Atmospheric moisture influences on conidia development in Podosphaera xanthii through host-plant morphological responses

Itagaki, Kaori; Shibuya, Toshio; Tojo, Motoaki; Endo, Ryosuke; Kitaya, Yoshiaki

doi:10.1007/s10658-013-0309-1

Cited by 13 publications

(6 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Germination at 0 h was evaluated by examining the conidia in the inoculum immediately before inoculation under a digital microscope (VHX-1000; Keyence Corp., Osaka, Japan) with reflected light. The adaxial cotyledon surfaces of three seedlings in each treatment group were observed under a scanning electron microscope (SU-1510; Hitachi High-Technologies Corp., Tokyo, Japan) 24 and 48 h after inoculation, according to Itagaki et al (2014). About 700 to 900 conidia in each treatment group were observed at each time.…”

Section: Methodsmentioning

confidence: 99%

“…Evaluation of haustorial formation 7 d after inoculation. For the evaluation of the late developmental stage of P. xanthii, haustorial formation 7 d after inoculation was observed according to Itagaki et al (2014). Cross-sections (120-mm thick) of leaf pieces (5 mm 2 ) with a representative colony were prepared from five seedlings in each treatment group on a plant microtome (MTH-1; Nippon Medical and Chemical Instruments).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Early Development of Powdery Mildew on Cucumber Leaves Acclimatized to Illumination with Different Red-to-far-red Ratios

Itagaki¹,

Shibuya²,

Tojo³

et al. 2016

horts

Self Cite

View full text Add to dashboard Cite

The development of powdery mildew fungus (Podosphaera xanthii) is suppressed on cucumber (Cucumis sativus L.) seedlings acclimatized to higher red-to-far-red ratio (R:FR) than natural R:FR (≈1.2), but its early development and any limiting factors are still unclear. The present study evaluated conidial germination, initial invasion, and subsequent development of P. xanthii on cucumber seedlings raised under light-emitting diode (LED) lights with R:FRs of 1.2, 5.0, or 10. There were no differences in conidial germination or initial invasion between the treatments, so there was no effect of acclimatization to R:FR on either. But, the development of hyphae, hyphal cells, and haustoria after inoculation were suppressed on seedlings acclimatized to higher R:FR. Because differences occurred only after the initial invasion, nonstructural properties of the host leaves may have affected conidial development. Higher R:FR also suppressed conidial development under natural light filtered through a photo-selective film, which absorbs near-infrared (NIR)-light. However, this effect was reduced when the plants were moved to natural R:FR after inoculation, possibly because of reacclimatization of the seedlings.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Early Development of Powdery Mildew on Cucumber Leaves Acclimatized to Illumination with Different Red-to-far-red Ratios

Itagaki¹,

Shibuya²,

Tojo³

et al. 2016

horts

Self Cite

View full text Add to dashboard Cite

show abstract

“…When the cotyledons had expanded and the first true leaf had begun to emerge, 10 seedlings in each treatment were sprayed with a suspension of P. xanthii spores onto the adaxial surface of the cotyledons (Itagaki et al, 2014;Shibuya et al, 2011). The concentrations were 3.03 • 10 6 spores/mL in trial 1, 0.89 • 10 6 spores/mL in trial 2, and 3.05 • 10 6 spores/mL in trial 3.…”

Section: Methodsmentioning

confidence: 99%

Development of Powdery Mildew Fungus on Cucumber Leaves Acclimatized to Different CO2 Concentrations

Itagaki

Shibuya

Tojo

et al. 2015

horts

Self Cite

View full text Add to dashboard Cite

The present study evaluated the development of powdery mildew fungus (Podosphaera xanthii) on leaves of cucumber (Cucumis sativus L.) acclimatized to different CO2 concentrations ([CO2]) to examine plant–pathogen interactions under the wide range of [CO2] that can occur in greenhouse cultivation. Seedlings of resistant and nonresistant cultivars were acclimatized to reduced (200 µmol·mol−1), ambient (400 µmol·mol−1), or elevated (1000 µmol·mol−1) [CO2]. Powdery mildew spores were inoculated onto the adaxial surface of cotyledons or first true leaves, and colonization was measured after 7 days. Colony density decreased as acclimatization [CO2] increased at the cotyledon stage but increased at the first-true-leaf stage in both cultivars. This result implies that when the effects of [CO2] on plant–pathogen interactions are described, growing stage must be specified. The acclimatization [CO2] was correlated positively with leaf mass per area, dry matter content, and carbon (C) content and negatively with nitrogen (N) content at both stages. Therefore, these leaf properties could not explain the changes in host-plant susceptibility between stages. The effect of acclimatization [CO2] was greater on the resistant cultivar than on the nonresistant cultivar, indicating that the resistant cultivar was more responsive.

show abstract

“…The authors previously reported that conidial development of cucurbit powdery mildew 41 (Podosphaera xanthii) was suppressed on cucumber (Cucumis sativus L.) leaves acclimatized 42 to low humidity (Itagaki et al 2014). This previous research demonstrated that spore 43 germination was not affected, but colony density and haustoria formation 7 days after 44 inoculation were less on leaves whose epidermis had become thicker under lower humidity.…”

Section: Introduction 33mentioning

confidence: 99%

Differences in early hyphal development of Podosphaera xanthii on Cucumis sativus leaves acclimatized to high or low relative humidity

Itagaki

Shibuya

2018

Botany

Self Cite

View full text Add to dashboard Cite

This research investigated early hyphal development of cucurbit powdery mildew (Podosphaera xanthii) on leaves of cucumber (Cucumis sativus L.) acclimatized to different relative humidities to clarify the factors that limit it. Cucumber seedlings were acclimatized to a low (10%) or high (90%) relative humidity (RH) at 30 °C from germination. When the cotyledons had expanded and the first true leaf had begun to emerge, the plants were inoculated, and initial conidial invasion, hyphal development, and secondary haustorial formation were evaluated. The initial invasion of P. xanthii into the epidermis was slower on the low-RH leaves than on the high-RH leaves, suggesting that structural properties, such as greater leaf mass per area, physically limited the development of P. xanthii. The hyphal development and secondary haustoria of conidia that had successfully infected the epidermis were also slower on the low-RH leaves. These post-invasion behaviors indicate that conidial development was also suppressed by nonstructural properties of low-RH leaves such as nutrients, which correlate with nonstructural defense. The reduced leaf N content of the low-RH leaves may in part have inhibited the post-invasion behaviors of P. xanthii.

show abstract

Atmospheric moisture influences on conidia development in Podosphaera xanthii through host-plant morphological responses

Cited by 13 publications

References 39 publications

Early Development of Powdery Mildew on Cucumber Leaves Acclimatized to Illumination with Different Red-to-far-red Ratios

Early Development of Powdery Mildew on Cucumber Leaves Acclimatized to Illumination with Different Red-to-far-red Ratios

Development of Powdery Mildew Fungus on Cucumber Leaves Acclimatized to Different CO2 Concentrations

Differences in early hyphal development of Podosphaera xanthii on Cucumis sativus leaves acclimatized to high or low relative humidity

Contact Info

Product

Resources

About