ATM Promotes RAD51-Mediated Meiotic DSB Repair by Inter-Sister-Chromatid Recombination in Arabidopsis

Yao, Yuan; Li, Xiaojing; Chen, Wanli; Liu, Hui; Mi, Limin; Ren, Di; Mo, Aowei; Lu, Pingli

doi:10.3389/fpls.2020.00839

Cited by 26 publications

(40 citation statements)

References 85 publications

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“…A similar function has also been proposed for ATM in Arabidopsis (Yao et al, 2020). Notably, Arabidopsis atr mutants do not exhibit any meiotic phenotypes but exacerbate the meiotic defects of atm mutants, thus ATR likely plays a supporting role (Culligan and Britt, 2008;Yao et al, 2020).…”

Section: Suppression Of Sister Repairsupporting

confidence: 66%

Phospho-Regulation of Meiotic Prophase

Kar

Hochwagen

2021

Front. Cell Dev. Biol.

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Germ cells undergoing meiosis rely on an intricate network of surveillance mechanisms that govern the production of euploid gametes for successful sexual reproduction. These surveillance mechanisms are particularly crucial during meiotic prophase, when cells execute a highly orchestrated program of chromosome morphogenesis and recombination, which must be integrated with the meiotic cell division machinery to ensure the safe execution of meiosis. Dynamic protein phosphorylation, controlled by kinases and phosphatases, has emerged as one of the main signaling routes for providing readout and regulation of chromosomal and cellular behavior throughout meiotic prophase. In this review, we discuss common principles and provide detailed examples of how these phosphorylation events are employed to ensure faithful passage of chromosomes from one generation to the next.

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Section: Suppression Of Sister Repairsupporting

confidence: 66%

Phospho-Regulation of Meiotic Prophase

Kar

Hochwagen

2021

Front. Cell Dev. Biol.

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“…Orcein staining of meiotic products was performed by referring to (Lei et al, 2020). For the quantification of fluorescent foci of γH2A.X and DMC1, we referred to (Xue et al, 2018;Yao et al, 2020). Images of DAPI-stained chromosome signals and RFP-channeled protein foci signals were merged, and only the foci merged onto chromosomes were counted.…”

Section: Cytology and Quantification Of γH2ax And Dmc1 Focimentioning

confidence: 99%

Heat Stress Interferes with Formation of Double-Strand Breaks and Homolog Synapsis inArabidopsis thaliana

Ning

Liu

Wang

et al. 2020

Preprint

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Meiotic recombination (MR) drives reciprocal exchange of genetic information which allows novel combination of alleles and contributes to genomic diversity of eukaryotes. In this study, we report that heat stress (36-38°C) over fertile threshold fully abolishes crossover occurrence by lowering the formation of SPO11-dependent double-strand breaks (DSBs) in Arabidopsis. The high temperature partially suppresses chromosome fragmentation in the rad51 and syn1 mutants by reducing DSB formation. In addition, we found that although the SYN1-mediated formation of chromosome axis is not influenced by the high temperature, ASY1-associated axial element of synaptonemal complex (SC) is partially compromised, and the ZYP1-dependent central element of axis is severely disrupted, indicating that homology synapsis is another prominent target of high temperature. Besides, we observed that the loading of recombinase DMC1 on chromatin is significantly reduced by high temperatures, suggesting that heat stress interferes with DMC1-dependent MR. Moreover, we found that the numbers of DSB and DMC1 keep stable within a limited range of temperature increase, while decrease when the DSB-processing system reaches threshold to heat. Furthermore, transcriptome study revealed a universal variation of the expression of meiotic genes under low (4°C) and high (32°C) temperatures, suggesting that temperature may influence MR by modulating the transcription of MR-related factors. We also found that the landscape of 5-methylcytosine (5mC) was altered under high temperatures, suggesting that environmental temperature may influence the distribution of MR via reshaped DNA methylation status of chromatin. Taken together, our findings provide evidence shedding light on how environment temperatures influence MR in Arabidopsis.

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“…Hence, it is possible that many gene conversions that occur within these intervals would not be detectable. Alternatively, the lack of increased gene conversion frequency upon targeting MTOPVIB-dCas9 may imply that meiotic DSB repair occurs using the sister chromatid as a template (Cifuentes et al 2013; Yao et al 2020).…”

Section: Discussionmentioning

confidence: 99%

CRISPR targeting of MEIOTIC-TOPOISOMERASE VIB-dCas9 to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis

Gonzalez-Jorge

Hirsz

et al. 2021

Preprint

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During meiosis, homologous chromosomes pair and recombine, which can result in reciprocal crossovers that increase genetic diversity. Crossovers are unevenly distributed along eukaryote chromosomes and show repression in heterochromatin and the centromeres. Within the chromosome arms crossovers are often concentrated in hotspots, which are typically in the kilobase range. The uneven distribution of crossovers along chromosomes, together with their low number per meiosis, creates a limitation during crop breeding, where recombination can be beneficial. Therefore, targeting crossovers to specific genome locations has the potential to accelerate crop improvement. In plants, meiotic crossovers are initiated by DNA double strand breaks (DSBs) that are catalysed by SPO11 complexes, which consist of two catalytic (SPO11-1 and SPO11-2) and two non-catalytic subunits (MTOPVIB). We used the model plant Arabidopsis thaliana to target a dCas9-MTOPVIB fusion protein to the 3a crossover hotspot via CRISPR. We observed that this was insufficient to significantly change meiotic crossover frequency or pattern within 3a. We discuss the implications of our findings for targeting meiotic recombination within plant genomes.

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ATM Promotes RAD51-Mediated Meiotic DSB Repair by Inter-Sister-Chromatid Recombination in Arabidopsis

Cited by 26 publications

References 85 publications

Phospho-Regulation of Meiotic Prophase

Phospho-Regulation of Meiotic Prophase

Heat Stress Interferes with Formation of Double-Strand Breaks and Homolog Synapsis inArabidopsis thaliana

CRISPR targeting of MEIOTIC-TOPOISOMERASE VIB-dCas9 to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis

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