Abstract. We are observing a disruption in the urban transportation worldwide. The number of cities offering shared-use on-demand mobility services is increasing rapidly. They promise sustainable and affordable personal mobility without a burden of owning a vehicle. Despite growing popularity, on-demand services, such as carsharing, remain niche products due to small scale and rebalancing issues. We are proposing an extension to the traditional carsharing, which is Autonomous Mobility on Demand (AMOD). AMOD provides a one-way carsharing with self-driving electric vehicles. Autonomous vehicles can make the carsharing more attractive to customers as they (i) reduce the operating cost, which is incurred when a manually driven system is unbalanced, and (ii) release people from the burden of driving. This study is built upon our previous work on Autonomous Mobility on Demand (AMOD) systems. Our methodology is simulation-based and we make use of SimMobility, an agent-based microscopic simulation platform. In the current work we focus on the framework for testing different rebalancing policies for the AMOD systems. We compare three different rebalancing methods: (i) no rebalancing, (ii) offline rebalancing, and (iii) online rebalancing. Simulation results indicate that rebalancing reduces the required fleet size and shortens the customers' wait time.