Atelocollagen-templated fabrication of tangled fibrous silica

Nojima, Tatsuya; Suzuki, Seiya; Iyoda, Tomokazu

doi:10.1039/c6tb01770c

Cited by 2 publications

(2 citation statements)

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“…Much work has been done for silica-collagen hybrid materials, owing to their use in biomedical applications. − These hybrid materials can be evaluated from the sol–gel reaction in the presence of collagen and/or gelatin in aqueous media. − The attractive interaction between silica and collagen plays an important role for the method; however, little work has been done on the interaction. Collagen is the most abundant protein in mammals with a triple helical structure, , which unfolds in water at high temperatures.…”

Section: Introductionmentioning

confidence: 99%

Complex Formation of Silica Nanoparticles with Collagen: Effects of the Conformation of Collagen

2020

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Negatively charged Ludox silica nanoparticles (SiNPs) form a complex with atelocollagen (AC) in acidic buffers (pH = 4 or 3). AC is a low-immunogenic derivative of collagen obtained by the removal of N- and C-terminal telopeptide components. Mixed solutions of negatively charged SiNPs and AC were turbid, while positively charged SiNPs (Ludox CL) did not form a complex with AC in pH 4 buffer, indicating that electrostatic attraction is the dominant force to form the complex. Small-angle X-ray scattering (SAXS) and circular dichroism (CD) measurements were made for AC and Ludox LS (or CL) solutions in acetate buffer (pH 4.0) and citrate buffer (pH 3.0). The CD data showed that the stability of the triple helical structure of AC in the buffers is not affected by the complexation. The resulting complex consisting of triple helical AC and SiNPs did not influence the SAXS profile except for the lowest q region investigated. On the contrary, different scattering profiles were observed for the single chain AC and SiNP mixture indicating densely packed SiNPs in the complex. This scattering behavior was fairly explained in terms of the sticky hard sphere model (SHSM). This AC conformation-dependent complexation may be because of the hydrogen bonding interaction between the single chain AC and SiNPs. The temperature-induced change of the complex formation can be applied for thermoresponsive hybrid materials.

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Section: Introductionmentioning

confidence: 99%

Complex Formation of Silica Nanoparticles with Collagen: Effects of the Conformation of Collagen

2020

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“…The development of protein-based materials with excellent mechanical strength and functional properties is a major goal in materials science. Fibrous proteins, such as elastins, keratins, collagens, cotton silks and spider dragline silks, are widely used to create materials, [1][2][3] whereas soluble globular proteins are less commonly used.…”

Section: Introductionmentioning

confidence: 99%

Egg white-based strong hydrogel via ordered protein condensation

Nojima

Iyoda

2018

NPG Asia Mater

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Despite the well-known thermal gelation property of egg white and its wide use in the food industry, the applications of gelled egg white in materials science are limited owing to the brittleness caused by the network structure of randomly associated denatured proteins. In this study, a gel material with high mechanical strength was fabricated from crude egg white proteins. Fluid materials containing orderly condensed proteins at regular intervals were prepared by addition of ionic surfactants to an egg white protein solution. The initiation of the heat denaturation of proteins from this ordered state with spatial confinement resulted in the formation of hydrogel materials with high mechanical strength. The maximum compressive fracture strength of the fabricated egg white-based hydrogel was 34.5 MPa, which is 150-fold higher than that of gelled egg white prepared by boiling and comparable to that of strong chemically synthesized hydrogels. Chemical modification of sulfhydryl groups of proteins and treatment with a denaturant revealed that denatured polypeptide chains were covalently linked by disulfide bonds and noncovalently associated; these findings showed that the high mechanical strength of our material is due to the synergic effects of both covalent and non-covalent networks.

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Atelocollagen-templated fabrication of tangled fibrous silica

Abstract: An electrostatic complex of protein and ethylenediamine works as a catalytic template for the fabrication of structured silica and other metal oxides.

Cited by 2 publications

References 29 publications

Complex Formation of Silica Nanoparticles with Collagen: Effects of the Conformation of Collagen

Complex Formation of Silica Nanoparticles with Collagen: Effects of the Conformation of Collagen

Egg white-based strong hydrogel via ordered protein condensation

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