AT-Hook Transcription Factors Restrict Petiole Growth by Antagonizing PIFs

Favero, David S.; Kawamura, Ayako; Shibata, Michitaro; Takebayashi, Arika; Jung, Jae‐Hoon; Suzuki, Takamasa; Jaeger, Katja E.; Ishida, Takashi; Iwase, Akira; Wigge, Philip A.; Neff, Michael M.; Sugimoto, Keiko

doi:10.1016/j.cub.2020.02.017

Cited by 49 publications

(37 citation statements)

References 124 publications

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“…4 ). This is not surprising considering that several AHLs , including OsAHL1 , AtAHL5, AtAHL10, AtAHL12 , AtAHL16 , AtAHL20 , AtAHL22 , AtAHL27 and AtAHL29, have been reported to exhibit promoter binding capabilities or been shown to confer transcriptional repression or activation of downstream target genes [ 7 , 8 , 10 , 11 , 19 , 24 , 39 , 41 , 42 , 54 ]. In particular, a previous study showed that AtAHL20 is a negative regulator of defenses in Arabidopsis [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…Lee and Seo, [ 24 ] went further and showed that AtAHL27 and AtAHL29 bind YUC9 promoter and suppress gene expression via chromatin modification activities of SWI2/SNF2-RELATED 1 (SWR1) complex. Recently, Favero et al [ 8 ] showed that AtAHL29 binds to PIF-targeted loci to reduce binding of PIF to these regions, thereby inhibiting transcriptional activation of growth promoting genes in Arabidopsis petioles. We hypothesize that AtAHL20 may also bind FT promoter elements and suppress its expression, perhaps individually or as part of complex.…”

Section: Discussionmentioning

confidence: 99%

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Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expression

et al. 2020

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Background The 29-member Arabidopsis AHL gene family is classified into three main classes based on nucleotide and protein sequence evolutionary differences. These differences include the presence or absence of introns, type and/or number of conserved AT-hook and PPC domains. AHL gene family members are divided into two phylogenetic clades, Clade-A and Clade-B. A majority of the 29 members remain functionally uncharacterized. Furthermore, the biological significance of the DNA and peptide sequence diversity, observed in the conserved motifs and domains found in the different AHL types, is a subject area that remains largely unexplored. Results Transgenic plants overexpressing AtAHL20 flowered later than the wild type under both short and long days. Transcript accumulation analyses showed that 35S:AtAHL20 plants contained reduced FT, TSF, AGL8 and SPL3 mRNA levels. Similarly, overexpression of AtAHL20’s orthologue in Camelina sativa, Arabidopsis’ closely related Brassicaceae family member species, conferred a late-flowering phenotype via suppression of CsFT expression. However, overexpression of an aberrant AtAHL20 gene harboring a missense mutation in the AT-hook domain’s highly conserved R-G-R core motif abolished the late-flowering phenotype. Data from targeted yeast-two-hybrid assays showed that AtAHL20 interacted with itself and several other Clade-A Type-I AHLs which have been previously implicated in flowering-time regulation: AtAHL19, AtAHL22 and AtAHL29. Conclusion We showed via gain-of-function analysis that AtAHL20 is a negative regulator of FT expression, as well as other downstream flowering time regulating genes. A similar outcome in Camelina sativa transgenic plants overexpressing CsAHL20 suggest that this is a conserved function. Our results demonstrate that AtAHL20 acts as a photoperiod-independent negative regulator of transition to flowering.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expression

et al. 2020

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“…AtAHL22 binds with the AT-rich sequence in the FLOWERING LOCUS by recruiting histone deacetylases (HDAC) and regulates flowering time (Yun et al, 2012). The SUPPRESSOR OF PHYTOCHROME B4-#3 (SOB3) coding an AT-hook protein restricts petiole elongation in Arabidopsis (Favero et al, 2020). Besides, several AHL genes are known to play crucial roles in abiotic and biotic stresses.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of AHL gene family and their expression under drought stress and ABA treatment in Populus trichocarpa

Wang

Leng

Yang

et al. 2021

PeerJ

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The AT-hook motif nuclear-localized (AHL) family is a plant transcription factor family, which plays an important role in growth and development and stress responses. We identified and analyzed 37 AHL genes in poplar (Populus trichocarpa). Phylogenetic analysis classified the PtrAHL members into three subfamilies based on their conserved domain. All PtrAHL paralogous pairs evolved under purifying selection. The promoter analysis revealed the presence of stress-related and phytohormone-related cis-elements of the PtrAHL genes. Our analysis of the tissue-specific expression pattern of PtrAHL genes indicated their significance in tissue and organ development. Network-based prediction suggested that PtrAHL genes may interact with histone deacetylases (HDAC) and participate in the development of organs, such as roots. Drought negatively impacts plant growth and development. ABA is produced under osmotic stress condition, and it takes an important part in the stress response and tolerance of plants. Real-time quantitative PCR (qRT-PCR) showed that PtrAHL genes were induced by drought stress and ABA treatment. These insights into the expression of PtrAHL genes under stress provide a basis for PtrAHL gene functional analysis. Our study will help develop new breeding strategies to improve drought tolerance in poplar.

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“…(2017) went further and showed that AtAHL27 and AtAHL29 bind YUC9 promoter and suppress gene expression via chromatin modi cation activities of SWI2/SNF2-RELATED 1 (SWR1) complex. Recently, Favero et al (2020) showed that AtAHL29 binds to PIF-targeted loci to reduce binding of PIF to these regions, thereby inhibiting transcriptional activation of growth promoting genes in Arabidopsis petioles. We hypothesize that AtAHL20 may also bind FT promoter elements and suppress its expression, perhaps individually or as part of complex.…”

Section: Atahl20 Is a Repressor Of Ft Expressionmentioning

confidence: 99%

Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expression

Tayengwa¹,

Sharma-Koirala

Pierce³

et al. 2020

Preprint

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Background The 29-member Arabidopsis AHL gene family is classified into three main classes based on nucleotide and protein sequence evolutionary differences. These differences include the presence or absence of introns, type and/or number of conserved AT-hook and PPC domains. AHL gene family members are divided into two phylogenetic clades, Clade-A and Clade-B. A majority of the 29 members remain functionally uncharacterized. Furthermore, the biological significance of the DNA and peptide sequence diversity, observed in the conserved motifs and domains found in the different AHL types, is a subject area that remains largely unexplored. Results Transgenic plants overexpressing AtAHL20 flowered later than the wild type. Transcript accumulation analyses showed that 35S:AtAHL20 plants contained reduced FT, TSF, AGL8 and SPL3 mRNA levels. Similarly, overexpression of AtAHL20’s orthologue in Camelina sativa, Arabidopsis’ closely related Brassicaceae family member species, conferred a late-flowering phenotype via suppression of CsFT expression. In addition, 35S:AtAHL20 seedlings exhibited suppressed hypocotyl length and enhanced water stress tolerance. However, overexpression of an aberrant AtAHL20 gene harboring a missense mutation in the AT-hook domain’s highly conserved R-G-R core motif abolished the late-flowering phenotype. Data from targeted yeast-two-hybrid assays showed that AtAHL20 interacted with itself and several other Clade-A Type-I AHLs which have been previously implicated in flowering-time regulation: AtAHL22, AtAHL27 and AtAHL29. Conclusion We showed via gain-function analysis that AtAHL20 is a negative regulator of FT expression, as well as other downstream flowering time regulating genes. A similar outcome in Camelina sativa transgenic plants overexpressing CsAHL20 suggest that this is a conserved function. Additionally, overexpression of AtAHL20 resulted in shorter hypocotyls and enhanced drought stress tolerance compared to wild-type plants. Our results demonstrate that AtAHL20 is a negative regulator of transition to flowering and hypocotyl elongation, but a positive regulator of drought stress tolerance.

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AT-Hook Transcription Factors Restrict Petiole Growth by Antagonizing PIFs

Cited by 49 publications

References 124 publications

Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expression

Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expression

Comprehensive analysis of AHL gene family and their expression under drought stress and ABA treatment in Populus trichocarpa

Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expression

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