Asymmetric protoplast fusions between wild species and breeding lines of potato – effect of recipients and genome stability

Oberwalder, B.; Schilde-Rentschler, L.; Ruoss, B.; Wittemann, Silke; Ninnemann, Helga

doi:10.1007/s001220051028

Cited by 24 publications

(10 citation statements)

References 21 publications

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“…To check the minor differences in amplified products, it can be optimized with PCR reaction to obtain reproducible and interpretable results (CaetanoAnolles et al 1992). The use of RAPD for routine screening of somatic hybrids could be insufficient if the amount of DNA introgressed from one of the fusion partner is too low (i.e., below 8%; Oberwalder et al 1998). We confirmed the hybridity by standardization of PCR cycles which resulted in distinct 'species-specific diagnostic bands'.…”

Section: Discussionsupporting

confidence: 62%

Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl.

Tiwari

Poonam

Sarkar

et al. 2010

Plant Cell Tiss Organ Cult

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Interspecific potato somatic hybrids between Solanum tuberosum L. (di)haploid C-13 and 1 endosperm balance number non-tuberous wild species S. etuberosum Lindl. were produced by protoplasts electrofusion. The objective was to transfer virus resistance from this wild species into the cultivated potatoes. Post-fusion products were cultured in VKM medium followed by regeneration of calli in MS 13 K medium at 20°C under a 16-h photoperiod, and regenerants were multiplied on MS medium. Twenty-one somatic hybrids were confirmed by RAPD, SSR and cytoplasm (chloroplast/mitochondria) type analysis possessing species-specific diagnostic bands of corresponding parents. Tetraploid nature of these somatic hybrids was determined through flow cytometry analysis. Somatic hybrids showed intermediate phenotypes (plant, leaves and floral morphology) to their parents in glasshouse grown plants. All the somatic hybrids were malefertile. ELISA assay of somatic hybrids after artificial inoculation of Potato virus Y (PVY) infection reveals high PVY resistance.

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Section: Discussionsupporting

confidence: 62%

Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl.

Tiwari

Poonam

Sarkar

et al. 2010

Plant Cell Tiss Organ Cult

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“…to introduce important traits (Yamashita et al, 2002;Gavrilenko et al, 2003;Wang et al, 2003). It has been also well documented that chromosome elimination in the hybrid depends on other factors such as donor species, genetic distance, culture condition, ploidy level of fusion partners (Oberwalder et al, 1998). On the other hand, chromosome elimination decreases the negative impact from undesirable traits that are transferred to the somatic hybrids accompanying the desirable ones in standard symmetric fusions.…”

Section: Resultsmentioning

confidence: 95%

Interspecific hybrids between Medicago sativa L. and annual Medicago containing Alfafa weevil resistance

Mizukami

Kato²,

Takamizo

et al. 2005

Plant Cell Tiss Organ Cult

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Non-embryogenic protoplasts of Medicago rugosa and M. scutellata were electro-fused with iodoacetic acid-treated protoplasts of M. sativa (alfalfa). Putative somatic hybrid callus were obtained and some plants regenerated from both combinations. Hybridity of regenerants was confirmed by morphology, molecular means and cytological observations. Parental specific bands were recognized in somatic hybrids by Southern analysis. The somatic hybrids were perennial and their morphology was similar to M. sativa.Cytological observations were carried out on the somatic hybrids, their vegetative clones and self-pollinated offspring. Original somatic hybrids were aneuploids (2n=31-59), but during vegetative proliferation, their chromosome numbers reduced to 32. Those clones of hybrids formed seeds from M. sativa (+) M. rugosa by self-crossing. Chromosomal rearrangements within the parental genomes were observed in vegetative clones of hybrids and their S 1 offspring by Genomic in situ Hybridization (GISH). Some of S 1 offspring from M. sativa (+) M. rugosa showed better spring growth than parental M. sativa and tend to be tolerant to Alfalfa weevil. It was considered that these traits were introduced from the genome transferring M. rugosa chromosome to M. sativa. The cell fusion may still have a potential in transferring alien chromosomes in order to increase the genetic variation for crop breeding.Abbreviations: CTAB -cetyl trimethyl ammonium bromide; DAPI -4,6-diamino-2-phenylindole dihydrochloride; 2,4-D -2,4-dichlorophenoxi-acetic acid; GISH -Genomic in situ Hybridization; IOA -iodoacetamide

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“…The chromosome number of the set of regenerated plants from clone I-3 lay mostly in the range 13-17, which was somewhat fewer than were present in the parental callus. Presumably a further round of chromosome elimination is associated with, or is required for plant regeneration (Oberwalder et al 1998). In asymmetric hybrids, the assumption is that the pretreatment (here UV irradiation) given to the donor genome damages the integrity of its genome, and thus makes the donor genome more prone to chromosome elimination.…”

Section: Chromosome Eliminationmentioning

confidence: 99%

Introgression of the heterologous nuclear DNAs and efficacious compositions from Swertia tetraptera Maxim. into Bupleurum scorzonerifolium Willd. via somatic hybridization

et al. 2011

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Swertia tetraptera Maxim. is an important source of secoiridoid glucosides. To produce these pharmacologically valuable compounds heterologously in somatic hybrid cell lines, S. tetraptera protoplasts were irradiated with various doses of UV light and fused with protoplasts from a long-term cell line of Bupleurum scorzonerifolium Willd. This recipient was chosen as the cell line is cytogenetically stable and fast growing; furthermore, protoplasts isolated from the cell line are readily regenerable. From a set of 86 putative hybrid calli, only two were able to regenerate viable green plants. The hybridity of the 19 of the 86 selections was revealed by a combined isozyme and RAPD analysis, supported by a karyotypic study based on genomic in situ hybridization (GISH). Clone I-3 contained 0.014% swertiamarin while the regenerants had 0.069% swertiamarin and 0.409% gentiopicroside while the III-4 plants contained only 0.015% gentiopicroside.

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Asymmetric protoplast fusions between wild species and breeding lines of potato – effect of recipients and genome stability

Cited by 24 publications

References 21 publications

Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl.

Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl.

Interspecific hybrids between Medicago sativa L. and annual Medicago containing Alfafa weevil resistance

Introgression of the heterologous nuclear DNAs and efficacious compositions from Swertia tetraptera Maxim. into Bupleurum scorzonerifolium Willd. via somatic hybridization

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