Atlantic salmon (Salmo salar) co-product-derived protein hydrolysates: A source of antidiabetic peptides

Harnedy, Pádraigín A.; Parthsarathy, Vadivel; McLaughlin, Chris M.; O’Keeffe, Martina B.; Allsopp, Philip J.; McSorley, Emeir M.; O’Harte, Finbarr; Fitzgerald, Richard J.

doi:10.1016/j.foodres.2018.01.025

Cited by 95 publications

(68 citation statements)

References 56 publications

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“…Thus, salmon frames were rich in protein, mainly from the remaining meat and minerals were mostly from bones. This was in agreement with Harnedy et al () who documented that salmon skin and trimmings derived during salmon filleting process, contained significant quantities of protein. Salmon frame showed higher ash content than Alaska pollock counterpart (14.99%) (Hou, Li, Zhao, Zhang, & Li, ).…”

Section: Resultssupporting

confidence: 92%

Protein hydrolysate from salmon frames: Production, characteristics and antioxidative activity

et al. 2018

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Protein hydrolysates from two forms of salmon frames named “chunk” and “mince” were produced and characterized. Both samples were subjected to hydrolysis using alcalase and papain at 1%–3% (w/w protein) for 0–240 min. Hydrolysate prepared with either protease at 3% for 180 min had the solid yield of 24.05%–26.39%. Hydrolysates contained 79.20%–82.01% proteins, 6.03%–6.34% fat, 9.81%–11.09% ash, and 4.02%–5.80% moisture. Amino acid profile showed that all hydrolysates had glutamic acid/glutamine (113.45–117.56 mg/g sample), glycine (77.86–86.18 mg/g sample), aspartic acid/asparagine (76.04–78.67 mg/g sample), lysine (61.97–65.99 mg/g sample), and leucine (54.30–57.31 mg/g sample) as the predominant amino acids. The size distributions determined by gel filtration chromatography varied, depending on proteases and the form of frame used for the hydrolysis. Different hydrolysates showed varying antioxidant capacities. Thus, protein hydrolysates from salmon frame could be used as a nutritive supplement in the protein deficient foods. Practical applications Frames of salmon are by‐products from salmon fish processing industries. The frames contained the remaining meat, hence they can be used for the preparation of protein hydrolysates. Generally, hydrolysates from fish by‐products have been regarded as a promising food supplement, because they are rich in amino acids. Additionally, hydrolysates possess antioxidant activity, which is of health benefit. To produce the hydrolysate with less time consumption, the use of frame chunk instead of minced frame can be of better choice. Thus, frame of salmon, especially in chunk form, could be used as a raw material for production of protein hydrolysate using alcalase. The hydrolysate produced from salmon frame could serve as an alternative nutritive supplement to tackle the nutrition inadequacies in foods.

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Section: Resultssupporting

confidence: 92%

Protein hydrolysate from salmon frames: Production, characteristics and antioxidative activity

et al. 2018

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“…This was in line with their pronounced insulinotropic actions in vitro . The present findings compare favourably with results published recently with other fish hydrolysates (Harnedy et al ., ,b). For example, in the case of blue whiting ( Micromesistius poutassou ) a two‐fold higher dose of protein hydrolysate (100 mg mL −1 ) generated using Alcalase 2.4 L and Flavourzyme 500 L had a similar acute glucose‐lowering effect (22% AUC (0–120 min) reduction) following an OGTT in normal healthy mice (Harnedy et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…Research into marine protein and its subsequent hydrolysates and isolated bioactive peptides targeting diabetes and its complications is limited. Nevertheless, some early studies have shown the beneficial effects of inhibiting dipeptidyl peptidase‐4 (DPP‐IV) (Huang et al ., ; Harnedy et al ., , ,b; Silaa et al ., ), and thus lowering glycated haemoglobin (HbA 1c ) in human volunteers with T2DM (Zhu et al ., ). The aims of the present study were firstly to investigate the in vitro insulinotropic and GLP‐1 stimulatory effects of a BPH, and secondly to investigate the effect of oral consumption of BPH on blood glucose control in healthy mice following an oral glucose challenge.…”

Section: Introductionmentioning

confidence: 99%

Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP‐1 secretory activity in vitro and acute glucose lowering effects in mice

Parthsarathy

McLaughlin

Harnedy

et al. 2018

Int J of Food Sci Tech

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The anti-diabetic actions of a boarfish protein hydrolysate (BPH) were investigated in cultured cells and mice. A boarfish (Capros aper) muscle protein hydrolysate was generated using the enzymes Alcalase 2.4 L and Flavourzyme 500 L. Furthermore, the BPH was subjected to simulated gastrointestinal digestion (SGID). BPH and SGID samples (0.01-2.5 mg mL À1 ) were tested in vitro for DPP-IV inhibition and insulin and GLP-1 secretory activity from BRIN-BD11 and GLUTag cells, respectively. The BPH and SGID samples, caused a dose-dependent increase (4.2 to 5.3-fold, P < 0.001) in insulin secretion from BRIN-BD11 cells and inhibited DPP-IV activity (IC 50 1.18 AE 0.04 and 1.21 AE 0.04 mg mL À1 ), respectively. The SGID sample produced a 1.3-fold (P < 0.01) increase in GLP-1 secretion. An oral glucose tolerance test (OGTT) was conducted in healthy mice (n = 8), with or without BPH (50 mg/kg bodyweight). BPH mediated an increase in plasma insulin levels (AUC (0-120 min), P < 0.05) and a consequent reduction in blood glucose concentration (P < 0.01), after OGTT in mice versus controls. The BPH showed potent anti-diabetic actions in cells and improved glucose tolerance in mice.Keywords Boarfish protein hydrolysate, dipeptidyl peptidase-IV inhibition, glucagon-like peptide-1, glycaemic control in mice, insulin secretion.

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“…In Table 4, we have summarized examples of bioactive compounds recovered from animal and marine products processing wastes. [170][171][172]…”

Section: Bioactive Compounds From Animal Products Processing Wastementioning

confidence: 99%

“…Salmon nasal cartilage is a valuable source of bioactive proteoglycans reported for anti-angiogenic activity, relieving joint pain discomfort in elderly people and promoting wound healing [167][168][169]177]. Fish skin is also an important source of collagen that can be hydrolysed to bioactive peptides with multiple health benefits with antioxidant, anti-hypertensive, and anti-diabetic activities, as well as enhancing learning and memory [170][171][172].…”

Section: Fish and Shellfish Wastementioning

confidence: 99%

Bioactives from Agri-Food Wastes: Present Insights and Future Challenges

2020

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Sustainable utilization of agri-food wastes and by-products for producing value-added products (for cosmetic, pharmaceutical or food industrial applications) provides an opportunity for earning additional income for the dependent industrial sector. Besides, effective valorisation of wastes/by-products can efficiently help in reducing environmental stress by decreasing unwarranted pollution. The major focus of this review is to provide comprehensive information on valorisation of agri-food wastes and by-products with focus laid on bioactive compounds and bioactivity. The review covers the bioactives identified from wastes and by-products of plants (fruits, exotic fruits, vegetables and seeds), animals (dairy and meat) and marine (fish, shellfish seaweeds) resources. Further, insights on the present status and future challenges of sustainably utilizing agri-food wastes/by-products for value addition will be highlighted.

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Atlantic salmon (Salmo salar) co-product-derived protein hydrolysates: A source of antidiabetic peptides

Cited by 95 publications

References 56 publications

Protein hydrolysate from salmon frames: Production, characteristics and antioxidative activity

Protein hydrolysate from salmon frames: Production, characteristics and antioxidative activity

Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP‐1 secretory activity in vitro and acute glucose lowering effects in mice

Bioactives from Agri-Food Wastes: Present Insights and Future Challenges

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