Atividade Citotóxica Do Extrato De Croton Urucurana Baill Contra Linhagens De Células Leucêmicas Humanas U937 E Thp1

Vieira, Geisla Teles; Oliveira, Tânia Toledo de; Monteiro, Luana Pereira; Kanashiro, Milton; Costa, Marcelo Rocha da; Pereira, Willian

doi:10.5902/2179460x23281

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…Other researchers have shown a dose-dependent effect over time, as proposed by Vieira et al [10], which demonstrated apoptosis in cell line U937 and THP-1 by the extract of Croton urucurana in a single application dose. They showed that at concentrations of 400 and 800 µg/mL, and over a period of 48 hours, the extract promotes 75% of apoptosis, demonstrating the desired effect, over a long period, from the ethanol extract.…”

Section: Discussionmentioning

confidence: 79%

In vitro methodological standardization of MTT colorimetric assay for evaluation of formazan activity and drug dosage

Nepomuceno

Carvalho

Soares

et al. 2020

Preprint

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Background Aiming to develop essential chemotherapeutic drugs to control various types of cancer, one of the primary tests is the tetrazolium reduction method. The present study aims to standardize the electromagnetic spectrum that best discriminates the absorbance and to evaluate the drug dosage of single and daily doses during the cell viability and toxicity analysis by the tetrazolium reduction method, using the ethanol extract of pequi peel in canine osteosarcoma cells. Results The wavelength of 532 nm showed the best results. We found that as the treatment time increased, the formazan conversion reduced. After 72 hours of treatment, we observed clear discrimination of dose-dependent data, with up to five discriminants within 72 hours with a change in absorbance from 0.554 to 0.064 A. The wavelength of 570 nm it was not ideal since we could not discern the difference in the spectral reflectance of the treatments and, therefore, show no statistical difference among treatments. We found no statistical difference for the coefficient of variation at wavelengths of 532 and 570nm, which were 12.77% and 8.80% respectively. Conclusions The wavelength of 532nm best discriminated the absorbance, as it presented better ability to congregate the treatment groups, greater variation between the discriminant and lower coefficient of variation, during the colorimetric test to evaluate the cellular metabolism. Moreover, the ethanol extract of pequi peel in canine osteosarcoma cells showed a statistically equal effect of a single dose administration to the dose reapplied every 24 hours.

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Section: Discussionmentioning

confidence: 79%

In vitro methodological standardization of MTT colorimetric assay for evaluation of formazan activity and drug dosage

Nepomuceno

Carvalho

Soares

et al. 2020

Preprint

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“…Outros estudos envolveram a investigação de propriedades antibacterianas e antioxidantes do óleo essencial das folhas (SIMIONATTO et al, 2007) e atividade citotóxica do extrato da casca contra linhagens de células leucêmicas humanas (VIEIRA et al,2017).…”

Section: Introductionunclassified

Linhagens Mutantes WTS De Drosophila Melanogastercomo Modelode Avaliação De Atividade Anticarcinogênica Do Látex Decroton Urucurana

Costa¹,

Soares²,

Gouveia³

et al. 2023

Biologia: Contextualizando O Conhecimento Científico

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Objetivo: O látex de Croton urucurana é largamente utilizado pela medicina popular no tratamento de câncer, diarreia, reumatismo, como cicatrizante de feridas e úlceras. Este trabalho teve por objetivo avaliar a atividade anticarcinogênica do látex de Croton urucurana. Métodos: Para realização do teste wts (warts) preparou-se soluções do látex da Croton urucurana, a 2,5%, 5% e 10% em água deionizada e utilizou-se duas linhagens mutantes de Drosophila melanogaster: wts e mwh, portadoras dos marcadores genéticos Warts (wts, 3-100) e multiple wing hairs, (3-03). Para controle positivo utilizou-se a DXR (0,4 mM) e para controle negativo água de osmose reversa. Para registrar a frequência de tumores, utilizou-se uma planilha padrão, com a incidência de tumores nas regiões do olho, cabeça, asa, corpo, perna, halteres e o total por mosca, em cada concentração testada. Resultados: Observou-se que há associação entre o controle positivo e maiores casos de tumores na perna (P≤ 0,001; X2= 47), asa (P≤ 0,001; X2 = 133,28 e corpo (P = 0,003; X2 = 13,61). A análise do risco relativo revelou aumento de tumores em D. melanogaster, sendo 2% na concentração de 2,5% (>01); 2,3% (>01) na fração de 5% e 3,5% (>01) na concentração de 10%. Conclusão: Há evidências de que o látex de C. urucurana não possui atividade anticarcinogênica e que este composto, sob essas condições experimentais, apresenta efeito carcinogênico em D. melanogaster.

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“…Popularly known as sangra d´água, it is used as antiinflammatory, anti-hemorrhagic, healing, in the fight against prostate cancer, diabetes and kidney disease (ANTONIAZZI et al, 2016). Some studies report the chemical composition and biological actions for latex, essential oil and different parts of the plant, highlighting cytotoxic, antimicrobial and antinociceptive activities (CANDIDO-BACANI et al, 2015;SIMIONATTO et al, 2007;SIMIONATTO et al, 2009;VIEIRA et al, 2017;CORDEIRO et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

ANTIOXIDANT ACTIVITY AND TOTAL PHENOL, FLAVONOID AND TANNIN CONTENTS OF Croton floribundus AND Croton urucurana

Silva¹,

Feitosa²,

Lima³

et al. 2019

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Atividade Citotóxica Do Extrato De Croton Urucurana Baill Contra Linhagens De Células Leucêmicas Humanas U937 E Thp1

Abstract: RESUMO As leucemias são neoplasias malignas que acometem as células do sangue e têm origem na medula óssea. São cânceres de grande incidência e de difícil tratamento, havendo, portanto, a uma constante procura por terapias

Cited by 7 publications

References 21 publications

In vitro methodological standardization of MTT colorimetric assay for evaluation of formazan activity and drug dosage

In vitro methodological standardization of MTT colorimetric assay for evaluation of formazan activity and drug dosage

Linhagens Mutantes WTS De Drosophila Melanogastercomo Modelode Avaliação De Atividade Anticarcinogênica Do Látex Decroton Urucurana

ANTIOXIDANT ACTIVITY AND TOTAL PHENOL, FLAVONOID AND TANNIN CONTENTS OF Croton floribundus AND Croton urucurana

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