AtHKT1 gene regulating K+ state in whole plant improves salt tolerance in transgenic tobacco plants

Wang, Li; Liu, Yuhui; Feng, Shoujiang; Wang, Zhuoyu; Jin-wen, Zhang; Zhang, Junlian; Wang, Di; Gan, Yantai

doi:10.1038/s41598-018-34660-9

Cited by 20 publications

(10 citation statements)

References 46 publications

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“…Maintaining potassium balance is an essential part of plant response to salt stress. AtHKT1 regulates K + state ( Wang et al, 2018 ), whereas HvHKT1 ; 5 in stele cells, negatively regulates salt tolerance in barley ( Huang et al, 2020 ). Overexpression of OsHAK5 increases the K + /Na + ratio and tolerance to salt stress in rice seedlings ( Yang, Zhang & Hu, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and structure analysis in root of Casuarina equisetifolia under NaCl treatment

Wang

Zhang

Zhenfei

et al. 2021

PeerJ

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Background High soil salinity seriously affects plant growth and development. Excessive salt ions mainly cause damage by inducing osmotic stress, ion toxicity, and oxidation stress. Casuarina equisetifolia is a highly salt-tolerant plant, commonly grown as wind belts in coastal areas with sandy soils. However, little is known about its physiology and the molecular mechanism of its response to salt stress. Results Eight-week-old C. equisetifolia seedlings grown from rooted cuttings were exposed to salt stress for varying durations (0, 1, 6, 24, and 168 h under 200 mM NaCl) and their ion contents, cellular structure, and transcriptomes were analyzed. Potassium concentration decreased slowly between 1 h and 24 h after initiation of salt treatment, while the content of potassium was significantly lower after 168 h of salt treatment. Root epidermal cells were shed and a more compact layer of cells formed as the treatment duration increased. Salt stress led to deformation of cells and damage to mitochondria in the epidermis and endodermis, whereas stele cells suffered less damage. Transcriptome analysis identified 10,378 differentially expressed genes (DEGs), with more genes showing differential expression after 24 h and 168 h of exposure than after shorter durations of exposure to salinity. Signal transduction and ion transport genes such as HKT and CHX were enriched among DEGs in the early stages (1 h or 6 h) of salt stress, while expression of genes involved in programmed cell death was significantly upregulated at 168 h, corresponding to changes in ion contents and cell structure of roots. Oxidative stress and detoxification genes were also expressed differentially and were enriched among DEGs at different stages. Conclusions These results not only elucidate the mechanism and the molecular pathway governing salt tolerance, but also serve as a basis for identifying gene function related to salt stress in C. equisetifolia.

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Section: Discussionmentioning

confidence: 99%

Transcriptome and structure analysis in root of Casuarina equisetifolia under NaCl treatment

Wang

Zhang

Zhenfei

et al. 2021

PeerJ

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“…The HKT genes also play a crucial role in Na + homeostasis by retrieving Na + from the xylem sap, thereby limiting its accumulation in leaves (Zhu et al 2017). Ectopic expression of AtHKT1 has been revealed to enhance salinity tolerance in Nicotiana tabacum (Wang et al 2018). Likewise, ZmHKT1;5 has also been ectopically expressed in Nicotiana tabacum (Jiang et al 2018).…”

Section: Engineering Crop Plants For Ion Homeostasis‐mediated Salt Tomentioning

confidence: 99%

Reassessing the role of ion homeostasis for improving salinity tolerance in crop plants

Basu

Kumar

Benazir

et al. 2020

Physiologia Plantarum

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Soil salinity is a constraint for major agricultural crops leading to severe yield loss, which may increase with the changing climatic conditions. Disruption in the cellular ionic homeostasis is one of the primary responses induced by elevated sodium ions (Na+). Therefore, unraveling the mechanism of Na+ uptake and transport in plants along with the characterization of the candidate genes facilitating ion homeostasis is obligatory for enhancing salinity tolerance in crops. This review summarizes the current advances in understanding the ion homeostasis mechanism in crop plants, emphasizing the role of transporters involved in the regulation of cytosolic Na+ level along with the conservation of K+/Na+ ratio. Furthermore, expression profiles of the candidate genes for ion homeostasis were also explored under various developmental stages and tissues of Oryza sativa based on the publicly available microarray data. The review also gives an up‐to‐date summary on the efforts to increase salinity tolerance in crops by manipulating selected stress‐associated genes. Overall, this review gives a combined view on both the ionomic and molecular background of salt stress tolerance in plants.

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“…TaHKT1 from wheat, a member of subclass-2, at low concentrations of Na + works as Na + /K + symporter, but at high concentration of Na + TaHKT1 act as a Na + uniporter [ 8 ]. It has been recently demonstrated, via transgenic analysis, that over-expression of AtHKT1 contributes to maintain optimal K + /Na + in tobacco plants and to improve plant biomass under salt stress [ 55 ]. However, different modifications of HKT1 transporters may also cause variations of leaf Na + exclusion and salt tolerance in maize .…”

Section: Role Of Hkt1-type Transporters In Glycophytes and Halophymentioning

confidence: 99%

Role and Functional Differences of HKT1-Type Transporters in Plants under Salt Stress

Ali

Maggio

Bressan

et al. 2019

IJMS

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Abiotic stresses generally cause a series of morphological, biochemical and molecular changes that unfavorably affect plant growth and productivity. Among these stresses, soil salinity is a major threat that can seriously impair crop yield. To cope with the effects of high salinity on plants, it is important to understand the mechanisms that plants use to deal with it, including those activated in response to disturbed Na+ and K+ homeostasis at cellular and molecular levels. HKT1-type transporters are key determinants of Na+ and K+ homeostasis under salt stress and they contribute to reduce Na+-specific toxicity in plants. In this review, we provide a brief overview of the function of HKT1-type transporters and their importance in different plant species under salt stress. Comparison between HKT1 homologs in different plant species will shed light on different approaches plants may use to cope with salinity.

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AtHKT1 gene regulating K+ state in whole plant improves salt tolerance in transgenic tobacco plants

Cited by 20 publications

References 46 publications

Transcriptome and structure analysis in root of Casuarina equisetifolia under NaCl treatment

Transcriptome and structure analysis in root of Casuarina equisetifolia under NaCl treatment

Reassessing the role of ion homeostasis for improving salinity tolerance in crop plants

Role and Functional Differences of HKT1-Type Transporters in Plants under Salt Stress

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