Atlas of Gene Expression in the Developing Kidney at Microanatomic Resolution

Brunskill, Eric W.; Aronow, Bruce J.; Georgas, Kylie; Rumballe, Bree; Valerius, M. Todd; Aronow, Jeremy; Kaimal, Vivek; Jegga, Anil G.; Grimmond, Sean M.; McMahon, Andrew P.; Patterson, Larry T.; Little, Melissa H.; Potter, S. Steven

doi:10.1016/j.devcel.2008.09.007

Cited by 198 publications

(195 citation statements)

References 34 publications

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“…The ureteric buds grow and branch to form tubules with polarized epithelial cells. By E15.5, most of the kidney components appear as immature anlages, which include the S-shaped body, loop of Henle, and collecting ducts (Stuart and Nigam, 1995;Brunskill et al, 2008;Georgas et al, 2008). The expression of Arf6 begins earlier than E13.5.…”

Section: Expression In the Kidneymentioning

confidence: 99%

Tissue‐ and development‐dependent expression of the small GTPase Arf6 in mice

Akiyama

Zhou

Sugimoto

et al. 2010

Developmental Dynamics

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The small GTPase Arf6 is a member of the Arf (ADP-ribosylation factor) family. Although the function of Arf6 has been heavily studied at the cellular level, its physiological function at the whole animal level is largely unknown. In this study, we examined both the tissue distribution and developmental timing of Arf6 expression in wild type mice to obtain valuable information to speculate on the physiological function of Arf6. Western blot analysis using anti-Arf6 antibody revealed that Arf6 was ubiquitously expressed with its developmental timing differing in a tissue-specific manner. These results were supported by Arf6 mRNA in situ hybridization experiments, which showed that Arf6 was highly expressed in the polarized epithelial cells and embryonic mesenchymal cells of most tissues in a temporally dependent manner. Taken in toto, our results suggest that the expression of Arf6 in mouse tissues is precisely regulated in a developmentand tissue-dependent manner. Developmental Dynamics 239:3416-3435,

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Section: Expression In the Kidneymentioning

confidence: 99%

Tissue‐ and development‐dependent expression of the small GTPase Arf6 in mice

Akiyama

Zhou

Sugimoto

et al. 2010

Developmental Dynamics

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“…As an initial approach to the identification of marker genes, an atlas of gene expression during kidney development was generated based upon microarray gene expression analysis. 19 Using laser capture microdissection, 15 distinct anatomical compartments of the developing kidney were isolated, including a number of distinct portions of the developing nephron. Affymetrix gene expression profiling was then performed for each anatomical compartment.…”

Section: Renal Organogenesismentioning

confidence: 99%

“…Section in situ hybridization also supported the quality of this analysis. 19 While many genes show relative differences in the level of expression in different cellular compartments, we proposed that the most valuable gene sets that we could identify from this data set were those where genes showed absolute compartment specificity. We defined such genes as "anchor genes" and selected potential anchor genes based on bioinformatics (Fig.…”

Section: Renal Organogenesismentioning

confidence: 99%

Renal organogenesis

Little

2011

Organogenesis

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“…Recent microanatomic gene expression studies have revealed an unanticipated complexity of cell types within each of these cellular compartments (Brunskill et al, 2008), and complementary methods such as NZC culture are required to understand the interrelationship between these cells during nephrogenesis. The ultimate goal of our primary cell investigations is to define conditions in which nephrogenic zone cells can be indefinitely expanded in vitro both for studies of nephrogenesis and engraftment in the adult.…”

mentioning

confidence: 99%

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

Brown

Blank

Adams

et al. 2011

JoVE

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Embryonic development of the kidney has been extensively studied both as a model for epithelial-mesenchymal interaction in organogenesis and to gain understanding of the origins of congenital kidney disease. More recently, the possibility of steering naïve embryonic stem cells toward nephrogenic fates has been explored in the emerging field of regenerative medicine. Genetic studies in the mouse have identified several pathways required for kidney development, and a global catalog of gene transcription in the organ has recently been generated http://www.gudmap.org/, providing numerous candidate regulators of essential developmental functions. Organogenesis of the rodent kidney can be studied in organ culture, and many reports have used this approach to analyze outcomes of either applying candidate proteins or knocking down the expression of candidate genes using siRNA or morpholinos. However, the applicability of organ culture to the study of signaling that regulates stem/progenitor cell differentiation versus renewal in the developing kidney is limited as cultured organs contain a compact extracellular matrix limiting diffusion of macromolecules and virus particles. To study the cell signaling events that influence the stem/progenitor cell niche in the kidney we have developed a primary cell system that establishes the nephrogenic zone or progenitor cell niche of the developing kidney ex vivo in isolation from the epithelial inducer of differentiation. Using limited enzymatic digestion, nephrogenic zone cells can be selectively liberated from developing kidneys at E17.5. Following filtration, these cells can be cultured as an irregular monolayer using optimized conditions. Marker gene analysis demonstrates that these cultures contain a distribution of cell types characteristic of the nephrogenic zone in vivo, and that they maintain appropriate marker gene expression during the culture period. These cells are highly accessible to small molecule and recombinant protein treatment, and importantly also to viral transduction, which greatly facilitates the study of candidate stem/progenitor cell regulator effects. Basic cell biological parameters such as proliferation and cell death as well as changes in expression of molecular markers characteristic of nephron stem/progenitor cells in vivo can be successfully used as experimental outcomes. Ongoing work in our laboratory using this novel primary cell technique aims to uncover basic mechanisms governing the regulation of self-renewal versus differentiation in nephron stem/progenitor cells. Video LinkThe video component of this article can be found at http://www.jove.com/details.php?id=2555 (96, 48, 24 or 6 well) with human fibronectin solution at a concentration of 5 mg per cm 2 of growth surface. 5 mg of fibronectin powder is resuspended in 5 ml of sterile H2O. This is diluted 1:25 in sterile DPBS without calcium or magnesium and 250 ml is added to each well of a 24 well plate. Plates are then incubated with fibronectin solution at room temperature for 1 hou...

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Atlas of Gene Expression in the Developing Kidney at Microanatomic Resolution

Cited by 198 publications

References 34 publications

Tissue‐ and development‐dependent expression of the small GTPase Arf6 in mice

Tissue‐ and development‐dependent expression of the small GTPase Arf6 in mice

Renal organogenesis

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

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