Atf1-Pcr1-M26 Complex Links Stress-activated MAPK and cAMP-dependent Protein Kinase Pathways via Chromatin Remodeling of cgs2+

Davidson, Mari K.; Shandilya, Harish; Hirota, Kouji; Ohta, Kunihiro; Wahls, Wayne P.

doi:10.1074/jbc.m409079200

Cited by 45 publications

(69 citation statements)

References 49 publications

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“…It has also been reported that the Atf1/Pcr1 heterodimer functions as both an inducer and a repressor of chromatin remodeling at the cgs2 promoter (9). According to our data, Atf1 and Pcr1 down-regulate the basal transcription of 4% and 6% of the whole S. pombe genome, respectively.…”

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confidence: 51%

Transcription Factors Pcr1 and Atf1 Have Distinct Roles in Stress- and Sty1-Dependent Gene Regulation

et al. 2008

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The mitogen-activated protein kinase Sty1 is essential for the regulation of transcriptional responses that promote cell survival in response to different types of environmental stimuli in Schizosaccharomyces pombe. Upon stress activation, Sty1 reversibly accumulates in the nucleus, where it stimulates gene expression via the Atf1 transcription factor. The Atf1 protein forms a heterodimer with Pcr1, but the specific role of this association is controversial. We have carried out a comparative analysis of strains lacking these proteins individually. We demonstrate that Atf1 and Pcr1 have similar but not identical roles in S. pombe, since cells lacking Pcr1 do not share all the phenotypes reported for ⌬atf1 cells. Northern blot and microarray analyses demonstrate that the responses to specific stresses of cells lacking either Pcr1 or Atf1 do not fully overlap, and even though most Atf1-dependent genes induced by osmotic stress are also Pcr1 dependent, a subset of genes require only the presence of Atf1 for their induction. Whereas binding of Atf1 to most stress-dependent genes requires the presence of Pcr1, we demonstrate here that Atf1 can bind to the Pcr1-independent promoters in a ⌬pcr1 strain in vivo. Furthermore, these analyses show that both proteins have a global repressive effect on stress-dependent and stress-independent genes.

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confidence: 51%

Transcription Factors Pcr1 and Atf1 Have Distinct Roles in Stress- and Sty1-Dependent Gene Regulation

et al. 2008

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“…We previously reported that the Atf1-Pcr1-M26 complex links stress-activated mitogen-activated protein kinase and protein kinase A signaling pathways via chromatin remodeling in the cgs2 ϩ promoter (3). In that case, we suggested that the chromatin remodeling facilitates transcriptional activation of cgs2 ϩ (3).…”

Section: Vol 6 2007 Chromatin Configuration At Meiotic Dsb Sites Inmentioning

confidence: 86%

Multiple Modes of Chromatin Configuration at Natural Meiotic Recombination Hot Spots in Fission Yeast

et al. 2007

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The ade6-M26 meiotic recombination hot spot of fission yeast is defined by a cyclic AMP-responsive element (CRE)-like heptanucleotide sequence, 5-ATGACGT-3, which acts as a binding site for the Atf1/Pcr1 heterodimeric transcription factor required for hot spot activation. We previously demonstrated that the local chromatin around the M26 sequence motif alters to exhibit higher sensitivity to micrococcal nuclease before the initiation of meiotic recombination. In this study, we have examined whether or not such alterations in chromatin occur at natural meiotic DNA double-strand break (DSB) sites in Schizosaccharomyces pombe. At one of the most prominent DSB sites, mbs1 (meiotic break site 1), the chromatin structure has a constitutively accessible configuration at or near the DSB sites. The establishment of the open chromatin state and DSB formation are independent of the CRE-binding transcription factor, Atf1. Analysis of the chromatin configuration at CRE-dependent DSB sites revealed both differences from and similarities to mbs1. Eukaryotic chromosomal DNA is compacted with histones and other proteins to form chromatin, which helps in efficient storage of genetic information. However, this prevents many DNA-associated processes, such as transcription, replication, repair, and recombination, from accessing the DNA template (38). Homologous recombination contributes to gaining genetic diversity in the next generation as well as proper segregation of homologous chromosomes in meiosis. Meiotic recombination is a highly regulated process, with some loci that are elevated (hot spots) or suppressed (cold spots) (15,18,22,34).In the distantly related budding and fission yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, meiotic recombination hot spots are closely associated with sites of DNA double-strand breaks (DSBs), which are introduced by Spo11 (or its ortholog in S. pombe, Rec12) and are required for initiation of recombination (7,22,28,30). Elevated sensitivity of chromatin to micrococcal nuclease (MNase) is found at meiosis-specific hot spots in both budding and fission yeasts (17,21,39).The ade6-M26 hot spot of fission yeast is the only reported eukaryotic hot spot whose essential nucleotide sequence, 5Ј-A TGACGT-3Ј, has been precisely defined. The M26 hot spot confers a meiosis-specific elevation of recombination of up to 20-fold compared with other ade6 alleles (e.g., ade6-M375) (8,23,25). The ade6-M26 allele is a single G/T transversion at the 5Ј end of the ade6 coding region (23,31). This mutation creates a nonsense codon and cyclic AMP-responsive element (CRE)-like heptanucleotide sequence. The heptamer acts as a binding site for the Atf1/Pcr1 (also called Mts1/Mts2 or Gad7/Pcr1) heterodimeric transcription factor, which is required for hot spot activation (14,35). We have demonstrated that local chromatin with the M26 sequence motif becomes more sensitive to MNase in the early stage of meiosis, suggesting active chromatin remodeling around M26 (17). Furthermore, we have shown that Atf1 facil...

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“…A similar mechanism might operate in the control of ste11 expression in response to nitrogen starvation. Furthermore, it is noteworthy that the Sty1 pathway regulates transcription of some genes that are relevant for the cAMP-PKA pathway (Stiefel et al, 2004;Davidson et al, 2004), implicating that there is a complex crosstalk between these pathways.…”

Section: Perspectivesmentioning

confidence: 99%

Signaling pathways for fission yeast sexual differentiation at a glance

Otsubo

Yamamoto

2012

Journal of Cell Science

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Atf1-Pcr1-M26 Complex Links Stress-activated MAPK and cAMP-dependent Protein Kinase Pathways via Chromatin Remodeling of cgs2+

Cited by 45 publications

References 49 publications

Transcription Factors Pcr1 and Atf1 Have Distinct Roles in Stress- and Sty1-Dependent Gene Regulation

Transcription Factors Pcr1 and Atf1 Have Distinct Roles in Stress- and Sty1-Dependent Gene Regulation

Multiple Modes of Chromatin Configuration at Natural Meiotic Recombination Hot Spots in Fission Yeast

Signaling pathways for fission yeast sexual differentiation at a glance

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