AtDSEL, an Arabidopsis cytosolic DAD1-like acylhydrolase, is involved in negative regulation of storage oil mobilization during seedling establishment

Kim, Eun Yu; Seo, Young Sam; Kim, Woo Taek

doi:10.1016/j.jplph.2011.03.004

Cited by 22 publications

(11 citation statements)

References 25 publications

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“…Therefore, we hypothesized that Arabidopsis SRPs are also involved in LD development. LDs are abundant in the dry-seed stage, and their numbers gradually reduce during germination and seedling establishment in Arabidopsis (Eastmond, 2006;Kim et al, 2011). We analyzed the size and number of cellular LDs in the 6-d-old seedlings of the SRP over-expressors and 35S:SRP2-RNAi/srp1srp3 triple mutant plants by transmission electron microscopy (TEM).…”

Section: Srps Play Positive Roles In the Biogenesis Of Ldsmentioning

confidence: 99%

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Arabidopsis Small Rubber Particle Protein Homolog SRPs Play Dual Roles as Positive Factors for Tissue Growth and Development and in Drought Stress Responses

et al. 2016

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Lipid droplets (LDs) act as repositories for fatty acids and sterols, which are used for various cellular processes such as energy production and membrane and hormone synthesis. LD-associated proteins play important roles in seed development and germination, but their functions in postgermination growth are not well understood. Arabidopsis (Arabidopsis thaliana) contains three SRP homologs (SRP1, SRP2, and SRP3) that share sequence identities with small rubber particle proteins of the rubber tree (Hevea brasiliensis). In this report, the possible cellular roles of SRPs in postgermination growth and the drought tolerance response were investigated. Arabidopsis SRPs appeared to be LD-associated proteins and displayed polymerization properties in vivo and in vitro. SRP-overexpressing transgenic Arabidopsis plants (35S:SRP1, 35S:SRP2, and 35S:SRP3) exhibited higher vegetative and reproductive growth and markedly better tolerance to drought stress than wild-type Arabidopsis. In addition, constitutive over-expression of SRPs resulted in increased numbers of large LDs in postgermination seedlings. In contrast, single (srp1, 35S:SRP2-RNAi, and srp3) and triple (35S:SRP2-RNAi/srp1srp3) loss-of-function mutant lines exhibited the opposite phenotypes. Our results suggest that Arabidopsis SRPs play dual roles as positive factors in postgermination growth and the drought stress tolerance response. The possible relationships between LD-associated proteins and the drought stress response are discussed.Environmental stresses, including drought, high salinity, oxidative stress, and unfavorable temperatures, profoundly affect the growth and development of higher plants. Because of their sessile life cycle, plants have developed self-protective mechanisms to increase their tolerance to short-and long-term stresses by triggering diverse sets of signal transduction pathways and activating stress-responsive genes. The genetic and cellular mechanisms in response to abiotic stress have been widely documented in higher plants (Shinozaki and

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Section: Srps Play Positive Roles In the Biogenesis Of Ldsmentioning

confidence: 99%

“…For TEM, hypocotyl tissues of 6-d-old seedlings were fixed in 2.5% (v/v) glutaraldehyde, 4% (v/v) formaldehyde, and 100 mM phosphate buffer, pH 7.2, as described by Kim et al (2011). Thin sections of tissues were prepared using an ultra-microtome (Leica, Vienna, Austria).…”

Section: Sem and Temmentioning

confidence: 99%

Arabidopsis Small Rubber Particle Protein Homolog SRPs Play Dual Roles as Positive Factors for Tissue Growth and Development and in Drought Stress Responses

et al. 2016

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“…Then, the new ester between the serine and the FA is hydrolyzed. Examples for characterized GXSXG-lipases in Arabidopsis are the MAG and 1,3-DAG lipase AtDESL (Kim et al, 2011), and a number of MAG lipases (Kim et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the enzymatic activity and physiological function of the lipid droplet‐associated triacylglycerol lipase AtOBL1

Müller¹,

Ischebeck²

2017

New Phytologist

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Similar to seeds, pollen tubes contain lipid droplets that store triacylglycerol (TAG), but the fate of this TAG as well as the enzymes involved in its breakdown are unknown. Therefore, two potential TAG lipases from tobacco and Arabidopsis, NtOBL1 (Oil body lipase 1) and AtOBL1, were investigated, especially with respect to their importance for pollen tube growth. We expressed NtOBL1 and AtOBL1 as fluorescent fusion proteins to study their localization by confocal microscopy. Furthermore, we overexpressed AtOBL1 in Nicotiana benthamiana leaves to characterize it enzymatically. The obl1 mutant was studied in respect to its pollen tube growth in vivo and its seed germination. Both NtOBL1 and AtOBL1 localized to lipid droplets. AtOBL1 was abundant in pollen tubes and seedlings, and acted as a lipase on TAG, diacylglycerol and 1-monoacylglycerol at a pH optimum of 5.5. The obl1 mutant was hampered in pollen tube growth, whereas seedling establishment was not affected under optimal conditions, even though AtOBL1 accounted for a major lipase activity in seeds. TAG could be a direct precursor for the synthesis of membrane lipids in pollen tubes and proteins of the OBL family involved in the flux of acyl groups.

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“…The DEFECTIVE IN ANTHER DEHISCIENCE 1 (DAD1) (At2g44810) was originally identified as an Arabidopsis PLA1 that catalysed the initial step for jasmonic acid production in chloroplasts (Ishiguro et al , 2001). The DAD1-like acylhydrolase family consists of 12 members, all of which contain sn -1-specific acylhydrolase activity, and is further divided into three subgroups based on predicted subcellular localizations (Beisson et al , 2003; Ryu, 2004; Seo et al , 2008, 2009; Kim et al , 2011). Seven proteins, including DAD1, belong to class I, which is typified by a putative N-terminal chloroplast-targeting signal, while four cytosolic proteins belong to class II.…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis sn-1-specific mitochondrial acylhydrolase AtDLAH is positively correlated with seed viability

Seo

Kim

2011

Journal of Experimental Botany

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Lipid-derived molecules produced by acylhydrolases play important roles in the regulation of diverse cellular functions in plants. In Arabidopsis, the DAD1-like phospholipase A1 family consists of 12 members, all of which possess a lipase 3 domain. In this study, the biochemical and cellular functions of AtDLAH, an Arabidopsis thaliana DAD1-like acylhydrolase, were examined. Bacterially expressed AtDLAH contained phospholipase A1 activity for catalysing the hydrolysis of phospholipids at the sn-1 position. However, AtDLAH displayed an even stronger preference for 1-lysophosphatidylcholine, 1-monodiacylglycerol, and phosphatidic acid, suggesting that AtDLAH is a sn-1-specific acylhydrolase. The AtDLAH gene was highly expressed in young seedlings, and its encoded protein was exclusively localized to the mitochondria. AtDLAH-overexpressing transgenic seeds (35S:AtDLAH) were markedly tolerant to accelerated-ageing treatment and thus had higher germination percentages than wild-type seeds. In contrast, the atdlah loss-of-function knockout mutant seeds were hypersusceptible to accelerated-ageing conditions. The 35S:AtDLAH seeds, as opposed to the atdlah seeds, exhibited a dark red staining pattern following tetrazolium treatment under both normal and accelerated-ageing conditions, suggesting that AtDLAH expression is positively correlated with seed viability. The enhanced viability of 35S:AtDLAH seeds was accompanied by more densely populated epidermal cells, lower levels of accumulated lipid hydroperoxides, and higher levels of polar lipids as compared with wild-type and atdlah mutant seeds. These results suggest that AtDLAH, a mitochondrial-localized sn-1-specific acylhydrolase, plays an important role in Arabidopsis seed viability.

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AtDSEL, an Arabidopsis cytosolic DAD1-like acylhydrolase, is involved in negative regulation of storage oil mobilization during seedling establishment

Cited by 22 publications

References 25 publications

Arabidopsis Small Rubber Particle Protein Homolog SRPs Play Dual Roles as Positive Factors for Tissue Growth and Development and in Drought Stress Responses

Arabidopsis Small Rubber Particle Protein Homolog SRPs Play Dual Roles as Positive Factors for Tissue Growth and Development and in Drought Stress Responses

Characterization of the enzymatic activity and physiological function of the lipid droplet‐associated triacylglycerol lipase AtOBL1

The Arabidopsis sn-1-specific mitochondrial acylhydrolase AtDLAH is positively correlated with seed viability

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