Asynchronous embryo transfer as a tool to understand embryo–uterine interaction in cattle: is a large conceptus a good thing?

Randi, F.; Fernández-Fuertes, Beatriz; McDonald, Michael; Forde, Niamh; Kelly, A. K.; Amorin, Habner Bastos; Lima, E. M.; Morotti, Fábio; Seneda, Marcelo Marcondes; Lonergan, Patrick

doi:10.1071/rd15195

Cited by 43 publications

(49 citation statements)

References 43 publications

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“…Similar data have been reported recently by Randi et al (2015) who transferred multiple day 7 bovine blastocysts to synchronous (day 7) or asynchronous (day 5 or day 9) recipients (n = 10 per recipient). Transfer of day 7 blastocysts to a day 5 uterus resulted in fewer conceptuses surviving (20%) and delayed elongation in those that were recovered.…”

Section: Asynchronous Embryo Transfersupporting

confidence: 75%

“…Given the above results indicating that transfer to an advanced uterus (i.e., uterus ahead of the embryo), which has had longer exposure to P4 results in an advancement in conceptus elongation and that such advanced conceptuses produce more IFNT (Kerbler et al, 1997;Rizos et al, 2012), one could reasonably hypothesize that transfer to an advanced uterus would result in improved pregnancy rates. However, interrogation of data from commercial embryo transfer operations does not support that hypothesis (Wright, 1981;Donaldson, 1985;Hasler et al, 1987;Heyman, 1988;Hasler, 2001;Rodrigues et al, 2003;Randi et al, 2015). For example, in the study of Randi et al (2015), 4749 recipients received a single in vitro produced fresh blastocyst.…”

Section: Asynchronous Embryo Transfermentioning

confidence: 82%

“…However, interrogation of data from commercial embryo transfer operations does not support that hypothesis (Wright, 1981;Donaldson, 1985;Hasler et al, 1987;Heyman, 1988;Hasler, 2001;Rodrigues et al, 2003;Randi et al, 2015). For example, in the study of Randi et al (2015), 4749 recipients received a single in vitro produced fresh blastocyst. Overall pregnancy rate was 43.5%, which is about the norm in such commercial IVF operations.…”

Section: Asynchronous Embryo Transfermentioning

confidence: 82%

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Progesterone and conceptus-derived factors important for conceptus survival and growth

2016

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Progesterone (P4) from the corpus luteum (CL) is critical for the establishment and maintenance of pregnancy and plays a major role in regulating endometrial secretions essential for stimulating and mediating changes in conceptus growth and differentiation throughout early pregnancy in ruminants. Numerous studies have demonstrated an association between elevated P4 and acceleration in conceptus elongation. A combination of in vivo and in vitro experiments found that the effects of P4 on conceptus elongation are indirect and mediated through P4-induced effects in the endometrium. Despite effects on elongation, data on the impact of post-insemination supplementation of P4 on pregnancy rates are conflicting and typically only result in a modest improvement, if any, in fertility. Differences in conceptus length on the same day of gestation would suggest that factors intrinsic to the blastocysts transferred regulate development, at least in part, and would be consistent with the hypothesis that the quality of the oocyte regulates developmental competence. This paper will review recent knowledge on the effect of P4 on conceptus development in cattle and summarize strategies that have been undertaken to manipulate post fertilization P4 concentrations to increase fertility.

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Section: Asynchronous Embryo Transfersupporting

confidence: 75%

Section: Asynchronous Embryo Transfermentioning

confidence: 82%

Section: Asynchronous Embryo Transfermentioning

confidence: 82%

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Progesterone and conceptus-derived factors important for conceptus survival and growth

2016

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“…Transfer of day 7 embryos to a synchronous uterus (day 7) resulted in a pregnancy rate of 47.3%. Transfer to an asynchronous uterus of day 5 (40.8%) or day 8 embryos (41.3%) moderately impacted the pregnancy rate (P < 0.01), but transfer to the uterus 2 days in advance (day 9, 24.4%) or 3 days behind (day 4, 27.0%) reduced (P < 0.001) the pregnancy rate compared with synchronous transfer (Randi et al, 2015). Interestingly, this study emphasized the importance of greater possible synchrony between the embryonic stage and the cycle day of the recipient.…”

Section: Importance Of Recipient Cow Selectionmentioning

confidence: 99%

Cryopreservation of in vitro-produced embryos: challenges for commercial implementation

Sanches¹,

Zangirólamo²,

Silva³

et al. 2017

Anim Reprod

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In the last several years, the high demand for embryo production has resulted in the need to study new methods to make the cryopreservation of bovine embryos produced in vitro more efficient. Despite the advantages offered by in vitro embryo production (IVEP), the major challenge to its greater dissemination is to improve embryonic survival after cryopreservation. Embryos that are produced in vitro are less resistant to cryopreservation compared to those produced in vivo, which is due to the higher accumulation of lipids in their cells, among other factors. In this context, changes in the culture conditions such as the addition of lipolytic chemical substances and the adjustment of fetal calf serum in the medium have been proposed to decrease the lipid amount within the embryos. Several years ago, vitrification allowed good results for in vitro produced (IVP) embryos because of its simplicity, speed and low cost. More recently, another technique applied to simplify the embryo post-thawing rehydration step in vivo, direct transfer (DT), is a strategy that has proven to be of interest in helping to overcome limitations to the cryopreservation of in vitro produced embryos. DT has been performed by commercial laboratories, ensuring good embryo viability after thawing. However, commercial and operational limitations prevent the large-scale use of these techniques. Thus, this review aims to discuss the use of strategies to improve the postcryopreservation survival capacity and the aspects to be overcome so that the cryopreservation of IVP embryos becomes a well-established and commercially applicable technique in addition to presenting new guidelines for embryo transfer (ET) programs from a better selection of recipients.

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“…Once hatched, the blastocyst forms an ovoid-shaped conceptus between days 12-14 and the elongation process begins. Elongation entails rapid proliferation of the conceptus trophectoderm cells, reaching 3-4 mm or more in length by day 14 (Randi et al, 2015), and 25 cm or more in length by day 17. As the embryo elongates, the trophectoderm and endometrial luminal epithelium (LE) become closely apposed, see Spencer et al (2007), for review.…”

Section: Introductionmentioning

confidence: 99%

Embryo maternal immune interactions in cattle

Fair¹

2016

Anim Reprod

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Mammalian embryo implantation requires the priming of the maternal immune system, but, not the provocation. There are many examples of conditions where a disturbed or aberrant immune profile during embryo implantation leads to pregnancy loss. However, these studies are primarily associated with human and mouse species; data is generally limited for cattle and livestock. Most available information centres on the endometrial response to interferon tau (IFNT), a type I antiviral cytokine, which is the maternal recognition factor for cattle and sheep. Interferon tau secretion by the embryo and detection by the dam is critical to corpus luteum (CL) maintenance and pregnancy retention. However, the large volume of bovine endometrial and conceptual transcriptomic data highlights a broader more integral role of the maternal immune system in the establishment of pregnancy in cattle. When taken together with available immunohistochemistry and flow cytometry data from livestock, mouse, and human, a profile of immune cell involvement from ovulation to conception and placentation emerges. The key events of pregnancy establishment in cattle and the involvement of the maternal immune system will be discussed.

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Asynchronous embryo transfer as a tool to understand embryo–uterine interaction in cattle: is a large conceptus a good thing?

Cited by 43 publications

References 43 publications

Progesterone and conceptus-derived factors important for conceptus survival and growth

Progesterone and conceptus-derived factors important for conceptus survival and growth

Cryopreservation of in vitro-produced embryos: challenges for commercial implementation

Embryo maternal immune interactions in cattle

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