Atlas of rat fetal skeleton double stained for bone and cartilage

Menegola, Elena; Broccia, Maria Luisa; Giavini, Erminio

doi:10.1002/tera.1055

Cited by 77 publications

(56 citation statements)

References 12 publications

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“…1). In the occipital region, the ossification centers of occipital condyles were visible and these results were in correlation with Menegola et al (2001). At the facial region of skull, the ossification centers of the premaxilla and maxilla were visible separately (Fig.…”

supporting

confidence: 58%

Identification of Primary Ossification Centers in the Skull of Buffalo Fetus by Modified Alizarin Red-S Method

Ersavadla¹,

Nalla²

2017

Int. J. Livest. Res.

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In this modified alizarin red-s method the fetuses were fixed in 10 per cent Neutral buffered formalin for 1 week. Reduced the potassium hydroxide digestion time to secure the muscular system and then stained with alizarin red-s. Excess stain was removed by fresh 4 per cent potassium hydroxide solution. Stained fetuses were cleared for 2 weeks by using two clearing agents. The primary ossification centers of the skull bones were clearly visible. The fetuses were preserved in glycerin.

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supporting

confidence: 58%

Identification of Primary Ossification Centers in the Skull of Buffalo Fetus by Modified Alizarin Red-S Method

Ersavadla¹,

Nalla²

2017

Int. J. Livest. Res.

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“…Half of the live fetuses from each litter were fixed in absolute ethanol, eviscerated, and then processed for skeletal staining with alizarin red S and Alcyan blue using for subsequent skeletal examination. 30 The other half was preserved in Bouin solution and examined for internal softtissue changes using a freehand razor sectioning technique 31 and Nishimura's method. 32 The observed fetal morphological alterations in this study were classified as developmental malformations or variations.…”

Section: Postmortem Examinationmentioning

confidence: 99%

Prenatal development toxicity study of zinc oxide nanoparticles in rats

An¹,

Hong²,

Park³

et al. 2014

IJN

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This study investigated the potential adverse effects of zinc oxide nanoparticles ([ZnO SM20(+) NPs] zinc oxide nanoparticles, positively charged, 20 nm) on pregnant dams and embryo–fetal development after maternal exposure over the period of gestational days 5–19 with Sprague-Dawley rats. ZnO SM20(+) NPs were administered to pregnant rats by gavage at 0, 100, 200, and 400 mg/kg/day. All dams were subjected to a cesarean section on gestational day 20, and all of the fetuses were examined for external, visceral, and skeletal alterations. Toxicity in the dams manifested as significantly decreased body weight after administration of 400 mg/kg/day NPs; reduced food consumption after administration of 200 and 400 mg/kg/day NPs; and decreased liver weight and increased adrenal glands weight after administration of 400 mg/kg/day NPs. However, no treatment-related difference in: number of corpora lutea; number of implantation sites; implantation rate (%); resorption; dead fetuses; litter size; fetal deaths and placental weights; and sex ratio were observed between the groups. On the other hand, significant decreases between treatment groups and controls were seen for fetal weights after administration of 400 mg/kg/day NPs. Morphological examinations of the fetuses demonstrated significant differences in incidences of abnormalities in the group administered 400mg/kg/day. Meanwhile, no significant difference was found in the Zn content of fetal tissue between the control and high-dose groups. These results showed that oral doses for the study with 15-days repeated of ZnO SM20(+) NPs were maternotoxic in the 200 mg/kg/day group, and embryotoxic in the 400 mg/kg/day group.

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“…Half of the live fetuses from each litter were fixed in absolute ethanol, eviscerated, and then processed for skeletal staining with Alizarin red S and Alcyan blue, and were used for subsequent skeletal examination. 29 The other half was preserved in Bouin's solution and examined for internal soft tissue changes, using a freehand razor sectioning technique 30 and Nishimura's method. 31 The fetal morphological alterations observed in this study were classified as developmental malformations or variations.…”

Section: Postmortem Examinationmentioning

confidence: 99%

Effect of zinc oxide nanoparticles on dams and embryo–fetal development in rats

Hong

Park

Kim

et al. 2014

IJN

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This study investigated the potential adverse effects of zinc oxide nanoparticles (ZnO SM20[−] NPs; negatively charged, 20 nm) on pregnant dams and embryo–fetal development after maternal exposure over the period of gestational days 5–19 with Sprague Dawley rats. ZnO SM20(−) NPs were administered to pregnant rats by gavage at 0 mg/kg/day, 100 mg/kg/day, 200 mg/kg/day, and 400 mg/kg/day. All dams were subjected to caesarean section on gestational day 20, and all the fetuses were examined for external, visceral, and skeletal alterations. Toxicity in the dams manifested as significantly decreased body weight at 400 mg/kg/day and decreased liver weight, and increased adrenal glands weight at 200 mg/kg/day and 400 mg/kg/day. However, no treatment-related difference in the number of corpora lutea, the number of implantation sites, the implantation rate (%), resorption, dead fetuses, litter size, fetal deaths, fetal and placental weights, and sex ratio were observed between the groups. Morphological examinations of the fetuses demonstrated no significant difference in the incidences of abnormalities between the groups. No significant difference was found in the Zn content of fetal tissue between the control and high-dose groups. These results showed that a 15-day repeated oral dose of ZnO SM20(−) was minimally maternotoxic at dose of 200 mg/kg/day and 400 mg/kg/day.

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Atlas of rat fetal skeleton double stained for bone and cartilage

Cited by 77 publications

References 12 publications

Identification of Primary Ossification Centers in the Skull of Buffalo Fetus by Modified Alizarin Red-S Method

Identification of Primary Ossification Centers in the Skull of Buffalo Fetus by Modified Alizarin Red-S Method

Prenatal development toxicity study of zinc oxide nanoparticles in rats

Effect of zinc oxide nanoparticles on dams and embryo–fetal development in rats

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Atlas of rat fetal skeleton double stained for bone and cartilage

Cited by 77 publications

References 12 publications

Identification of Primary Ossification Centers in the Skull of Buffalo Fetus by Modified Alizarin Red-S Method

Identification of Primary Ossification Centers in the Skull of Buffalo Fetus by Modified Alizarin Red-S Method

Prenatal development toxicity study of zinc oxide nanoparticles in rats

Effect of zinc oxide nanoparticles on dams and&nbsp;embryo&ndash;fetal development in rats

Contact Info

Product

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Effect of zinc oxide nanoparticles on dams and embryo–fetal development in rats