Atmospheric and room temperature plasma mutagenesis and astaxanthin production from sugarcane bagasse hydrolysate by Phaffia rhodozyma mutant Y1

Zhuang, Yuan; Jiang, Gui-Li; Zhu, Ming‐Jun

doi:10.1016/j.procbio.2020.01.003

Cited by 35 publications

(18 citation statements)

References 54 publications

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“…The presence of a thick, rigid and indigestible cell envelope, as well as the lipophilic nature of astaxanthin, are the biggest challenges in the development of efficient methodologies for extraction of this compound (Gogate and Nadar, 2015). Over the last years, several cell wall disruption processes such as chemical (acid extraction), enzymatic and physical or mechanical (ultrasound, microwave, high-pressure homogenization) methods have been evaluated to facilitate the recovery of intracellular carotenoids from X. dendrorhous (Choi et al, 2007;Ni et al, 2008;Michelon et al, 2012;Gogate and Nadar, 2015;Hasan et al, 2016;Urnau et al, 2018;Zhuang et al, 2020). However, those techniques present some disadvantages such as high implementation costs, long extraction time, excessive cell destruction and high cost of purification of the target molecule (Martínez et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The high-rate cell growth with short cultivation cycles, the ability to metabolize waste-based culture media, no seasonal conditions constraints and the easy manipulation of culture conditions to improve yields make the yeast X. dendrorhous an alternative for large scale production of natural astaxanthin ( Wu et al, 2018 ; Villegas-Méndez et al, 2019 ). Generally astaxanthin production from naturally isolated strains of X. dendrorhous is very low (between 200 and 400 μg/g), however, some authors have developed genetic manipulations to get hyper-producer strains with yields up to 10-fold higher than the wild-type strains ( Visser et al, 2003 ; Zhuang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Aguilar-Machado

Delso

Martínez

et al. 2020

Front. Bioeng. Biotechnol.

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The aim of this study was to evaluate the potential of pulsed electric fields (PEF) to improve the extraction of the lipid-soluble astaxanthin from fresh biomass of a wild-type (CECT 11028) and mutant (ATCC 74219) Xanthophyllomyces dendrorhous strain using ethanol as solvent. Inactivation and propidium uptake studies revealed that inactivation is a good index for estimated the proportion of irreversible permeabilized cells when inactivation is higher than 70% in the two strains. Ethanol was ineffective for extracting carotenoids from the PEF-treated cells (20 kV/cm, 135 µs) of the two strains. However, after aqueous incubation of PEF-treated X. dendrorhous ATCC 74219 cells for 12 h, up to 2.4 ± 0.05 mg/g dried weight (d.w.) of carotenoids were extracted in ethanol. From total carotenoid extracted, around 84% corresponded to all-trans astaxanthin. The detection and quantification of esterase activity in the supernatant and the relationship between the percentage of esterase activity quantified and the amount of carotenoids extracted indicate that the extraction of astaxanthin was mediated by enzymatic esterase activity triggered by PEF during incubation. On the other hand, the formation of a large lipid globule into the cytoplasm of PEF-treated X. dendrorhous CECT 11028 cells during aqueous incubation prevented carotenoid extraction. The process developed in this investigation represents a more sustainable and greener method that those previously used for extracting astaxanthin from yeast.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Aguilar-Machado

Delso

Martínez

et al. 2020

Front. Bioeng. Biotechnol.

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“…The YSD YN2 strain was mutated through the ARTP technology according to a previously described method (Zhuang et al 2020;Fan et al 2017). The mature bacterial suspension grown on LB medium was washed with 0.85% sterile saline solution and then diluted to an OD 600 of 0.6-0.8 before mutagenesis.…”

Section: Procedures For Artp Mutagenesismentioning

confidence: 99%

Prospecting the plant growth–promoting activities of endophytic bacteria Franconibacter sp. YSD YN2 isolated from Cyperus esculentus L. var. sativus leaves

Wang

Zhou

et al. 2022

Ann Microbiol

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Purpose Plant growth–promoting (PGP) bacteria are an environment-friendly alternative to chemical fertilizers for promoting plant growth and development. We isolated and characterized a PGP endophyte, YSD YN2, from the leaves of Cyperus esculentus L. var. sativus. Methods Specific PGP characteristics of this strain, such as phosphate solubilization ability, potassium-dissolving ability, siderophore and indole-3-acetic acid (IAA) production, and salt tolerance, were determined in vitro. In addition, positive mutants were screened using the atmospheric and room temperature plasma (ARTP) technology, with IAA level and organic phosphate solubility as indices. Furthermore, the effect of the positive mutant on seed germination, biomass production, and antioxidant abilities of greengrocery seedling was evaluated, and the genome was mined to explore the underlying mechanisms. Results The strain YSD YN2 showed a good performance of PGP characteristics, such as the production of indole acetic acid and siderophores, solubilization ability of phosphate, and potassium-dissolving ability. It was recognized through 16S rRNA sequencing together with morphological and physiological tests and confirmed as Cronobacter sp. The strain exposed to a mutation time of 125 s by ARTP had the highest IAA and organic phosphate (lecithin) concentrations of 9.25 mg/L and 16.50 mg/L, 50.41% and 30.54% higher than those of the initial strain. Inoculation of mutant strain YSD YN2 significantly increased the seed germination, plant growth attributes, and the activities of peroxidase (POD) and superoxide dismutase (SOD), respectively, but decreased the content of malondialdehyde (MDA) significantly compared with the control. Furthermore, genome annotation and functional analysis were performed through whole-genome sequencing, and PGP-related genes were identified. Conclusion Our results indicated that the mutant strain YSD YN2 with PGP characteristics is a potential candidate for the development of biofertilizers.

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“…Recently, several groups developed various host cells, advanced strategies, and tools for the microbial production of terpenoids ( Carsanba et al, 2021 ; Zhang et al, 2021 ; Rinaldi et al, 2022 ). However, as shown in Table 3 , only a few studies explored the biorefining of various forms of organic waste to produce terpenoid flavor and fragrance compounds ( Table 3 ; Yao et al, 2020 ; Zhuang et al, 2020 ; Styles et al, 2021 ; Szotkowski et al, 2021 ; Wei et al, 2021 ). Moreover, the yields achieved in most of these studies was rather low, usually below 1 g/L.…”

Section: Discussionmentioning

confidence: 99%

Efficient production of the β-ionone aroma compound from organic waste hydrolysates using an engineered Yarrowia lipolytica strain

Chen

Wang

et al. 2022

Front. Microbiol.

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This study demonstrates the feasibility of establishing a natural compound supply chain in a biorefinery. The process starts with the biological or chemical hydrolysis of food and agricultural waste into simple and fermentative sugars, followed by their fermentation into more complex molecules. The yeast strain, Yarrowia lipolytica, was modified by introducing high membrane affinity variants of the carotenoid cleavage dioxygenase enzyme, PhCCD1, to increase the production of the aroma compound, β-ionone. The initial hydrolysis process converted food waste or sugarcane bagasse into nutrient-rich hydrolysates containing 78.4 g/L glucose and 8.3 g/L fructose, or 34.7 g/L glucose and 20.1 g/L xylose, respectively. During the next step, engineered Y. lipolytica strains were used to produce β-ionone from these feedstocks. The yeast strain YLBI3120, carrying a modified PhCCD1 gene was able to produce 4 g/L of β-ionone with a productivity of 13.9 mg/L/h from food waste hydrolysate. This is the highest yield reported for the fermentation of this compound to date. The integrated process described in this study could be scaled up to achieve economical large-scale conversion of inedible food and agricultural waste into valuable aroma compounds for a wide range of potential applications.

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Atmospheric and room temperature plasma mutagenesis and astaxanthin production from sugarcane bagasse hydrolysate by Phaffia rhodozyma mutant Y1

Cited by 35 publications

References 54 publications

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Prospecting the plant growth–promoting activities of endophytic bacteria Franconibacter sp. YSD YN2 isolated from Cyperus esculentus L. var. sativus leaves

Efficient production of the β-ionone aroma compound from organic waste hydrolysates using an engineered Yarrowia lipolytica strain

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