AtCAND1, A HEAT-Repeat Protein That Participates in Auxin Signaling in Arabidopsis

Cheng, Youfa; Dai, Xinhua; Zhao, Yunde

doi:10.1104/pp.104.044495

Cited by 87 publications

(79 citation statements)

References 36 publications

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“…17 Similar effects of CAND1 deficiency on other SCF substrates have been reported. 28,29 However, when we examined the silencing effects of CAND1 deficiency on Drosophila cyclin E, we surprisingly did not see a detectable change in the protein level of dmcyclin E (Fig. 1A).…”

Section: Resultsmentioning

confidence: 99%

Involvement of CUL4 Ubiquitin E3 Ligases in Regulating CDK Inhibitors Dacapo/p27Kip1 and Cyclin E Degradation

Higa¹,

Yang²,

Zheng³

et al. 2005

Cell Cycle

107

136

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Section: Resultsmentioning

confidence: 99%

Involvement of CUL4 Ubiquitin E3 Ligases in Regulating CDK Inhibitors Dacapo/p27Kip1 and Cyclin E Degradation

Higa¹,

Yang²,

Zheng³

et al. 2005

Cell Cycle

107

136

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“…XopN is the first pathogen molecule thought to mimic this eukaryotic fold. The Arabidopsis genome contains many proteins with HEAT repeats including protein phosphatase 2A PR65͞A regulatory subunits (41); several homologs of the nuclear transport protein importin-␤ (42); and AtCAND1, which is involved in the regulation of auxin signaling (43). We speculate that XopN may use HEAT repeat structures to modulate plant signal transduction by mimicking or interfering with proteins containing these repeats.…”

Section: Discussionmentioning

confidence: 99%

A genetic screen to isolate type III effectors translocated into pepper cells during Xanthomonas infection

Roden

Belt

Ross

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

124

112

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The bacterial pathogen Xanthomonas campestris pv. vesicatoria (Xcv) uses a type III secretion system (TTSS) to translocate effector proteins into host plant cells. The TTSS is required for Xcv colonization, yet the identity of many proteins translocated through this apparatus is not known. We used a genetic screen to functionally identify Xcv TTSS effectors. A transposon 5 (Tn5)-based transposon construct including the coding sequence for the Xcv AvrBs2 effector devoid of its TTSS signal was randomly inserted into the Xcv genome. Insertion of the avrBs2 reporter gene into Xcv genes coding for proteins containing a functional TTSS signal peptide resulted in the creation of chimeric TTSS effector::AvrBs2 fusion proteins. Xcv strains containing these fusions translocated the AvrBs2 reporter in a TTSS-dependent manner into resistant BS2 pepper cells during infection, activating the avrBs2-dependent hypersensitive response (HR). We isolated seven chimeric fusion proteins and designated the identified TTSS effectors as Xanthomonas outer proteins (Xops). Translocation of each Xop was confirmed by using the calmodulin-dependent adenylate cydase reporter assay. Three xop genes are Xanthomonas spp.-specific, whereas homologs for the rest are found in other phytopathogenic bacteria. XopF1 and XopF2 define an effector gene family in Xcv. XopN contains a eukaryotic protein fold repeat and is required for full Xcv pathogenicity in pepper and tomato. The translocated effectors identified in this work expand our knowledge of the diversity of proteins that Xcv uses to manipulate its hosts.bacterial plant pathogenesis ͉ virulence proteins

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“…In many cases, slowed degradation of the mutant protein was experimentally verified (Timpte et al 1994;Leyser et al 1996;Rouse et al 1998;Nagpal et al 2000;Tian et al 2002;Tatematsu et al 2004;Yang et al 2004). Other auxin response mutants include plants with defective subunits of the SCF ubiquitin E3 ligase or mutations that cause misregulation of SCF activity/ assembly (Ruegger et al 1998;Gray et al 2003;Hellmann et al 2003;Cheng et al 2004;Chuang et al 2004;Feng et al 2004;Dharmasiri et al 2005b;Quint et al 2005;Alonso-Peral et al 2006;Walsh et al 2006;Moon et al 2007;Woodward et al 2007).…”

mentioning

confidence: 99%

Isolation and Characterization ofcul1-7, a Recessive Allele ofCULLIN1That Disrupts SCF Function at the C Terminus of CUL1 inArabidopsis thaliana

Gilkerson

Brown³

et al. 2009

Genetics

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Many aspects of plant biology depend on the ubiquitin proteasome system for degradation of regulatory proteins. Ubiquitin E3 ligases confer substrate specificity in this pathway, and SCF-type ligases comprise a major class of E3s. SCF ligases have four subunits: SKP1, CUL1, RBX1, and an F-box protein for substrate recognition. The Aux/IAAs are a well-characterized family of SCF substrates in plants. Here, we report characterization of a mutant isolated from a genetic screen in Arabidopsis thaliana designed to identify plants defective in degradation of an Aux/IAA fusion protein, Aux/IAA1-luciferase (IAA1-LUC). This mutant exhibited fourfold slower IAA1-LUC degradation compared with the progenitor line, and seedlings displayed altered auxin responses. Experiments identified the mutant as an allele of CUL1, named cul1-7. The cul1-7 mutation affects the C terminus of the protein, results in reduced cul1-7 levels, and interferes with RBX1 interaction. cul1-7 seedlings are defective in degradation of an endogenous SCF substrate, Repressor of ga1-3 (RGA), and have altered responses to gibberellins. cul1-7 seedlings exhibit slower degradation of the light-labile red/far-red photoreceptor phytochrome A and are photomorphogenic in the dark. This mutation represents the first reported allele of CUL1 to directly affect subunit interactions at the CUL1 C terminus.

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AtCAND1, A HEAT-Repeat Protein That Participates in Auxin Signaling in Arabidopsis

Cited by 87 publications

References 36 publications

Involvement of CUL4 Ubiquitin E3 Ligases in Regulating CDK Inhibitors Dacapo/p27Kip1 and Cyclin E Degradation

Involvement of CUL4 Ubiquitin E3 Ligases in Regulating CDK Inhibitors Dacapo/p27Kip1 and Cyclin E Degradation

A genetic screen to isolate type III effectors translocated into pepper cells during Xanthomonas infection

Isolation and Characterization ofcul1-7, a Recessive Allele ofCULLIN1That Disrupts SCF Function at the C Terminus of CUL1 inArabidopsis thaliana

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