation in Zebrafish

Cited by 100 publications

References 37 publications

Zebrafish cardiac development requires a conserved secondary heart field

Zebrafish cardiac development requires a conserved secondary heart field

FGF-dependent left–right asymmetry patterning in zebrafish is mediated by Ier2 and Fibp1

Divergent polarization mechanisms during vertebrate epithelial development mediated by the Crumbs complex protein Nagie oko

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