ATM and ATR homologes of Neurospora crassa are essential for normal cell growth and maintenance of chromosome integrity

Wakabayashi, Michiyoshi; Ishii, Chizu; Hatakeyama, Susumi; Inoue, Hirokazu; Tanaka, Shuuitsu

doi:10.1016/j.fgb.2010.05.010

Cited by 12 publications

(19 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1A). As controls, we included mutagen-sensitive-9 (mus-9), lacking the Neurospora ATM homolog required for the DNA damage response (48), and mei-3, lacking the Neurospora homolog of RAD51 required for homologous recombination (49). Elimination of DNA methylation did not impact growth on any of the tested genotoxic agents.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide redistribution of H3K27me3 is linked to genotoxic stress and defective growth

Basenko

Sasaki

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

H3K9 methylation directs heterochromatin formation by recruiting multiple heterochromatin protein 1 (HP1)-containing complexes that deacetylate histones and methylate cytosine bases in DNA. In Neurospora crassa, a single H3K9 methyltransferase complex, called the DIM-5,-7,-9, CUL4, DDB1 Complex (DCDC), is required for normal growth and development. DCDC-deficient mutants are hypersensitive to the genotoxic agent methyl methanesulfonate (MMS), but the molecular basis of genotoxic stress is unclear. We found that both the MMS sensitivity and growth phenotypes of DCDC-deficient strains are suppressed by mutation of embryonic ectoderm development or Su-(var)3-9; E(z); Trithorax (set)-7, encoding components of the H3K27 methyltransferase Polycomb repressive complex-2 (PRC2). Trimethylated histone H3K27 (H3K27me3) undergoes genome-wide redistribution to constitutive heterochromatin in DCDC- or HP1-deficient mutants, and introduction of an H3K27 missense mutation is sufficient to rescue phenotypes of DCDC-deficient strains. Accumulation of H3K27me3 in heterochromatin does not compensate for silencing; rather, strains deficient for both DCDC and PRC2 exhibit synthetic sensitivity to the topoisomerase I inhibitor Camptothecin and accumulate γH2A at heterochromatin. Together, these data suggest that PRC2 modulates the response to genotoxic stress.

show abstract

Section: Resultsmentioning

confidence: 99%

Genome-wide redistribution of H3K27me3 is linked to genotoxic stress and defective growth

Basenko

Sasaki

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…To determine if ␥H2A is enriched at specific genomic locations in the filamentous fungus Neurospora crassa, we performed ChIP of ␥H2A followed by high-throughput sequencing (ChIP-seq). We used a previously characterized anti-␥H2A antibody that was specific for both yeast and Neurospora ␥H2A proteins (H2A proteins phosphorylated on serine-129 and serine-131, respectively) (66,67). We also performed ChIP-seq experiments to detect two well-characterized chromatin modifications, H3K4me2 and H3K9me3, which are molecular markers of euchromatin and heterochromatin, respectively.…”

Section: Resultsmentioning

confidence: 99%

Heterochromatin Controls γH2A Localization in Neurospora crassa

et al. 2014

View full text Add to dashboard Cite

bIn response to genotoxic stress, ATR and ATM kinases phosphorylate H2A in fungi and H2AX in animals on a C-terminal serine. The resulting modified histone, called ␥H2A, recruits chromatin-binding proteins that stabilize stalled replication forks or promote DNA double-strand-break repair. To identify genomic loci that might be prone to replication fork stalling or DNA breakage in Neurospora crassa, we performed chromatin immunoprecipitation (ChIP) of ␥H2A followed by next-generation sequencing (ChIP-seq). ␥H2A-containing nucleosomes are enriched in Neurospora heterochromatin domains. These domains are comprised of A·T-rich repetitive DNA sequences associated with histone H3 methylated at lysine-9 (H3K9me), the H3K9me-binding protein heterochromatin protein 1 (HP1), and DNA cytosine methylation. H3K9 methylation, catalyzed by DIM-5, is required for normal ␥H2A localization. In contrast, ␥H2A is not required for H3K9 methylation or DNA methylation. Normal ␥H2A localization also depends on HP1 and a histone deacetylase, HDA-1, but is independent of the DNA methyltransferase DIM-2. ␥H2A is globally induced in dim-5 mutants under normal growth conditions, suggesting that the DNA damage response is activated in these mutants in the absence of exogenous DNA damage. Together, these data suggest that heterochromatin formation is essential for normal DNA replication or repair.

show abstract

“…Two signaling pathways have been conserved across evolution: the ATM (ataxia-telangiectasia mutated) and ATR (ATM and Rad3-related) pathways, which involve phosphatidylinositol 3-kinase-like proteins. ATM is activated by detection of double-strand breaks, while ATR recognizes single-stranded DNA regions originating from hairpins, which are caused by halted replication or by damage, for example, that results in pyrimidine dimers (121). A link between the stress kinase and the checkpoint pathways has been suggested.…”

Section: Dna Repairmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

166

195

View full text Add to dashboard Cite

SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

show abstract

ATM and ATR homologes of Neurospora crassa are essential for normal cell growth and maintenance of chromosome integrity

Cited by 12 publications

References 65 publications

Genome-wide redistribution of H3K27me3 is linked to genotoxic stress and defective growth

Genome-wide redistribution of H3K27me3 is linked to genotoxic stress and defective growth

Heterochromatin Controls γH2A Localization in Neurospora crassa

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Contact Info

Product

Resources

About