At least two genetically distinct large Babesia species infective to sheep and goats in China

Liu, A.H.; Yin, Hong; Guan, Guiquan; Schnittger, L.; Liu, Z.J.; Ma, Miling; Dang, Zhisheng; Liu, J.L.; Ren, Qiaoyun; Bai, Quan; Ahmed, Jabbar S.; Luo, Jianxun

doi:10.1016/j.vetpar.2007.03.032

Cited by 95 publications

(68 citation statements)

References 10 publications

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“…Kashi 1 (AY726556) also identified from a bovine in China (27) and Babesia sp. Xinjiang-2005 (DQ159073), which often leads to clinically inapparent infection in ovines (18).…”

Section: Resultsmentioning

confidence: 99%

Identification of a Novel Babesia sp. from a Sable Antelope ( Hippotragus niger Harris, 1838)

et al. 2008

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Babesiosis in a sable antelope (Hippotragus niger Harris, 1838) was first reported in 1930; the parasite was named Babesia irvinesmithi. Recently, specimens from an adult sable that presented with a sudden onset of disease and that subsequently died during immobilization were submitted for molecular characterization. Microscopic examination of thin blood smears revealed the presence of small piroplasms. DNA was extracted from blood samples; the V4 variable region of the 18S rRNA gene was amplified and analyzed using the reverse line blot (RLB) assay. Amplicons did not hybridize with any of the Babesia or Theileria species-specific probes present on the blot and hybridized only with a Babesia or Theileria genus-specific probe, suggesting the presence of a novel species. The full-length 18S rRNA gene sequence was obtained and aligned with published sequences of related genera, and phylogenetic trees were constructed. Sequence similarity analyses indicated that a Babesia species, designated Babesia sp. (sable), was present. The sequence showed its highest similarity to B. orientalis and to an unnamed Babesia species previously detected in bovine samples. The latter was later established to be Babesia occultans. A Babesia sp. (sable)-specific RLB oligonucleotide probe was designed and used to screen 200 South African sable samples, but so far, no other sample has been found to be positive for the presence of Babesia sp. (sable) DNA. In summary, we identified a novel piroplasm parasite from a sable antelope that died from an unknown illness. While the parasite was observed in blood smears, there is no direct evidence that it was the cause of death.

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“…Kashi 1 (AY726556) also identified from a bovine in China (27) and Babesia sp. Xinjiang-2005 (DQ159073), which often leads to clinically inapparent infection in ovines (18).…”

Section: Resultsmentioning

confidence: 99%

Identification of a Novel Babesia sp. from a Sable Antelope ( Hippotragus niger Harris, 1838)

et al. 2008

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“…A. ovis has been found to be distributed over 44 counties in Gansu Province, with infection rates of 30.1 to 60.8% (16). Ovine Theileria and Babesia infections have also been found in regions of Gansu Province in recent years (13,23). It is difficult to differentiate between Piroplasma and Anaplasma pathogens on the basis of clinical signs and microscopic examination, and a rapid diagnostic method, LAMP, was therefore developed in the present study.…”

Section: Discussionmentioning

confidence: 98%

Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Detection of Anaplasma ovis

Liu

Sun

et al. 2011

J Clin Microbiol

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Anaplasma ovis is an intraerythrocytic rickettsial pathogen of small ruminants. Loop-mediated isothermal amplification (LAMP) is a nucleic acid detection method in which the target DNA can be efficiently amplified with high specificity and sensitivity under isothermal conditions. In this study, a LAMP method was developed for the specific detection of A. ovis, using LAMP primers designed on the basis of the major surface protein 4 gene. LAMP was performed at 65°C for 30 min. Its specificity was confirmed by successful amplification of several A. ovis isolates and through EcoRI restriction analysis of LAMP products. No cross-reactivity with the A. marginale Lushi isolate, Mycoplasma mycoides subsp. capri, Chlamydophila psittaci, Theileria ovis, T. luwenshuni, T. uilenbergi, or the Babesia sp. Xinjiang isolate was observed. Detection using the LAMP method was compared with that using conventional PCR in 227 field samples; LAMP demonstrated a sensitivity of 95.45%. In summary, LAMP is a specific, sensitive, and rapid test for the diagnosis of A. ovis infection, with the potential to be standardized as a detection method for A. ovis in areas of endemicity.

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“…Xinjiang, originally detected from a batch of mixed Rhipicephalus sanguineus and Hyalomma a. anatolicum ticks from Kashi, Xinjiang province, China, was described as often leading to clinically inapparent infection in sheep (Liu et al, 2007). The double pyriform parasites measure 3.0-4.0 μm × 1.1-2.1 μm (Guan et al, 2001) and this is therefore considered to be a large Babesia (Liu et al, 2007). Rhipicephalus sanguineus or H. a. anatolicum ticks are suspected to be the transmission vector of Babesia sp.…”

Section: The Identification Of Babesia Spp In Giraffe and Roan Antelopementioning

confidence: 99%

“…Rhipicephalus sanguineus or H. a. anatolicum ticks are suspected to be the transmission vector of Babesia sp. Xinjiang (Liu et al, 2007) and, while the vector of the giraffe/roan Babesia remains unknown, Rhipicephalus appendiculatus was among the ticks collected from giraffe 0105. Additional molecular data, as well as information on the tick vectors, host ranges and other biological properties, are required to clarify whether Babesia sp.…”

Section: The Identification Of Babesia Spp In Giraffe and Roan Antelopementioning

confidence: 99%

Identification of novel Babesia and Theileria species in South African giraffe (Giraffa camelopardalis, Linnaeus, 1758) and roan antelope (Hippotragus equinus, Desmarest 1804)

Oosthuizen

Allsopp

Troskie

et al. 2009

Veterinary Parasitology

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At least two genetically distinct large Babesia species infective to sheep and goats in China

Cited by 95 publications

References 10 publications

Identification of a Novel Babesia sp. from a Sable Antelope ( Hippotragus niger Harris, 1838)

Identification of a Novel Babesia sp. from a Sable Antelope ( Hippotragus niger Harris, 1838)

Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Detection of Anaplasma ovis

Identification of novel Babesia and Theileria species in South African giraffe (Giraffa camelopardalis, Linnaeus, 1758) and roan antelope (Hippotragus equinus, Desmarest 1804)

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