At the Dawn of Polytherapy for Laminitis Treatment- Novel Approach to an the Old Problems

Kornicka, Katarzyna; Galuppo, Larry D.; Marycz, Krzysztof

doi:10.1007/s12015-021-10174-2

Cited by 1 publication

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“…Liver insulin resistance is an inseparable component of EMS, which is currently one of the most frequent endocrine disorders among horses. One of the potential therapeutic approaches proposed by our group includes inhibition of protein-tyrosine phosphatase PTP1B [ 14 , 15 , 33 ], by systemic application of MSI-1436 and/or systemic administration of equine liver progenitor cells (Eq_HPCs). Here, for the first time, we characterized a model of Eq_HPCs obtained from the guided differentiation of adipose-derived stromal cells (Eq_ASCs), that might serve as a future reliable and accessible therapeutic tool for EMS treatment, and be considered an appropriate alternative to autologous hepatocytes transplantation.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Generated Equine Hepatic-Like Progenitor Cells as a Novel Potent Cell Pool for Equine Metabolic Syndrome (EMS) Treatment

Marycz

Bourebaba

Serwotka-Suszczak

et al. 2023

Stem Cell Rev and Rep

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Equine metabolic syndrome (EMS) is recognized as one of the leading cause of health threatening in veterinary medicine worldwide. Recently, PTP1B inhibition has been proposed as an interesting strategy for liver insulin resistance reversion in both equines and humans, however as being a multifactorial disease, proper management of EMS horses further necessities additional interventional approaches aiming at repairing and restoring liver functions. In this study, we hypothesized that in vitro induction of Eq_ASCs hepatogenic differentiation will generate a specialized liver progenitor-like cell population exhibiting similar phenotypic characteristics and regenerative potential as native hepatic progenitor cells. Our obtained data demonstrated that Eq_ASCs-derived liver progenitor cells (Eq_HPCs) displayed typical flattened polygonal morphology with packed fragmented mitochondrial net, lowered mesenchymal CD105 and CD90 surface markers expression, and significant high expression levels of specific hepatic lineage genes including PECAM-1, ALB, AFP and HNF4A. therewith, generated Eq_HPCs exhibited potentiated stemness and pluripotency markers expression (NANOG, SOX-2 and OCT-4). Hence, in vitro generation of hepatic progenitor-like cells retaining high differentiation capacity represents a promising new approach for the establishment of cell-based targeted therapies for the restoration of proper liver functions in EMS affected horses. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%