Atherosclerosis prevention by nutritional factors: A meta-analysis in small animal models

Jové, Mariona; Pamplona, Reinald; Prat, Joan; Arola, Lluı́s; Portero-Otı́n, Manel

doi:10.1016/j.numecd.2012.09.006

Cited by 11 publications

(4 citation statements)

References 28 publications

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“…With respect to lipid and glucose metabolism, hamsters, like humans, exhibit high levels of cholesteryl ester transport protein (CETP), intestinal-only ApoB editing, low levels of hepatic low-density lipoprotein (LDL) receptor activity 6 and a high glycemic response to dietary fructose 7 , all of which are not observed in other rodents such as mice and rats. Consequently, hamsters, like humans, exhibit enhanced susceptibility to atherosclerosis (AS) and diabetes 8 , which led to the widespread use of hamsters in studies on AS and diabetes.…”

Section: Dear Editormentioning

confidence: 99%

Generation of transgenic golden Syrian hamsters

et al. 2014

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Golden Syrian hamsters are small rodents, but they display many features that resemble the physiology and metabolism of humans. [7], all of which are not observed in other rodents such as mice and rats. Consequently, hamsters, like humans, exhibit enhanced susceptibility to atherosclerosis (AS) and diabetes [8], which led to the widespread use of hamsters in studies on AS and diabetes.In the past 2-3 decades, due to the fast development of transgenic and knockout mice, hamsters were gradually replaced by these mouse models. However, due to multiple differences between mice and humans with respect to physiology and metabolism, the use of genemanipulated mice has limited value in disease modeling and pathophysiological studies. Extensive literature search has revealed an absence of reports on genetically manipulated hamster models. To capitalize on the special metabolic features of hamsters, we aim to generate genemanipulated hamsters as an alternate rodent model for general applications. As the initial step to create a genetically manipulated hamster, we utilized a highly efficient lentiviral vector to generate transgenic hamsters expressing enhanced green fluorescent protein (eGFP).By modifying and optimizing the protocols for producing transgenic mice and rabbits in our laboratory [9, 10], we developed a specific procedure for hamster superovulation, fertilized egg harvesting, perivitelline space microinjection and embryo transfer. After the successful culture of fertilized hamster eggs that developed into 4-and 8-cell embryos in vitro ( Figure 1A), we implanted these embryos into pseudopregnant females. We obtained 7-10 pups/litter in 4 out of 7 surrogate mothers. Next, we microinjected 50-100 picoliters of a lentiviral

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Section: Dear Editormentioning

confidence: 99%

Generation of transgenic golden Syrian hamsters

et al. 2014

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“…Increased obesity is observed in rodents when placed on a higher-fat diet. However, due to physiological differences, specific cardiometabolic markers (such as low HDL cholesterol, increased LDL cholesterol, and coronary calcification) translate better in pigs and primates than in rodent models [10,11]. In fact, lipoprotein metabolism in primates and swine is very similar to that of humans.…”

Section: Introductionmentioning

confidence: 99%

High-Fat Diet-Induced Obesity Increases Brain Mitochondrial Complex I and Lipoxidation-Derived Protein Damage

Berdún,

Obis,

Mota-Martorell

et al. 2024

Antioxidants

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Obesity is a risk factor for highly prevalent age-related neurodegenerative diseases, the pathogenesis of whichinvolves mitochondrial dysfunction and protein oxidative damage. Lipoxidation, driven by high levels of peroxidizable unsaturated fatty acids and low antioxidant protection of the brain, stands out as a significant risk factor. To gain information on the relationship between obesity and brain molecular damage, in a porcine model of obesity we evaluated (1) the level of mitochondrial respiratory chain complexes, as the main source of free radical generation, by Western blot; (2) the fatty acid profile by gas chromatography; and (3) the oxidative modification of proteins by mass spectrometry. The results demonstrate a selectively higher amount of the lipoxidation-derived biomarker malondialdehyde-lysine (MDAL) (34% increase) in the frontal cortex, and positive correlations between MDAL and LDL levels and body weight. No changes were observed in brain fatty acid profile by the high-fat diet, and the increased lipid peroxidative modification was associated with increased levels of mitochondrial complex I (NDUFS3 and NDUFA9 subunits) and complex II (flavoprotein). Interestingly, introducing n3 fatty acids and a probiotic in the high-fat diet prevented the observed changes, suggesting that dietary components can modulate protein oxidative modification at the cerebral level and opening new possibilities in neurodegenerative diseases’ prevention.

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“…Animal models are essential to understand the physiological mechanisms of ACVD in humans. However, lipoprotein metabolism and vascular physiology are often contrasted between species limiting the transposition of findings from animals to human 4 , 5 . Such differences should be considered for animal model selection and for data understanding within a realistic extrapolation framework for a better assessment of disturbances involved in humans 6 .…”

Section: Introductionmentioning

confidence: 99%

Plasma lipidomic analysis reveals strong similarities between lipid fingerprints in human, hamster and mouse compared to other animal species

Kaabia

Poirier

Moughaizel

et al. 2018

Sci Rep

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Cardiovascular diseases are often associated with impaired lipid metabolism. Animal models are useful for deciphering the physiological mechanisms underlying these pathologies. However, lipid metabolism is contrasted between species limiting the transposition of findings from animals to human. Hence, we aimed to compare extended lipid profiles of several animal species to bring new insights in animal model selections. Human lipid phenotype was compared with those of 10 animal species. Standard plasma lipids and lipoprotein profiles were obtained by usual methods and lipidomic analysis was conducted by liquid chromatography-high-resolution mass spectrometry (LC-HRMS). As anticipated, we found contrasted lipid profiles between species. Some of them exhibited similar plasma lipids to human (non-human primate, rat, hamster, pig), but only usual lipid profiles of pigs were superimposable with human. LC-HRMS analyses allowed the identification of 106 other molecular species of lipids, common to all samples and belonging to major lipid families. Multivariate analyses clearly showed that hamster and, in a lower extent mouse, exhibited close lipid fingerprints to that of human. Besides, several lipid candidates that were previously reported to study cardiovascular diseases ranged similarly in human and hamster. Hence, hamster appeared to be the best option to study physiological disturbances related to cardiovascular diseases.

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Atherosclerosis prevention by nutritional factors: A meta-analysis in small animal models

Cited by 11 publications

References 28 publications

Generation of transgenic golden Syrian hamsters

Generation of transgenic golden Syrian hamsters

High-Fat Diet-Induced Obesity Increases Brain Mitochondrial Complex I and Lipoxidation-Derived Protein Damage

Plasma lipidomic analysis reveals strong similarities between lipid fingerprints in human, hamster and mouse compared to other animal species

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