Asymmetric synergism and heteroencapsidation between two bipartite begomoviruses, tomato leaf curl New Delhi virus and tomato leaf curl Palampur virus

Kanakala, Surapathrudu; Jyothsna, P.; Shukla, Richa; Tiwari, Neha; Veer, B.S.; Swarnalatha, P.; Krishnareddy, M.; Malathi, V. G.

doi:10.1016/j.virusres.2013.03.011

Cited by 23 publications

(15 citation statements)

References 42 publications

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“…2). Comparative results have been described for the same host; for example, after agroinoculation with the ToLCNDV type strain (Kanakala et al 2013) or Potato yellow mosaic virus (Péréfarres et al 2011), it has been suggested that this DNA-A/DNA-B ratio ensured an optimal virus replication.…”

Section: Discussionmentioning

confidence: 97%

Absolute Quantification ofTomato leaf curl New Delhi virusSpain strain, ToLCNDV-ES: Virus Accumulation in a Host-Specific Manner

et al. 2018

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Tomato leaf curl New Delhi virus (ToLCNDV) (family Geminiviridae, genus Begomovirus) has recently been introduced in western Mediterranean countries. Isolates in Spain constitute a new strain, denominated ToLCNDV-ES, that is causing losses in commercial zucchini and melon crops; however, it is also, although less often, detected in commercial tomato crops. We developed a tissue-print hybridization test to detect the two genomic components of the virus and a TaqMan quantitative polymerase chain reaction (qPCR) test to estimate the number of genome copies in plants. qPCR was approximately 104 to 106 times more sensitive than tissue-print hybridization to detect viral genomic DNA-A and DNA-B, respectively. It also detected the virus in more experimentally and naturally ToLCNDV-ES-infected zucchini squash and tomato plants. ToLCNDV-ES DNA-A titers were significantly lower in tomato than in zucchini plants, often falling below the detection limits in the hybridization test. In addition, the DNA-B accumulation was impaired in tomato when compared with zucchini. According to the data obtained in this study, the differences in viral titers of both plant species contribute to explain the dissimilarities in symptom expression, capability of detection, and transmission of the virus.

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Section: Discussionmentioning

confidence: 97%

Absolute Quantification ofTomato leaf curl New Delhi virusSpain strain, ToLCNDV-ES: Virus Accumulation in a Host-Specific Manner

et al. 2018

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“…The extract was clarified at 13,000 × g for 15 min at 4°C. The clarified extract was treated with DNase I (1 U/l) for 30 min at 37°C to remove the viral replicative DNA to ensure that only encapsidated ssDNA served as the template (Kanakala et al, 2013). The plates were washed with 1X PBS three times to remove unbound antibody, and 200 μl aliquots of DNase treated plant and whitefly extract were added into the wells in three replicates.…”

Section: Methodsmentioning

confidence: 99%

Implication of the Whitefly Bemisia tabaci Cyclophilin B Protein in the Transmission of Tomato yellow leaf curl virus

Kanakala

Ghanim

2016

Front. Plant Sci.

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Tomato yellow leaf curl virus (TYLCV) is a single-stranded (ssDNA) begomoviruses that causes severe damage to tomato and several other crops worldwide. TYLCV is exclusively transmitted by the sweetpotato whitefly, Bemisia tabaci in a persistent circulative and propagative manner. Previous studies have shown that the transmission, retention, and circulation of TYLCV in its vector involves interaction with insect and endosymbiont proteins, which aid in the transmission of the virus, or have a protective role in response to the presence of the virus in the insect body. However, only a low number of such proteins have been identified. Here, the role of B. tabaci Cyclophilin B (CypB) in the transmission of TYLCV protein was investigated. Cyclophilins are a large family of cellular prolyl isomerases that have many molecular roles including facilitating protein-protein interactions in the cell. One cyclophilin protein has been implicated in aphid-luteovirus interactions. We demonstrate that the expression of CypB from B. tabaci is altered upon TYLCV acquisition and retention. Further experiments used immunocapture-PCR and co-immunolocalization and demonstrated a specific interaction and colocalization between CypB and TYLCV in the the midgut, eggs, and salivary glands. Membrane feeding of anti-CypB antibodies and TYLCV-infected plants showed a decrease in TYLCV transmission, suggesting a critical role that CypB plays in TYLCV transmission. Further experiments, which used membrane feeding with the CypB inhibitor Cyclosporin A showed decrease in CypB-TYLCV colocalization in the midgut and virus transmission. Altogether, our results indicate that CypB plays an important role in TYLCV transmission by B. tabaci.

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“…The DNA‐A (GenBank accession ) and DNA‐B (GenBank accession ) components of ToLCPalV‐[IN:Punjab:Tom:10] described in Kanakala et al . () represented ToLCPalV.…”

Section: Methodsmentioning

confidence: 99%

Pyramiding Ty‐2 and Ty‐3 genes for resistance to monopartite and bipartite tomato leaf curl viruses of India

et al. 2014

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Breeding resistance to whitefly‐transmitted begomoviruses is an important goal of tomato breeding programmes worldwide. So far, resistance to begomoviruses in tomato has been achieved using wild species, and at least five resistance genes (Ty genes) have been studied. The present study was undertaken to combine Ty‐2 and Ty‐3 and to determine the effect of pyramiding on infection of tomato by three diverse begomovirus species. The diagnostic ability of the markers linked to Ty genes was assessed and marker‐assisted selection was used to develop pyramided tomato lines from the crosses between Ty stocks. Five stable pyramided tomato lines that differ in fruit morphology and yield potential were developed. The horticultural performance of pyramided lines in field trials showed that the yield and horticultural traits are well maintained in the plants. The response of these lines was assessed using agroinoculation and field tests in a disease hotspot. The pyramided lines and Ty‐3‐carrying lines exhibited a high level of resistance to the monopartite and two bipartite begomoviruses tested. The pyramided tomato lines developed in this study could be important genetic resources for sustainable tomato production in areas affected by tomato leaf curl virus disease. The combined results of disease resistance tests also showed that Ty‐3 is critical for achieving broad‐spectrum resistance. The limitations of relying on a single gene and the importance of pyramiding are discussed in the light of available evidence on frequent recombination in begomoviruses.

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Asymmetric synergism and heteroencapsidation between two bipartite begomoviruses, tomato leaf curl New Delhi virus and tomato leaf curl Palampur virus

Cited by 23 publications

References 42 publications

Absolute Quantification ofTomato leaf curl New Delhi virusSpain strain, ToLCNDV-ES: Virus Accumulation in a Host-Specific Manner

Absolute Quantification ofTomato leaf curl New Delhi virusSpain strain, ToLCNDV-ES: Virus Accumulation in a Host-Specific Manner

Implication of the Whitefly Bemisia tabaci Cyclophilin B Protein in the Transmission of Tomato yellow leaf curl virus

Pyramiding Ty‐2 and Ty‐3 genes for resistance to monopartite and bipartite tomato leaf curl viruses of India

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