Asynchronous replication in oncological patients

Mikhailova, G. F.; Цепенко, В В; Шкаврова, Т. Г.; Голуб, Е В

doi:10.17650/2313-805x-2018-5-1-26-34

Cited by 2 publications

(2 citation statements)

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“…Our study on lymphocytes with asynchronous replication of the TP53 gene both in cancer-free subjects and in patients with solid tumors showed good agreement with the main findings of others [8,24,27]. Thus, our results on the replication timing of biallelically expressed genes in blood lymphocytes from cancer patients [9,28], suggest that AGR can serve as a nonspecific marker of cancer.…”

Section: Discussionsupporting

confidence: 89%

“…In [8], the authors showed that in patients with renal cell carcinomas, the number of lymphocytes with AGR for the TP53 gene and locus 21q22 was 36 and 44%, respectively, while in healthy individuals, the numbers were much lower: 12 and 14%. Subsequently, such studies were carried out with other genes both in healthy controls and in cancer patients [9].…”

Section: Introductionmentioning

confidence: 99%

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Asynchronous DNA Replication of Biallelically Expressed Genes in Human Peripheral Blood Lymphocytes as a Prognostic Sign of Cancer

Цепенко¹,

Шкаврова²,

Cherkesov³

et al. 2021

Sovrem Tehnol Med

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The aim of the study was to identify and quantify lymphocytes with asynchronous replication of the AURKA and TP53 genes in cancer patients versus controls and to assess the diagnostic capabilities of this approach.Materials and Methods. The study was carried out with peripheral blood lymphocytes probed for the AURKA and TP53 genes using the interphase fluorescence in situ hybridization (FISH) method (Vysis, USA and Kreatech, The Netherlands). The control group included 70 people: clinically healthy donors and patients with non-oncological diseases such as gastritis, pancreatitis, chronic calculous cholecystitis, bronchial asthma, peptic ulcer disease, inguinal hernia, arthrosis, myoma, hepatitis, epilepsy, chronic prostatitis, chronic tonsillitis, and rectal adenoma. The group of cancer patients included 219 people with various oncological diseases: gastric cancer (n=68), colorectal cancer (n=30), chronic lymphocytic leukemia (n=52), Hodgkin lymphoma (n=33), and polyneoplasia (n=41).Results. In the control group, the mean frequency of lymphocytes with asynchronous gene replication (AGR) was 22.0±3.4% for AURKA and 18.0±3.2% for TP53; in the group of cancer patients, that was 36.8±4.8 and 28.4±5.1%, respectively. The excessive presence of lymphocytes with the AGR in cancer patients was consistent and statistically significant (p<0.0001). For the AURKA gene, the AGRbased cancer detection showed a sensitivity of 98.6±0.7%, a specificity of 100%, and an accuracy of 98.3±0.8%, and for the TP53 gene -78.6±3.1, 98.5±0.9, and 85.9±2.6%, respectively.Conclusion. This pilot study on lymphocytes with AGR of AURKA and TP53 genes in cancer patients can serve a basis for creating a new molecular cytogenetic technology for detecting malignant neoplasms in humans.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Asynchronous DNA Replication of Biallelically Expressed Genes in Human Peripheral Blood Lymphocytes as a Prognostic Sign of Cancer

Цепенко¹,

Шкаврова²,

Cherkesov³

et al. 2021

Sovrem Tehnol Med

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Asynchronous replication of AURKA and TP53 genes in gastric cancer patients and patients with multiple tumors

et al. 2019

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Background. The correct genome replication is essential for normal cell division to guarantee that genetic information comes changeless through the next cells generations. DNA replication is a strictly regulated and synchronous process and its disturbances could result to mutations appearances. Aberrant time of DNA replication affects on gene expression causes changes of epigenetic modifications and influences on increasing the structural rearrangements leading to enhanced genome disbalance. Replication time failure as asynchronous replication is common for cancerogeneses. The objective of our study was the assessment of asynchronous replication levels in patients with gastric cancer and patients with multiple tumors.Materials and methods. Fluorescence in situ hybridization (FISH) was used for the asynchronous replication of AURKA and TP53 genes analyses. Interphase FISH on lymphocytes of peripheral blood of 37 healthy donors, 19 patients with non-cancer gastrointestinal pathologies, 68 patients with solitary gastric cancer and 39 patients with multiple tumors having gastric cancer and other second synchronous or metachronous tumor was carried out.Results. Values of lymphocytes with asynchronous replication for AURKA were 19.8 ± 0.5 % for control group, 24.7 ± 0.4 % for non-cancer patients, 32.5 ± 0.5 % for gastric cancer patients, 39.5 ± 0.6 % for patients with multiple tumors and 17.3 ± 0.5, 19.5 ± 0.7, 26.1 ± 0.7 and 32.5 ± 0.6 % for TP53 respectively. Differences between cell populations of examined groups had statistical significance with p <0.01 for both studied gene. Also there was statistical difference between gastric cancer patients having distant metastases and gastric cancer patients without metastases for AURKA (34.4 ± 1.0 % vs. 31.7 ± 0.6 %; p = 0.02).Conclusion. High lymphocytes with asynchronous replication level in oncological patients could serve as potential marker of second tumor or possible metastatic process including the earliest stage of it.

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Asynchronous replication in oncological patients

Cited by 2 publications

References 52 publications

Asynchronous DNA Replication of Biallelically Expressed Genes in Human Peripheral Blood Lymphocytes as a Prognostic Sign of Cancer

Asynchronous DNA Replication of Biallelically Expressed Genes in Human Peripheral Blood Lymphocytes as a Prognostic Sign of Cancer

Asynchronous replication of AURKA and TP53 genes in gastric cancer patients and patients with multiple tumors

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