AT-HOOK MOTIF NUCLEAR LOCALIZED (AHL) proteins of ancient origin radiate new functions

Zhang, Weimeng; Cheng, Xiuzhu; Fang, Dongdong; Cao, Jun

doi:10.1016/j.ijbiomac.2022.06.100

Cited by 13 publications

(7 citation statements)

References 127 publications

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“…The AT-hook motif is composed of three amino acids, which is R-G-R. The PCC domain is volatile and can be divided into two types: L-R-S-H and F-T-P-H [ 29 ]. Based on the protein sequence of their conserved domains, the 21 LcAHLs could be divided into two clades: Clade A and Clade B.…”

Section: Resultsmentioning

confidence: 99%

AT-Hook Transcription Factors Show Functions in Liriodendron chinense under Drought Stress and Somatic Embryogenesis

Tang

Zheng³

et al. 2023

Plants

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AT-hook motif nuclear localized (AHL) is a transcription factor that can directly induce plant somatic embryogenesis without adding exogenous hormones. One of its functional domains, the AT-hook motif, has a chromatin-modifying function and participates in various cellular processes, including DNA replication and repair and gene transcription leading to cell growth. Liriodendron chinense (Hemsl.) Sargent is an important ornamental and timber tree in China. However, its low drought-resistant ability further leads to a low natural growth rate of its population. Based on bioinformatics analysis, this study identified a total of 21 LcAHLs in L. chinense. To explore the expression pattern of the AHL gene family under drought and somatic embryogenesis, we performed a systematic analysis including basic characteristics, gene structure, chromosome localization, replication event, cis-acting elements and phylogenetic analyses. According to the phylogenetic tree, the 21 LcAHL genes are divided into three separate clades (Clade I, II, and III). Cis-acting element analysis indicated the involvement of the LcAHL genes in drought, cold, light, and auxin regulation. In the generated drought stress transcriptome, a total of eight LcAHL genes showed increased expression levels, with their expression peaking at 3 h and leveling off after 1 d. Nearly all LcAHL genes were highly expressed in the process of somatic embryogenesis. In this study, we performed a genome-wide analysis of the LcAHL gene family and found that LcAHLs take part in resistance to drought stress and the development of somatic embryos. These findings will provide an important theoretical basis for understanding of the LcAHL gene function.

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Section: Resultsmentioning

confidence: 99%

AT-Hook Transcription Factors Show Functions in Liriodendron chinense under Drought Stress and Somatic Embryogenesis

Tang

Zheng³

et al. 2023

Plants

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“…Identification of AT‐Hook motif nuclear localized (AHL) members as the cis ‐acting element on the SWEET2 promoter uncovered a novel transcriptional regulation of SWEET2 function (Supporting Information S1: Figure ; Figure 3b). The AHL family is an ancient TF family present in most plant species (Zhang et al, 2022; Zhao et al, 2014) and participating in various aspects of plant growth and development, including flower development (Yun et al, 2012), hypocotyl elongation (Zhao et al, 2013), root growth (Širl et al, 2020) and hormone homoeostasis (Matsushita et al, 2007). In Arabidopsis, 29 AHL members have been identified (Zhao et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

The AT‐hook protein AHL29 promotes Bacillus subtilis colonization by suppressing SWEET2‐mediated sugar retrieval in Arabidopsis roots

Wu,

Yu,

Chiu

et al. 2023

Plant Cell & Environment

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Beneficial Bacillus subtilis (BS) symbiosis could combat root pathogenesis, but it relies on root‐secreted sugars. Understanding the molecular control of sugar flux during colonization would benefit biocontrol applications. The SWEET (Sugar Will Eventually Be Exported Transporter) uniporter regulates microbe‐induced sugar secretion from roots; thus, its homologs may modulate sugar distribution upon BS colonization. Quantitative polymerase chain reaction revealed that gene transcripts of SWEET2, but not SWEET16 and 17, were significantly induced in seedling roots after 12 h of BS inoculation. Particularly, SWEET2‐β‐glucuronidase fusion proteins accumulated in the apical mature zone where BS abundantly colonized. Yet, enhanced BS colonization in sweet2 mutant roots suggested a specific role for SWEET2 to constrain BS propagation, probably by limiting hexose secretion. By employing yeast one‐hybrid screening and ectopic expression in Arabidopsis protoplasts, the transcription factor AHL29 was identified to function as a repressor of SWEET2 expression through the AT‐hook motif. Repression occurred despite immunity signals. Additionally, enhanced SWEET2 expression and reduced colonies were specifically detected in roots of BS‐colonized ahl29 mutant. Taken together, we propose that BS colonization may activate repression of AHL29 on SWEET2 transcription that would be enhanced by immunity signals, thereby maintaining adequate sugar secretion for a beneficial Bacillus association.

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“…Ethylene signaling is critical for induced systemic resistance to necrotrophic fungal pathogens, and Brassica napus infected with white mold showed induced ethylene pathway activity based on an analysis of transcriptome activity 48 h post infection [ 7 , 45 ]. In addition, zinc finger motif proteins, endoglucanase, subtilase, PMR5, peroxidase, thionin, xyloglucan, remorin, thaumatin, AT-hook, and lipid transfer proteins have all been implicated in plant defense [ 32 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study (GWAS) of White Mold Resistance in Snap Bean

Arkwazee

Wallace

Hart³

et al. 2022

Genes

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White mold can result in snap bean yield losses of 90 to 100% when field conditions favor the pathogen. A genome-wide association study (GWAS) was conducted to detect loci significantly associated with white mold resistance in a panel of snap bean (Phaseolus vulgaris L.) cultivars. Two populations of snap bean were used in this study. The first population was the BeanCAP (Coordinated Agriculture Project) Snap Bean Diversity Panel (SBDP) (n = 136), and the second population was the Snap Bean Association Panel (SnAP) (n = 378). SBDP was evaluated for white mold reaction in the field in 2012 and 2013, and SnAP was screened in a greenhouse only using the seedling straw test in 2016. Two reference genomes representing the Andean and Middle American centers of domestication were utilized to align the genotyping-by-sequencing (GBS) data. A GWAS was performed using FarmCPU with one principal component after comparing five models. Thirty-four single-nucleotide polymorphisms (SNPs) significantly associated with white mold resistance were detected. Eleven significant SNPs were identified by the seedling straw test, and 23 significant SNPs were identified by field data. Fifteen SNPs were identified within a 100 kb window containing pentatricopeptide repeat (PPR)-encoding genes, and eleven were close to leucine-rich repeat (LRR)-encoding genes, suggesting that these two classes are of outsized importance for snap bean resistance to white mold.

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AT-HOOK MOTIF NUCLEAR LOCALIZED (AHL) proteins of ancient origin radiate new functions

Cited by 13 publications

References 127 publications

AT-Hook Transcription Factors Show Functions in Liriodendron chinense under Drought Stress and Somatic Embryogenesis

AT-Hook Transcription Factors Show Functions in Liriodendron chinense under Drought Stress and Somatic Embryogenesis

The AT‐hook protein AHL29 promotes Bacillus subtilis colonization by suppressing SWEET2‐mediated sugar retrieval in Arabidopsis roots

Genome-Wide Association Study (GWAS) of White Mold Resistance in Snap Bean

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